Diagnostic Medicine/Pathobiology
Flow Cytometry Lab
The Flow Cytometry laboratory is housed in Mosier Hall, Room K208 of the College of Veterinary Medicine complex. This multi-user core laboratory is equipped with state of the art flow cytometry analyzers by Becton Dickinson (LSR Fortessa X-20 and FACSCalibur).
Hours of Operation: M-F 8 am–9 pm, Subject to building access, Book instruments via online scheduler.
Usage Fees
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FACSMelodyInternal Fees: Available soon |
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LSR Fortessa X20Internal Fees: $25/15 min |
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Assisted flow on Analyzers$30/15 min |
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TRAINING PACKAGES (2 hrs)$100 |
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Subsidized by Department of Diagnostic Medicine/Pathobiology/KSVDL.
New users will be strongly encouraged to sign up for a minimum of 2 hours of training. Training will cost $100, and will accommodate up to 2 trainees per session. The training sessions will cover instrument set-up, data acquisition, trouble-shooting, and introduction to analysis software.
E-mail or call for consultation
Flow Cytometry Core Manager
Joel Sanneman
jsanneman@vet.k-state.edu
Phone: (785)-341-2145
Instruments |
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LSR Fortessa X-20 Special Order Research Product Flow CytometerLasers: 405nm, 488nm, 561nm, 640nm |
LSR Fortessa X-20 Special Order Research Product Flow Cytometer
The Fortessa X-20 is located in Mosier Hall K208A and is equipped with 4 non-linear lasers (405, 488, 561, 640 nm) and filter configurations allowing for detection of up to 12 colors plus Fwd and Ssc parameters. The cytometer utilizes FACSDiva v8.0 acquisition software and generates FCS files compatible with all commercial analysis programs. Routine CS&T calibration allows for standardization between experiments, increasing the reliability of data generated over time. This cytometer accepts samples in 5.0 mL falcon tube format with a recommended sample volume of approx. 300+ uL.
FACSMelody
The Melody resides in Mosier Hall K208 and is configured for high speed, viable, aseptic sorts from a wide range of cell types. It has three lasers (405 violet, 488 blue, and 561 yellow-green) and nine photomultiplier detectors. Detection channels include forward scatter (FSC), side scatter (SSC), plus filter configurations for the detection of fluorescence to include 448/45, 428/45, 582/15, 613/18YG, 697/58, 783/56YG, 488/15, 527/32, 700/54, 510/10, 545/20, 528/45, and 755/LP. Software control is via BD FACSChorus. Samples can be maintained through a temperature control ranch of 4 to 37 degrees Celsius, complete with agitation. Collection reservoirs include 1.5 mL centrifuge tubes, 5 mL tubes, slides, and multi-well plates (6, 24, 48, 96, and 384).