Diagnostic Medicine/Pathobiology

Flow Cytometry Lab

The Flow Cytometry laboratory is housed at the Biomedical Core Facility in Mosier Hall CR210 of the College of Veterinary Medicine complex. This multi-user core laboratory is equipped with state of the art flow cytometry analyzers by Becton Dickinson (FACSCalibur).

Hours of Operation: M-F 8 am–9 pm, Subject to building access, Book instruments via online scheduler.

Usage Fees

Instrument and Fee Rates (internal/external)

FACSMelody

Internal Fees: $100
External Fees: $153

Assisted flow on Analyzers

$30/15 min

TRAINING PACKAGES (2 hrs)

$100

Subsidized by Department of Diagnostic Medicine/Pathobiology/KSVDL.

New users will be strongly encouraged to sign up for a minimum of 2 hours of training. Training will cost $100, and will accommodate up to 2 trainees per session. The training sessions will cover instrument set-up, data acquisition, trouble-shooting, and introduction to analysis software.

E-mail or call for consultation

Joel Sanneman - Flow Cytometry Core Manager
jsanneman@vet.k-state.edu
Phone: (785)-341-2145

Tej Shrestha
tbs3@vet.k-state.edu
785-532-4248

Instruments
Cytek Aurora Cytometer The Cytek Aurora is a spectral-based four-laser (UV/V/B/R) (54 + 3 Channel) cytometer features that can accommodate large panels and is equipped with an autoloader for 40-tuberacks and plates. This instrument has provisions for autofluorescence background subtraction.
BD FACSMelody Cell Sorter The Melody resides in Mosier Hall K208 and is configured for high speed, viable, aseptic sorts from a wide range of cell types. It has three lasers (405 violet, 488 blue, and 561(yellow-green) and nine photomultiplier detectors. Detection channels includeforward scatter (FSC), side scatter (SSC), plus filter configurations for the detection of fluorescence to include 448/45, 428/45, 582/15, 613/18YG, 697/58, 783/56YG, 488/15, 527/32, 700/54, 510/10, 545/20, 528/45, and 755/LP. Software control is via BD FACSChorus. Samples can be maintained through a temperature control ranch of 4 to 37 degrees Celsius, complete with agitation. Collection reservoirs include 1.5 mL centrifuge tubes, 5 mL tubes, slides, and multi-well plates (6, 24, 48, 96, and 384).

Instruments

Cytek Aurora Cytometer
The Cytek Aurora is a spectral-based four-laser (UV/V/B/R) (54 + 3 Channel) cytometer features that can accommodate large panels and is equipped with an autoloader for 40-tuberacks and plates. This instrument has provisions for autofluorescence background subtraction.

BD FACSMelody Cell Sorter
The Melody resides in Mosier Hall K208 and is configured for high speed, viable, aseptic sorts from a wide range of cell types. It has three lasers (405 violet, 488 blue, and 561(yellow-green) and nine photomultiplier detectors. Detection channels includeforward scatter (FSC), side scatter (SSC),
plus filter configurations for the detection of fluorescence to include 448/45, 428/45, 582/15, 613/18YG, 697/58, 783/56YG, 488/15, 527/32, 700/54, 510/10, 545/20, 528/45, and 755/LP. Software control is via BD FACSChorus. Samples can be maintained through a temperature control ranch of 4 to 37 degrees Celsius, complete with agitation. Collection reservoirs include 1.5 mL centrifuge tubes, 5 mL tubes, slides, and multi-well plates (6, 24, 48, 96, and 384).