PhD, Andrology, University of Delhi, Delhi, India
MPhil, Neuroendocrinology, University of Delhi
Coles Hall 231
The research efforts of my laboratory are directed toward understanding the molecular mechanisms underlying alterations in gene expression in the brain following chronic alcohol exposure. Alcohol use disorders affect an estimated 17 million Americans. Alcohol related problems have a huge economic burden costing the US about $224 billion each year. Alcohol is known to affect different receptor systems in the brain. Among these receptor systems N-methyl-D aspartate (NMDA) receptors play an important role in the development of alcohol dependence and withdrawal syndrome. In my laboratory we are particularly interested in understanding the molecular mechanisms that underlie the changes in gene expression of NMDA receptors following alcohol exposure. Our main focus is on the role of RNA-protein interactions in regulation of NMDA receptor expression. We also study the effect of alcohol exposure on RNA splicing of NMDA receptors. My laboratory uses multi-disciplinary approaches from molecular and cell biology and biochemistry. We perform neuronal cell culture and utilize a mouse model of alcohol dependence.
Anji, A. and Kumari, M. (2011). A cis-acting region in the N-methyl-D-aspartate R1 3'-untranslated region interacts with the novel RNA-binding proteins beta subunit of alpha glucosidase II and annexin A2 – effect of chronic ethanol exposure in vivo. Eur. J. Neurosci. 34:1200-1211
Anji, A. and Kumari, M. (2009). Differentiated P19 cells express NMDA R1 mRNA binding trans-acting proteins and four NMDA R1 splice variants comparable to cultured fetal cortical neurons. J. Neurosci. Res. 87:1591-1601
Anji, A. and Kumari, M. (2008). Supplementing the liquid alcohol diet with chow enhances alcohol intake in C57 BL/6 mice. Drug Alcohol Depend. 97(1-2):86-93
Anji, A. and Kumari, M. (2006). A novel RNA binding protein that interacts with NMDA R1 mRNA: regulation by ethanol. Eur. J. Neurosci. 23(9):2339-2350
Kumari, M., Anji, A., Woods, H., and Ticku, M.K. (2003) The molecular effects of alcohol: Clues to alcohol's enigmatic action! Annals of the New York Academy of Sciences . 993:82-94; discussion 123-4, 2003.
Anji A., Kamran A. Shaik and Meena Kumari (2003) Effect of Ethanol on Lipid-mediated Transfection of Primary Cortical Neurons. Annals of the New York Academy of Sciences . 993:95-102; discussion 123-4, 2003.
Kumari, M. Differential effects of chronic ethanol treatment on NMDA R1 splice variants in cultured fetal cortical neurons. J. Biol. Chem. 276:29764-29771, 2001.
Kumari, M. and Anji, A. Sequencing through GC-rich and AT-rich sequences with the SequiTherm Excel II DNA sequencing kit. Epicentre Forum, 8(3):9, 2001.
Anji, A., Sullivan-Hanley, N.R., Kumari, M. and Hensler, J.G. The role of protein kinase C in the regulation of serotonin-2A receptor expression. J. Neurochem. 77:589-597, 2001.
Anji, A., Kumari, M., Sullivan-Hanley, N.R., Bryan, G.L. and Hensler, J.G. Regulation of 5HT2A receptor mRNA levels and binding sites in rat frontal cortex by agonist and antagonist treatment. Neuropharmacology. 39:1996-2000, 2000.
Ethanol and expression of NMDA R1 splice variants in fetal cortical neurons. Neurosci. Abstr., 2001.
Evidence of the presence of four NR1 splice variants in mouse cortical neurons. Alcoholism: Clinical and Exp. Res. 25(Suppl):21, 2001.