PhD, Immunophysiology, Kansas State University, 1995
MS, Veterinary Immunology, Chinese Academy of Agricultural Sciences, 1988
DVM, Beijing Agricultural University, 1985
Phone (785) 532-4506
Novel vaccine adjuvants and strategies that lead to the development of better vaccines targeting swine diseases including porcine reproductive and respiratory syndrome (PRRS), classical swine fever virus (CSF), porcine circovirus (PCV), porcine epidemic diarrhea (PED), and other emerging viral diseases of swine.
Selected Publications (1994-2016)
Madera, R., W. Gong, L. Wang, Y. Burakova, K. Lleellish, A. Galliher-Beckley, J. Nietfeld, J.. Henningson, K. Jia, P. Li, J. Bai, J. Schlup, S. McVey, C. Tu, & J. Shi. 2016. Pigs immunized with a novel E2 subunit vaccine are protected from heterologous classical swine fever virus challenge. BMC Veterinary Research 12:197.
Shi, X., X. Liu, Q. Sun, Q. Wang, G. Ma, G. Anderson, J. Bai*, and J. Shi*. 2016. A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses. Journal of Virological Methods 236:258-265.
Huang, C., Y. Du, Z. Yu, Q. Zhang, Y. Liu, J. Tang, J. Shi, and W. Feng. 2016. Highly pathogenic porcine reproductive and respiratory syndrome virus nonstructural protein 4 cleaves VISA to impair antiviral responses mediated by RIG-I-like receptors. Scientific Reports 6:28497.
Gong, W., L. Zhang, Z. Lu, J. Jia, M. Wang, Z. Peng, H. Guo, J. Shi*, and C. Tu*. 2016. Complete genome sequence of a novel classical swine fever virus sub-subgenotype 2.1g isolate from China. Archives of Virology 161:2613-2617.
Gong, W., Z. Lu, L. Zhang, X. Xie, D. Jiang, J. Jia, H. Guo, J. Shi*, and C. Tu*. 2016. In vitro adaptation and genome analysis of a sub-subgenotype 2.1c isolate of classical swine fever virus. Virus Genes 52:651-659.
Gong, W., J. Wu, Z. Lu, L. Zhang, S. Qin, F. Chen, Z. Peng, Q. Wang, L. Ma, A. Bai, H. Guo, J. Shi*, and C. Tu*. 2016. Genetic diversity of subgenotype 2.1 isolates of classical swine fever virus. Infection, Genetics and Evolution 4:218-226.
Galliher-Beckley, A., L.K. Pappan, A. Waters, X. Li, J. Nietfeld, Q. Zhong, S. McVey, S.S. Dritz, and J. Shi. 2015. Characterization of a novel oil-in-water emulsion adjuvant for swine influenza virus and Mycoplasma hyopneumoniae vaccines. Vaccine 33:2903-2908.
Galliher-Beckley, A., X. Li, R. Madera, A. Waters, J. Nietfeld, J. Henningson, D. He, W. Feng, R. Chen, and J. Shi. 2015. Pigs immunized with Chinese highly pathogenic PRRS virus modified live vaccine are protected from challenge with North American PRRSV strain NADC-20. Vaccine 33:3518-3525.
Li, X, A. Galliher-Beckley, L. Pappan, B. Trible, M. Kerrigan, A. Beck, D. Hesse, F. Blecha, J.C. Nietfeld, R.R. Rowland, and J. Shi. 2014. Comparison of host immune responses to homologous and heterologous porcine reproductive and respiratory syndrome virus (PRRSV) challenge in vaccinated and unvaccinated pigs. Biomed Res Int. 2014: 416727.
Chen, X., R. Quan, X. Guo, L. Gao, J. Shi, and W. Feng. 2014. Up-regulation of pro-inflammatory factors by HP-PRRSV infection in microglia: implications for HP-PRRSV neuropathogenesis. Veterinary Microbiology 170(1-2):48-57.
Khoo L., D. Wise, R.D. Berghaus, J. Shi, and A.C. Camus. 2014. Iron status of channel catfish Ictalurus punctatus affected by channel catfish anemia and response to parenteral iron. Diseases of Aquatic Organisms 107(3):241-8.
Li, X., A. Galliher-Beckley, J.C. Nietfeld, H. Huang, X. Sun, and J. Shi. 2013. Peptide nanofiber hydrogel adjuvanted live virus vaccine enhances cross-protective immunity to porcine reproductive and respiratory syndrome virus. Vaccine 31:4508-4515.
Galliher-Beckley, A., L. Lan, S. Aono, L. Wang, and J. Shi. 2013. Caspase-1 activation and mature interleukin-1β release are uncoupled events in monocytes. World Journal of Biological Chemistry 4(2): 30-34.
Li, X., A. Galliher-Beckley, J.C. Nietfeld, K.S. Faaberg, and J. Shi. 2013. MontanideTM Gel01 ST Adjuvant Enhances PRRS Modified Live Vaccine Efficacy by Regulating Porcine Humoral and Cellular Immune Responses. World Journal of Vaccines 3:1-9.
Li, Y., Wang, L., L. Pappan, A. Beckly, and J. Shi. 2012. IL-1β promotes stemness and invasiveness of colon cancer cells through Zeb1 activation. Molecular Cancer 11:87* (*Highly Accessed).
Wang, L., Z. Liu, Y., Li, L. Pappan, A. Beckly, and J. Shi. 2012. IL-1β promotes the development of intestinal epithelial stem cells. Inflammation Research, 61:1085-1092.
Zhou, Z., X. Li, Q. Liu, D. Hu, X. Yue, J. Ni, X. Yu, X. Zhai, A. Galliher-Beckley, N. Chen, J. Shi, and K. Tian. 2012. Complete genome sequence of two novel Chinese virulent porcine reproductive and respiratory syndrome virus variants. Journal of Virology, 86:6373-6374.
Yu, X., N. Chen, L. Wang, J. Wu, Z. Zhou, J. Ni, X. Li, X. Zhai, J. Shi, K. Tian. 2012. New genomic characteristics of highly pathogenic porcine reproductive and respiratory syndrome viruses do not lead to significant changes in pathogenicity. Veterinary Microbiology, 158:291-299.
*Wang, L., Z. Liu, S. Balivada, T. Shrestha, Stefan Bossmann, M. Pyle, L. Pappan, J. Shi*, and D. Troyer. 2012. IL-1β and TGF-β cooperate to reprogram glioma cells toward a glioma stem cell-like phenotype. Stem Cell Research and Therapy. 3:5. (*Co-corresponding author)
Huang, H., J. Shi, J. Laskin, Z. Liu, D.S. McVey, and X. Sun. 2011. Design of a shear-thining recoverable peptide hydrogel from native sequences and application for influenza H1N1 vaccine adjuvant. Soft Matter. 7:8905-8912.
McVey, D.S, and J. Shi. 2010. Vaccination strategies for emerging disease epidemics of livestock. Veterinary Clinics of North America - Food Animal Practice. 26:173-83.
Hu, X., C. Ward, S. Aono, L. Lan, C. Dykstra, R. Kemppainen, E. Morrison, and J. Shi. 2008. Comparative analysis of Xenopus tropicalis hepcidin I and hepcidin II genes. Gene 426:91-97.
Shi, J. 2007. Defensins and Paneth cells in inflammatory bowel disease. Inflammatory Bowel Disease 13:1284-92.
Shi, J., S. Aono, W. Lu, X. Hu, A.J. Ouellette, Y. Ji, L. Wang, S. Lenz, F. van Ginkel, M. Liles, C. Dykstra, E.E. Morrison, and C.O. Elson. 2007. A novel role for defensins in intestinal homeostasis: regulation of IL-1β secretion. The Journal of Immunology 179:1245-1253.
Aono, S., C. Li, G. Zhang, R.J. Kemppainen, J. Gard, W. Lu, X. Hu, D.D. Schwartz, E.E. Morrison, C. Dykstra, and J. Shi. 2006. Molecular and functional characterization of bovine β-defensin-1. Veterinary Immunology and Immunopathology 113:181-190.
Shi, J. and A.C. Camus. 2006. Hepcidins in amphibians and fishes: antimicrobial peptides or iron-regulatory hormones? Developmental and Comparative Immunology 30:746-755.
Shi, J., E. Talbot, D. A. DiMattia, and R. G. Dullea. 2004. The differential effects of IL-1 and TNF-α on proinflammatory cytokines and matrix metalloproteinases expression in human hondrosarcoma cells. Inflammation Research 53:277-89.
Shi, J., G. Zhang, H. Wu, C.R. Ross, F. Blecha, and T. Ganz. 1999. Porcine epithelial β-defensin-1 is expressed in the dorsal tongue at antimicrobial concentration. Infection and Immunity 67:3121-3127.
Shi, J. and T. Ganz. 1998. The role of protegrins and other elastase-activated cathelicidins in the bactericidal properties of porcine inflammatory fluids. Infection and Immunity 66:3611-3617.
Shi, J., C.R. Ross, T. L. Leto, and F. Blecha. 1996. PR-39, a proline-rich antibacterial peptide that inhibits phagocyte NADPH oxidase activity by binding to Src homology 3 domains of p47phox. Proceedings of the National Academy of Sciences USA 93:6014-6018.
Shi, J., C.R. Ross, M.M. Chengappa, and F. Blecha. 1994. Identification of a proline-arginine-rich antibacterial peptide from neutrophils that is analogous to PR-39, an antibacterial peptide from the small intestine. Journal of Leukocyte Biology 56:807-811