Diagnostic Medicine/Pathobiology

Flow Cytometry Lab

The Flow Cytometry laboratory is housed in Mosier Hall, Room K208 of the College of Veterinary Medicine complex. This multi-user core laboratory is equipped with state of the art flow cytometry analyzers by Becton Dickinson (LSR Fortessa X-20 and FACSCalibur), and a multiplex MagPix instrument.

Hours of Operation: M-F 8–5 p.m., Call to schedule a time on the instruments

Usage Fees

Instrument and Fee Rates (internal/external)

BD FACSCalibur

Internal Fees: $20/15 min
External Fees: $30/15 min

LSR Fortessa X20

Internal Fees: $25/15 min
External Fees: $37.50/15 min

Assisted flow on Analyzers

$30/15 min

MagPix Setup (by staff)

Internal Fees: $30/plate
External Fees: $45/plate

TRAINING PACKAGES (2 hrs)

$100

Subsidized by Department of Diagnostic Medicine/Pathobiology/KSVDL.

New users will be strongly encouraged to sign up for a minimum of 2 hours of training. Training will cost $100, and will accommodate up to 2 trainees per session. The training sessions will cover instrument set-up, data acquisition, trouble-shooting, and introduction to analysis software.

 

E-mail or call for consultation

Director

Nora Springer, Assistant Professor

nlspring@vet.k-state.edu
Phone: 785-532-4818

Flow Cytometry Core Manager

Joel Sanneman

jsanneman@vet.k-state.edu
Phone:
(785)-341-2145

 

Instruments

FACS CALIBUR

Lasers: 488nm, 642nm
Fluorescent Markers: FITC, GFP, Alexa 488, PE, PI, PerCP, 7-AAD, Pe-Cy7, APC, Alexa 647

LSR Fortessa X-20 Special Order Research Product Flow Cytometer

Lasers: 405nm, 488nm, 561nm, 640nm
Fluorescent Markers: FITC, GFP, Alexa 488, Alexa 546, Alexa 647, PerCP-Cy5.5, APC, Alexa 700, APC-Cy7, PE, PI, 7-AAD, PerCP, PETxRed, PE-Cy5, PE-Cy7, BV421, PacBlue, BV510, AmCyan, BV650, BV785, or any equivalent wavelength fluorophores.

FACSCalibur

The FACSCalibur is equipped with two lasers that emit light at 488 nm and 630 nm. There are three detectors (FL1, FL2, FL3) for the 488 laser and one detector (FL4) for the 630 laser. This two laser cytometer allows the operator to analyze up to 4-color and 2 size parameters of cells or fluorescent-labeled particles. FACSCalibur is interfaced with a Macintosh computer and a publication quality color printer. Cell Quest and ModFit analysis software is available on two computers to analyze flow cytometry data including DNA and proliferation assays.

LSR Fortessa X-20 Special Order Research Product Flow Cytometer

The Fortessa X-20 is equipped with 4 non-linear lasers (405, 488, 561, 640 nm) and filter configurations allowing for detection of up to 12 colors plus Fwd and Ssc parameters. The cytometer utilizes FACSDiva v8.0 acquisition software and generates FCS files compatible with all commercial analysis programs. Routine CS&T calibration allows for standardization between experiments, increasing the reliability of data generated over time. This cytometer accepts samples in 5.0 mL falcon tube format with a recommended sample volume of approx. 300+ uL.

MagPix

The MagPix by Millipore can analyze up to 50 analytes in a single sample. Examples are cytokine, chemokine, and serological panels. Call for consultation and quote for usage.

Additional educational resource: Tutorials on flow cytometry