Diagnostic Medicine/Pathobiology

Flow Cytometry Lab

The Flow Cytometry laboratory is housed in Mosier Hall, Room K208 of the College of Veterinary Medicine complex. This multi-user core laboratory is equipped with state of the art flow cytometry analyzers by Becton Dickinson (LSR Fortessa X-20 and FACSCalibur), and a multiplex MagPix instrument.

Hours of Operation: M-F 8 am–9 pm, Subject to building access, Book instruments via online scheduler.

Usage Fees

Instrument and Fee Rates (internal/external)

FACSMelody

Internal Fees: Available soon
External Fees: Available soon

LSR Fortessa X20

Internal Fees: $25/15 min
External Fees: $37.50/15 min

Assisted flow on Analyzers

$30/15 min

MagPix Setup (by staff)

Internal Fees: $30/plate
External Fees: $45/plate

TRAINING PACKAGES (2 hrs)

$100

Subsidized by Department of Diagnostic Medicine/Pathobiology/KSVDL.

New users will be strongly encouraged to sign up for a minimum of 2 hours of training. Training will cost $100, and will accommodate up to 2 trainees per session. The training sessions will cover instrument set-up, data acquisition, trouble-shooting, and introduction to analysis software.

 

E-mail or call for consultation

 

Flow Cytometry Core Manager

Joel Sanneman

jsanneman@vet.k-state.edu
Phone:
(785)-341-2145

 

Instruments

 

LSR Fortessa X-20 Special Order Research Product Flow Cytometer

Lasers: 405nm, 488nm, 561nm, 640nm
Fluorescent Markers: FITC, GFP, Alexa 488, Alexa 546, Alexa 647, PerCP-Cy5.5, APC, Alexa 700, APC-Cy7, PE, PI, 7-AAD, PerCP, PETxRed, PE-Cy5, PE-Cy7, BV421, PacBlue, BV510, AmCyan, BV650, BV785, or any equivalent wavelength fluorophores.

LSR Fortessa X-20 Special Order Research Product Flow Cytometer

The Fortessa X-20 is located in Mosier Hall K208A and is equipped with 4 non-linear lasers (405, 488, 561, 640 nm) and filter configurations allowing for detection of up to 12 colors plus Fwd and Ssc parameters. The cytometer utilizes FACSDiva v8.0 acquisition software and generates FCS files compatible with all commercial analysis programs. Routine CS&T calibration allows for standardization between experiments, increasing the reliability of data generated over time. This cytometer accepts samples in 5.0 mL falcon tube format with a recommended sample volume of approx. 300+ uL.

FACSMelody

The Melody resides in Mosier Hall K208 and is configured for high speed, viable, aseptic sorts from a wide range of cell types. It has three lasers (405 violet, 488 blue, and 561 yellow-green) and nine photomultiplier detectors. Detection channels include forward scatter (FSC), side scatter (SSC), plus filter configurations for the detection of fluorescence to include 448/45, 428/45, 582/15, 613/18YG, 697/58, 783/56YG, 488/15, 527/32, 700/54, 510/10, 545/20, 528/45, and 755/LP. Software control is via BD FACSChorus. Samples can be maintained through a temperature control ranch of 4 to 37 degrees Celsius, complete with agitation. Collection reservoirs include 1.5 mL centrifuge tubes, 5 mL tubes, slides, and multi-well plates (6, 24, 48, 96, and 384).

MagPix

The MagPix by Millipore can analyze up to 50 analytes in a single sample. Examples are cytokine, chemokine, and serological panels. Call for consultation and quote for usage.

Additional educational resource: Tutorials on flow cytometry