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College of Veterinary Medicine

Mark L. Weiss

Mark L. WeissProfessor

PhD, Biology, University of Pennsylvania, 1986

Phone: 785-532-4520
Email: weiss@vet.k-state.edu

Weiss joined the faculty at Kansas State University in 1991. He is co-discoverer of mesenchymal stromal cells within umbilical cord Wharton's jelly, is the Director of the Midwest Institute for Comparative Stem Cell Biotechnology, Coordinator of the Stem Cell Certificate program (MS certificate program) and is a member of the Terry C. Johnson Center for Basic Cancer Research.

Weiss' research focus is on stem cell biotechnology. His lab successfully produced various stem cell lines such as rat embryonic stem cells and cells derived from umbilical cord or other tissues with the intent of using this technology to advance cellular therapy and regenerative medicine. His lab is focused upon producing promising cellular therapeutics for regenerative medicine. For example, mesenchymal stromal cells have been tested in a variety of rodent preclinical disease models including neurodegenerative diseases such as Parkinson's disease, heart disease such as myocardial infarction, and cancer. Based upon the immune properties of Wharton' s jelly derived mesenchymal stromal cells, the lab is working towards clinical trials with colleagues at Kansas State University, the University of Kansas and University of Minnesota.

In addition, the Weiss lab focuses upon the mechanisms of pluripotency in rat embryonic stem cells. In this area the focus is to efficiently produce new rat models of human disease using gene targeting in rat embryonic stem cells. Till date, the lab has produced chimeric rats using the techniques described by our collaborator, Dr. Qilong Ying (USC), and a variety of tools for quality control and validation.

Courses taught:

AP 747 Veterinary Physiology II
AP 850 Stem Cell Techniques (course coordinator)
AP 711 Stem Cells and Comparative Biomedicine (course coordinator)
AP 995b Stem Cell Journal Club (course coordinator)
AP 773 Bioinstrumentation lab (course coordinator)
VAP 891S Stem Cells and Comparative Biomedicine (course coordinator)

Seminars:

Work-related links:

Adrienne Wright’s SCD 2020 journal cover

Javier Abello’s Theranostics 2019 graphical abstract

Links to work-related 3D models on the NIH 3D Print Exchange:

https://3dprint.nih.gov/discover/3DPX-003850

https://3dprint.nih.gov/discover/3DPX-003849

https://3dprint.nih.gov/discover/3DPX-003524

https://3dprint.nih.gov/discover/3DPX-011231

https://3dprint.nih.gov/discover/3DPX-011230

https://3dprint.nih.gov/discover/3DPX-011229

https://3dprint.nih.gov/discover/3dpx-013011

https://3dprint.nih.gov/discover/3DPX-014177

Selected Publications:

A Phase I Study to Evaluate Two Doses of Wharton’s Jelly-Derived Mesenchymal Stromal Cells for the Treatment of De Novo High-Risk or Steroid-Refractory Acute Graft Versus Host Disease. RP Soder, B Dawn, ML Weiss, N Dunavin, S Weir, J Mitchell, M Li, L Shune, AK Singh, S Ganguly, M Morrison, H Abdelhakim, AK Godwin, S Abhyankar, J McGuirk. Stem Cell Reviews and Reports. 2020.  https://doi.org/10.1007/s12015-020-10015-8

A Protocol for the Isolation, Culture, and Cryopreservation of Umbilical Cord-Derived Canine Mesenchymal Stromal Cells: Role of cell attachment in long-term maintenance. Adrienne Wright, Larry Snyder, Kaori Knights, Hong He, Nora L. Springer, James Lillich, & Mark L. Weiss.  Stem Cells and Development.  Stem Cells Dev. 2020 Jun 1;29(11):695-713. doi: 10.1089/scd.2019.0145. Epub 2020 Apr 22. PMID: 32148170NOTE: cover.

Human Umbilical Cord Mesenchymal Stromal Cell- Derived Exosomes Exhibit Homing Properties in K7M2-Tumor Bearing Mice. Javier Abello, Tuyen Duong Thanh Nguyen, Ramesh Marasini, Santosh Aryal and Mark Louis Weiss Theranostics 2019; 9(8): 2325-2345. doi:10.7150/thno.30030.

Defining hydrogel properties to instruct lineage- and cell-specific mesenchymal differentiation Ben P. Hung, Jenna N. Harvestine, Augustine M. Saiz, Tomas Gonzalez-Fernandez, David E. Sahar, Mark L. Weiss, and J. Kent Leach. Biomaterials. 2019 Jan;189:1-10. doi: 10.1016/j.biomaterials.2018.10.024. PMID: 30384124,

Biomimetic 3D Printing of Hierarchical and Interconnected Porous Hydroxyapatite Structures with High Mechanical Strength for Bone Cell Culture.  Song X, Tetik H, Jirakittsonthon T, Parandous P, Lei S, Lin D.  Advanced Eng Material, 21: 1800678, 2019, https://doi.org/10.1002/adem.201800678

Human mesenchymal stromal cell-derived extracellular vesicles attenuate aortic aneurysm formation and macrophage activation via microRNA-147.  Spinosa M, Lu G, Su G, Bontha SV, Gehrau R, Salmon MD, Smith JR, Weiss ML, Mas VR, Upchurch GR Jr, Sharma AK.  FASEB J. 2018 May 29:fj201701138RR. doi: 10.1096/fj.201701138RR. PMID: 29812968

Mesenchymal stromal cell-derived extracellular vesicles attenuate lung ischemia-reperfusion injury and enhance reconditioning of donor lungs after circulatory death. Stone ML, Zhao Y, Robert Smith J, Weiss ML, Kron IL, Laubach VE, Sharma AK. Respir Res. 2017 Dec 21;18(1):212. doi: 10.1186/s12931-017-0704-9.PMID: 29268735

Human Umbilical Cord Mesenchymal Stromal Cell Isolation, Expansion and Characterization. Smith JR, Cromer A, Weiss ML. Curr Protoc Stem Cell Biol. 2017 May 16;41:1F.18.1-1F.18.23. doi: 10.1002/cpsc.24. PMID: 28510332

Mesenchymal Stem Cells Attenuate NADPH Oxidase-Dependent High Mobility Group Box 1 Production and Inhibit Abdominal Aortic Aneurysms. Sharma AK, Salmon MD, Lu G, Su G, Pope NH, Smith JR, Weiss ML, Upchurch GR Jr. Arterioscler Thromb Vasc Biol. 2016 May;36(5):908-18. doi: 10.1161/ATVBAHA.116.307373. Epub 2016 Mar 17. PMID: 26988591

Administration of Adipose-derived Stromal Vascular Fraction and Platelet Rich Plasma in Dogs with Coxofemoral Osteoarthritis.  David A. Upchurch, Walter C Renberg, James K. Roush, George A. Milliken, Mark L. Weiss,  Am J Vet Res. 2016 Sep;77(9):940-51. doi: 10.2460/ajvr.77.9.940. PMID: 27580105

One Health overview, facilitating advances in comparative medicine and translational research. Stroud C, Dmitriev I, Kashentseva E, Bryan JN, Curiel DT, Rindt H, Reinero C, Henry CJ, Bergman PJ, Mason NJ, Gnanandarajah JS, Engiles JB, Gray F, Laughlin D, Gaurnier-Hausser A, Wallecha A, Huebner M, Paterson Y, O'Connor D, Treml LS, Stannard JP, Cook JL, Jacobs M, Wyckoff GJ, Likins L, Sabbagh U, Skaff A, Guloy AS, Hays HD, LeBlanc AK, Coates JR, Katz ML, Lyons LA, Johnson GC, Johnson GS, O'Brien DP, Duan D, Calvet JP, Gandolfi B, Baron DA, Weiss ML, Webster DA, Karanu FN, Robb EJ, Harman RJ. Clin Transl Med. 2016 Aug;5(Suppl 1):26. doi: 10.1186/s40169-016-0107-4.cts:

Manufacturing Cells for Clinical Use. Weiss ML, Rao MS, Deans R, Czermak P. Stem Cells Int. 2016;2016:1750697. doi: 10.1155/2016/1750697. Epub 2016 Jun 12. PMID: 27382370. NB: Was Lead editor for special edition.

Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use. Petry F, Smith JR, Leber J, Salzig D, Czermak P, Weiss ML. Stem Cells Int. 2016;2016:4834616. doi: 10.1155/2016/4834616. Epub 2016 Feb 8. PMID: 26977155

Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method. Smith JR, Pfeifer K, Petry F, Powell N, Delzeit J, Weiss ML. Stem Cells Int. 2016;2016:6810980. doi: 10.1155/2016/6810980. Epub 2016 Feb 4. PMID: 26966439 

Wharton’s Jelly Derived Mesenchymal Stromal Cells as a Promising Cellular Therapeutic strategy for the Management of Graft-versus-Host Disease), JP McGuirk, J.R. Smith, M.Zuniga, and ML Weiss.  Pharmaceuticals (Basel). 2015 Apr 16;8(2):196-220. Review. PMID: 25894816

Directed migration of embryonic stem cells derived neural cells in an applied electric field. Li Y, Weiss ML, Yao L.  Stem Cells Reviews and Reports 10: 653–662, 2014. eprint: May 2014 DOI 10.1007/s12015-014-9518-z.

Functional Reconstruction of Tracheal Defects by Protein-Loaded, Cell-Seeded, Fibrous Constructs in Rabbits. Ott LM, Vu CH, Farris AL, Fox KD, Galbraith RA, Weiss ML, Weatherly RA, Detamore MS. Tissue Eng Part A. 2015 Sep;21(17-18):2390-403. PMID: 26094554

Defining Pluripotency of Rat Induced Pluripotent Stem Cells.  P. Bui, J. Hong, P. Rajanahalli and M.L. Weiss.  Cell Prolif. 2013 Apr;46(2):119-20. PMID: 23510465.

Wharton's Jelly or Bone Marrow Mesenchymal Stromal Cells Improve Cardiac Function Following Myocardial Infarction for More Than 32 Weeks in a Rat Model: A Preliminary Report. Lopez Y, Lutjemeier B, Seshareddy K, Trevino EM, Hageman KS, Musch TI, Borgarelli M, Weiss ML. Curr Stem Cell Res Ther. 2013 Jan 1;8(1):46-59. PMID: 23270633

A focused microarray for screening rat embryonic stem cell lines. Hong J, He H, Bui P, Ryba-White B, Rumi MA, Soares MJ, Dutta D, Paul S, Kawamata M, Ochiya T, Ying QL, Rajanahalli P, Weiss ML. Stem Cells Dev. 2013 Feb 1;22(3):431-43. doi: 10.1089/scd.2012.0279. Epub 2012 Sep 28. PMID: 22889370

Derivation and Characterization of Embryonic Stem Cells Lines Derived from Transgenic Fischer 344 and Dark Agouti Rats. J. Hong, H. He, and M.L. Weiss. Stem Cells and Development. Published Oct 13, 2011 PMID: 21995453

Evaluating the impact of oxygen concentration and plating density on human Wharton's jelly-derived mesenchymal stromal cells. Y. López, K. Seshareddy, E. Trevino, J. Cox, and M.L. Weiss. The Open Tissue Engineering and Regenerative Medicine Journal, in press.

Promising cellular therapeutics for prevention or management of graft-versus-host disease (a review). McGuirk JP, Weiss ML. Placenta. 2011 Jun 7. PMID:21658764

Meeting report of the first conference of the International Placenta Stem Cell Society (IPLASS). Parolini O, Alviano F, Betz AG, Bianchi DW, Götherström C, Manuelpillai U, Mellor AL, Ofir R, Ponsaerts P, Scherjon SA, Weiss ML, Wolbank S, Wood KJ, Borlongan CV. Placenta. 2011 May 14. PMID:21575989

Xenogeneic transplantation of equine testicular cells into seminiferous tubules of immunocompetent rats. Ferrer MS, Lutjemeier BJ, Koopman T, Pierucci-Alves F, Weiss ML. Theriogenology. 2011 Apr 15;75(7):1258-64. PMID:21316749

Musculoskeletal tissue engineering with human umbilical cord mesenchymal stromal cells. Wang L, Ott L, Seshareddy K, Weiss ML, Detamore MS. Regen Med. 2011 Jan;6(1):95-109. PMID:21175290

A comparison of human bone marrow-derived mesenchymal stem cells and human umbilical cord-derived mesenchymal stromal cells for cartilage tissue engineering. Wang L, Tran I, Seshareddy K, Weiss ML, Detamore MS. Tissue Eng Part A. 2009 Aug;15(8):2259-66. PMID:19260778

Effect of initial seeding density on human umbilical cord mesenchymal stromal cells for fibrocartilage tissue engineering. Wang L, Seshareddy K, Weiss ML, Detamore MS. Tissue Eng Part A. 2009 May;15(5):1009-17. PMID:18759671

Immune properties of human umbilical cord Wharton's jelly-derived cells. Weiss ML, Anderson C, Medicetty S, Seshareddy KB, Weiss RJ, VanderWerff I, Troyer D, McIntosh KR. Stem Cells. 2008 Nov;26(11):2865-74. PMID:18703664

Combination treatment of human umbilical cord matrix stem cell-based interferon-beta gene therapy and 5-fluorouracil significantly reduces growth of metastatic human breast cancer in SCID mouse lungs. Rachakatla RS, Pyle MM, Ayuzawa R, Edwards SM, Marini FC, Weiss ML, Tamura M, Troyer D. Cancer Invest. 2008 Aug;26(7):662-70. PMID:18608205

Method to isolate mesenchymal-like cells from Wharton's Jelly of umbilical cord. Seshareddy K, Troyer D, Weiss ML. Methods Cell Biol. 2008;86:101-19. PMID:18442646

Wharton's jelly-derived cells are a primitive stromal cell population. Troyer DL, Weiss ML. Stem Cells. 2008 Mar;26(3):591-9. PMID:18065397

Stem cells in the umbilical cord. Weiss ML, Troyer DL. Stem Cell Rev. 2006;2(2):155-62. PMID:17237554