College of Veterinary Medicine

Ben Hause

Ben HauseResearch Assistant Professor

PhD (2013), South Dakota State University
MS (2001), University of Minnesota
BA (1998), University of Minnesota

Office: L-227 Mosier Hall
Phone: (785) 532-4278
Email: bhause@vet.k-state.edu

Service

My service interests include the utilization and development of advanced molecular biology techniques to identify and characterize established, emerging and novel animal viruses. Using Next Generation Sequencing (NGS), we are able to fully characterize the genomes of viruses isolated on cell culture and often times directly from clinical samples. We also use NGS as a virus detection method, employing metagenomic sequencing techniques to concurrently detect and genetically characterize viruses present in a clinical sample. These techniques do not require a priori sequence information, allowing identification of novel pathogens and characterization of the viral population.

Research

Much of my research involves follow up studies based on results generated from NGS. As new or uncommon viruses are detected, we seek to determine their relevance to animal health. These studies often include epidemiological and pathological investigations. One such virus was originally isolated from pigs with influenza-like illness and subsequently shown to be common in bovine respiratory disease. Detailed genetic and antigenic studies suggested that this virus likely represents a new genus in the Orthomyxoviridae family, proposed influenza D virus. On-going research on this virus is focusing on its role in the bovine respiratory disease complex.

My other research interest is developing novel vaccines for animal health. Using reverse genetics, we are able to genetically engineer viruses such as influenza virus, allowing us to attenuate pathogenicity while maintaining immunogenicity. Our long term goal is to generate efficacious platform vaccine technologies which can be used for multiple pathogens.

Patents and Applications

8,137,953 - Lactic acid producing yeast cells having nonfunctional L- or D-lactate ferric cytochrome C oxidoreductase cells
7,534,597 - Methods and materials for the production of L-lactic acid in yeast
20140023681 - Attenuated swine influenza vaccines and methods and making and use thereof
20130209507 - Rotavirus subunit vaccines and methods of making and use thereof
20130195915 - Influenza C virus and vaccine
20100137551 - Methods and materials for the production of D-lactic acid in yeast

Publications

  1. Anbalagan S, Spaans T, Hause BM. 2014. Genome sequence of the novel reassortant mammalian orthoreovirus strain MRV00304/13 isolated from a bovine with diarrhea from the U.S. Genome Announcement 2(3):e00451-14..
  1. Anbalagan S, Hause BM. 2014.Characterization of epizootic hemorrhagic disease virus from a bovine with clinical disease with high spatial-temporal similarity to white-tailed deer isolates. Archives of Virology, Epub ahead of print 2014 May 23.
  1. Anbalagan S, Hause BM. 2014. First identification of porcine enterovirus G in the United States. PLoS One 9(5):e97517.
  1. HauseBM, CollinEA, LiuR,, Huang B, ShengZ, LuW,Wang D,SimonsonRR, LiF. 2014. Characterization of a Novel Influenza Virus in Cattle and Swine: Implication for a New Genus with a Bovine Reservoir. mBio 5(2):e00031-14.
  1. Anbalagan S, Cooper E, Klumper P, Simonson R, Hause BM. 2014. Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment. Journal of General Virology 95:434-441.
  1. Cooper E, Anbalagan S, Klumper P, Scherba G, Simonson R, Hause BM. 2014. Mobuck virus genome sequence and phylogenetic analysis: Identification of a novel Orbivirus isolated from a white-tailed deer in Missouri, USA. Journal of General Virology 95:110-116.
  1. Zheng S, Ran Z, Wang D, Hoppe A, Simonson RR, Chakravarty S, Hause BM, Li F. 2014. Genomic and evolutionary characterization of a novel influenza C-like virus from swine. Archives of Virology 159:249-255.
  1. Miller MW, Hause BM, Killion HJ, Edwards WH, Wolfe L. 2013. Phylogenetic and epidemiological relationships among Pasteurellaceae from Colorado Bighorn sheep herds. Journal of Wildlife Diseases 49(3):653-660.
  1. Hause BM, Ducatez M, Collin EA, Ran Z, Liu R, Sheng Z, Armien A, Kaplan B, Chakravarty S, Hoppe AD, Webby RJ, Simonson RR, Li F. 2013. Isolation of a novel swine influenza virus from Oklahoma in 2011 which is distantly related to human influenza C viruses. PLoS Pathogens 9(2):e1003176.
  1. Hause BM, Stine DL, Sheng Z, Wang Z, Chakravarty S, Simonson RR, Li F. 2012. Migration of the swine influenza virus δ-cluster hemagglutinin N-linked glycosylation site from N142 to N144 results in loss of antibody cross-reactivity. Clinical Vaccine Immunology 19(9):1457-1464.
  1. Sun Z, Huber VC, McCormick K, Kaushik RS, Boon AC, Zhu L, Hause BM, Webby RJ, Fang Y. 2012. Characterization of a porcine intestinal epithelial cell line for influenza virus production. Journal of General Virology 93(Pt.9):2008-2016.
  1. Hause BM, Collin EA, Ran Z, Zhu L, Webby RJ, Simonson RR, Li F. 2012. In vitro reassortment between endemic H1N2 and 2009 H1N1 pandemic swine influenza viruses generates attenuated viruses. PLoS One 7(6):e39177.
  1. Patterson AR, Johnson JK, Ramamoorthy S, Hesse RA, Murtaugh MP, Puvanendiran S, Pogranichniy RM, Erickson GA, Carman S, Hause BM, Meng XJ, Opriessnig T. 2011. Interlaboratory comparison of porcine circovirus-2 indirect immunofluorescent antibody test and enzyme linked immunosorbent assay results on experimentally infected pigs. Journal of Veterinary Diagnostic Investigation 23(2):206-212.
  1. Ducatez MF, Hause BM, Stigger-Rosser E, Darnel D, Corzo C, Juleen K, Simonson R, Brockwell-Staats C, Rubrum A, Wang D, Webb A, Crumpton JC, Lowe J, Gramer M, Webby RJ. 2011. Reassortment of endemic swine influenza with pandemic A/H1N1 (2009) in swine herds in the United States. Emerging and Infectious Diseases 17(9):1624-1629.
  1. Hause BM, Oleson TA, Stine DL, Bey RF, Simonson RR. 2011. Genetic and antigenic characterization of recent human-like (δ-cluster) swine influenza virus isolates. Journal of Swine Health and Production 19(5):268-276.
  1. Hause BM, Oleson TA, Bey RF, Stine DL, Simonson RR. 2010. Antigenic categorization of contemporary H3N2 swine influenza virus isolates using a high-throughput serum neutralization assay. Journal of Veterinary Diagnostic Investigation 22(3):352-359.