Professor, Arbovirology

PhD, Liverpool School of Tropical Medicine, Liverpool UK
MS, Colorado State University
BS, Colorado State University

Office: 1041 Pat Roberts Hall
Phone: 785-532-1333
Email: dlvanlan@vet.k-state.edu

Research

My research is focused on zoonotic arboviruses most of which require high containment facilities. Some current projects are:

1) Development of Countermeasures for a Japanese Encephalitis Introduction in the United States. Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic flavivirus that can cause fatal infections in animals and humans. The purpose of this project is to develop novel vaccines and therapeutics which could be used in case of an introduction of this virus into the U.S.

2) Biology of Mosquito Vectors of Arbovirus Transmission Associated with Emerging Disease Threats of Livestock. The purpose of this project is to facilitate research to enhance the understanding of arbovirus maintenance, transmission, and infection/disease in mosquitoes, swine, and sheep. The overall goals of this research program is to understand how arboviruses adapt to insect and animal hosts and how this knowledge can be used to break the cycle of transmission and to develop better countermeasures to prevent the transmission of zoonotic diseases to human hosts.

3) Control of Emerging Bunyaviruses. Arboviruses in the family of Bunyaviridae utilize a wide range of arthropod vectors, e.g. mosquitoes, midges, or ticks to transmit pathogens that have a high impact on human and veterinary public health. The overall goal of this project is to establish and evaluate a vaccine platform for the rapid generation of new vaccines to protect livestock against emerging bunyaviruses.

Currently funded projects:

1) NIH Center on Emerging and Zoonotic Infectious Diseases (CEZID) – “'Identifying Novel Antiviral Targets to Inhibit Flaviviral Replication Using CRISPR-Cas9” - PI: D. Vanlandingham

2) Corporate Sponsor – “Investigation of inactivated SARS-CoV-2 for diagnostic tools” – PI: D. Vanlandingham

3) Corporate Sponsor – “Evaluation of SARS-CoV-2 inactivation in blood products” - PI: D. Vanlandingham

4) USDA APHIS – “Diagnostic Methods of Emerging Disease Threats of Livestock”- NBAF Laboratorian Training Program (NLTP) - PI: D. Vanlandingham

5) USDA APHIS – “High Containment Laboratory Practices and Technical Course” - PI: D. Vanlandingham

6) Corporate Sponsor – “Evaluation of West Nile virus inactivation in blood products” – PI: D. Vanlandingham

7) USDA - Research Education and Economics - Agricultural Research Service (ARS) - 58-3020-9-030 – “Animal Studies in BSL-3 Containment at Pat Roberts Hall for Comparative Zoonotic Arbovirus Studies” - Co-PI: D. Vanlandingham

8) DHS Science and Technology (S&T) - Center of Excellence for Emerging Zoonotic Animal Disease (CEEZAD) “Biological Safety Level 3 (BSL-3) Training Program” - Co-I: D. Vanlandingham

Teaching

I am very active in teaching graduate students and veterinary students. I team-teach VDMP851 Veterinary Virology to second year DVM students. I have developed courses focused on High Containment Transboundary Animal Disease (TAD) research (DMP690, 691, 846, 893, 895, and 995). We also have three active training programs: 1) USDA APHIS sponsored program titled “NBAF Laboratorian Training Program”, aimed at teaching future diagnosticians to support veterinary diagnostic laboratories; 2) International training program titled “High Containment Laboratory Practices and Techniques Course” sponsored by USDA APHIS aimed at supporting leaders of non-U.S. high containment laboratories; and 3) Presenting the first week of the annual CEEZAD BSL-3 Training TAD Summer Program.

NBAF Laboratorian Training Program

Our agreement with the USDA APHIS is designed to build technical skills and expertise to fill the workforce needs of the Foreign Animal Disease Diagnostic Laboratory (FADDL) at the new National Bio and Agro-defense Facility (NBAF) in Manhattan, KS and the National Veterinary Services Laboratories (NVSL) in Ames, IA. This training program will help address the critical need for a skilled technical workforce that will be required when NBAF opens.

Courses Taught

DMP690 - Essential Practices for BSL-3 (1 credit) (2014, 2016, 2017, 2019, 2020, 2021)

DMP691 - Introduction to High Containment Research Topics and Techniques (3 credits) (2014, 2016, 2017, 2019, 2020, 2021)

DMP846 - Foundations in Biosecurity (2 credits) (2016, 2017, 2019, 2020)

DMP893 - Principles of Biosafety and Biocontainment (3 credits) (2017, 2018, 2019)

DMP822 - Veterinary Virology (3 credits, 6 lectures) (2015 - 2020)

VDMP851 - Veterinary Virology (3 credits, 6 lectures) (2021)

DMP813 - Select Agent Studies (3 credits) (2017, 2018, 2019, 2021)

DMP895-A - Transboundary Animal Diseases (3 credits) (2017, 2018)

Service

Director - BRI high containment insectary (ACL-3) and the ACL-1 laboratory

Service on Committees:

Kansas State University Academic Affairs Committee Chair (2019, 2020)

Kansas State University Faculty Senate University Library Committee (2019-present)

Kansas State University Graduate Council Member (2017-present)

Kansas State University Academic Affairs Committee Member (2017-present)

Kansas State University IBC Committee Member (2013-present)

College of Veterinary Medicine Faculty Council Member (2020-present)

College of Veterinary Medicine Promotion and Tenue Committee (2020-present)

Fellowships

Fellow, Royal Entomological Society of London

Fellow, Marty Vanier and Bob Krause Biosecurity Research Institute Research Fellow

Link to NCBI MyBibliography for a complete list of published work.

Selected publications (12 most recent from a total of 91)

Huang Y-J.S., Higgs S. and Vanlandingham D.L. 2019. Arbovirus-Mosquito Vector-Host Interactions and the Impact on Transmission and Disease Pathogenesis of Arboviruses. Front. Microbiol. 10:22. DOI: 10.3389/fmicb.2019.00022. PMID: 30728812. PMCID: PMC6351451.

Huang Y-J.S., Higgs S. and Vanlandingham D.L. 2019. Emergence and Re-emergence of Mosquito-borne Arboviruses. Curr. Opin. Virol. 34; 104-109. DOI: doi.org/10.1016/j.coviro.2019.01.001. PMID: 30743191.

Valiant, W., Mattapallil, M., Higgs, S., Huang, Y-J., Vanlandingham, D., Lewis, M., and Mattapallil, J. 2019. Simultaneous Coinfection of Macaques with Zika and Dengue Viruses Does not Enhance Acute Plasma Viremia but Leads to Activation of Monocyte Subsets and Biphasic Release of Pro-inflammatory Cytokines. Sci Rep. 9:1; article 7877. DOI: 10.1038/s41598-019-44323-y. PMID: 31133721. PMCID: PMC6536518.

Ko, S-Y., Akahata, W., Yang, E. S., Kong, W-P., Burke, C. W., Honnold, S. P., Nichols, D. K., Huang, Y-J. S., Schieber, G. L., Carlton, K., DaSilva, L., Traina-Dorge, V., Vanlandingham, D. L., Tsybovsky, Y., Stephens, T., Baxa, U., Higgs, S., Roy, C. J., Glass, P. J., Mascola, J. R., Nabel, G. J., Rao, S. S. 2019. A virus-like particle vaccine prevents equine encephalitis virus infection in nonhuman primates. Science Translational Medicine. 11:492. DOI: 10.1126/scitranslmed.aav3113. PMID: 31092692.

Ayers, V. B., Huang, Y-J.S., Lyons, A.C., Park, S.L., Dunlop, J.I., Unlu, I., Kohl, A., Higgs, S., Blitvich, B.J., and Vanlandingham, D.L. 2019. Infection and transmission of Cache Valley virus by Aedes albopictus and Aedes aegypti mosquitoes. Parasit Vectors. DOI: 10.1186/s13071-019-3643-0. PMID: 31366369. PMCID: PMC6670168.

Young, C.L., Lyons, A.C., Hsu, W-W., Vanlandingham, D.L., Park, S.L., Doerfler, A.N., Ayers, V.B., Hettenbach, S.M., Zelenka, A.M., Cool, K.R., Peterson, G.J., Higgs, S., Huang, Y-J.S. 2019. Protection of swine by potent neutralizing anti-Japanese encephalitis virus monoclonal antibodies derived from vaccination. Antiviral Research. 174. DOI: 10.1016/j.antiviral.2019.104675. PMID: 31825852.

Burke, C.W., Wiley, M.R., Beitzel, B.F., Gardner, C.L., Huang, Y-J., Piper, A.E., Vanlandingham, D.L., Higgs, S., Palacios, G., Glass, P.J. 2020. Complete Coding Sequence of Western Equine Encephalitis Virus Strain Fleming, Isolated from a Human Case. Microbiology Resource Announcements. 9 (1). e01223-19; DOI: 10.1128/MRA.01223-19. PMID: 31896634. PMCID: PMC6940286.

Winkler, E.S., Shrihari, S., Hykes, B.L. Jr, Handley, S.A., Andhey, P.S., Huang, Y.S., Swain, A., Droit, L., Chebrolu, K.K., Mack, M., Vanlandingham, D.L., Thackray, L.B., Cella, M., Colonna, M., Artyomov, M.N., Stappenbeck, T.S., and Diamond, M.S. 2020. The Intestinal Microbiome Restricts Alphavirus Infection and Dissemination through a Bile Acid-Type I IFN Signaling Axis. Cell. 2020;182(4):901-918.e18. DOI:10.1016/j.cell.2020.06.029. PMID: 32668198. PMCID: PMC7483520.

Huang, Y.S., Vanlandingham, D.L., Bilyeu, A.N., Sharp, H.M, Hettenbach, S.M., Higgs, S. 2020. SARS-CoV-2 failure to infect or replicate in mosquitoes: an extreme challenge. Sci Rep. 10(1):11915. Published 2020 Jul 17. DOI:10.1038/s41598-020-68882-7. PMID: 32681089. PMCID: PMC7368071.

Higgs, S., Vanlandingham, D.L., Huang, Y.S., Thangamani, S. 2021. The use of Arthropod-borne Challenge Models in BSL-3Ag and BSL-4 Biocontainment. ILAR Journal. 2021; ilab013. DOI: https://doi.org/10.1093/ilar/ilab013.

Huang, Y.S., Bilyeu, A.N., Hsu, W-W., Hettenbach, S.M., Willix, J.L., Stewart, S.C., Higgs, S., Vanlandingham, D.L. Treatment with Dry Hydrogen Peroxide Accelerates the Decay of Severe Acute Syndrome Coronavirus-2 on Non-porous Hard Surfaces. American Journal of Infection Control. ePub July 14, 2021. DOI: https://doi.org/10.1016/j.ajic.2021.07.006.

Park, S.L., Huang, Y.S., Lyons, A.C., Ayers, V.B., Hettenbach, S.M., McVey, D.S., Noronha, L.E., Burton, K.R., Hsu, W., Higgs, S., Vanlandingham, D.L. Mosquito saliva modulates Japanese encephalitis virus infection in domestic pigs. Front. Virol. 1:724016. DOI: https://doi.org/10.3389/fviro.2021.724016.