Mark L. Weiss
PhD, Biology, University of Pennsylvania, 1986
Weiss joined the faculty at Kansas State University in 1991. He is co-discoverer of mesenchymal stromal cells within umbilical cord Wharton's jelly, is the Director of the Midwest Institute for Comparative Stem Cell Biotechnology, Coordinator of the Stem Cell Certificate program (MS certificate program) and is a member of the Terry C. Johnson Center for Basic Cancer Research.
Weiss' research focus is on stem cell biotechnology. His lab successfully produced various stem cell lines such as rat embryonic stem cells and cells derived from umbilical cord or other tissues with the intent of using this technology to advance cellular therapy and regenerative medicine. His lab is focused upon producing promising cellular therapeutics for regenerative medicine. For example, mesenchymal stromal cells have been tested in a variety of rodent preclinical disease models including neurodegenerative diseases such as Parkinson's disease, heart disease such as myocardial infarction, and cancer. Based upon the immune properties of Wharton' s jelly derived mesenchymal stromal cells, the lab is working towards clinical trials with colleagues at Kansas State University, the University of Kansas and University of Minnesota.
In addition, the Weiss lab focuses upon the mechanisms of pluripotency in rat embryonic stem cells. In this area the focus is to efficiently produce new rat models of human disease using gene targeting in rat embryonic stem cells. Till date, the lab has produced chimeric rats using the techniques described by our collaborator, Dr. Qilong Ying (USC), and a variety of tools for quality control and validation.
AP 747 Veterinary Physiology II
AP 839 Biomedical Methods (instructor)
AP 850 Stem Cell Techniques (course coordinator)
AP 711 Stem Cells and Comparative Biomedicine (course coordinator)
AP 995b Stem Cell Journal Club (course coordinator)
AP 773 Bioinstrumentation lab (course coordinator)
VAP 891S Stem Cells and Comparative Biomedicine (course coordinator)
Links to work-related 3D models on the NIH 3D Print Exchange:
Selected Publications: Click here for Weiss' Google Scholar page
Procoagulant Activity of Umbilical Cord-Derived Mesenchymal Stromal Cells' Extracellular Vesicles (MSC-EVs). A. Wright, O. L. Snyder, H. He, L. K. Christenson, S. Fleming and M. L. Weiss. Int J Mol Sci 2023 Vol. 24 Issue 11 Accession Number: 37298168 PMCID: PMC10252357 DOI: 10.3390/ijms24119216.
Detection of Embryonic Trisomy 21 by 11 Weeks Using Maternal Plasma Cell Free RNA: Discovery, Confirmation, and Validation. CP Weiner, ML Weiss, H Zhou, A Synglaki, KH Nicolaides, Y Dong. Diagnostics, 12(6), 1410, 2022. published eprint: doi: 10.3390/diagnostics12061410. PMID: 35741220 PMCID: PMC9221829
Maternal Plasma RNA in First Trimester Nullipara for the Prediction of Spontaneous Preterm Birth ≦ 32 Weeks: Validation Study. CP Weiner, H Zhou, H Cuckle, A Syngelaki, KH Nicolaides, ML Weiss, Y Dong. Biomedicines 2023 Vol. 11 Issue 4. Accession Number: 37189767 PMCID: PMC10135717 DOI: 10.3390/biomedicines11041149
Evaluation of a Maternal Plasma RNA Panel Predicting Spontaneous Preterm Birth and Its Expansion to the Prediction of Preeclampsia. CP Weiner, H Cuckle, ML Weiss, I Buhimschi, Y Dong, H Zhou, R Ramsey, R Egerman, and C Buhimschi. Diagnostics 12(6), 1327; 2022. https://doi.org/10.3390/diagnostics12061327.
Effect of conditioned media storage temperature on extracellular vesicles isolated from human umbilical cord-derived mesenchymal stromal cells. A Wright, OL Snyder II, H He, ML Weiss. Int. J. Mol. Sci. 2022, 23, 7716. https://doi.org/10.3390/ ijms23147716. PMCID: PMC9320900
A Phase I Study to Evaluate Two Doses of Wharton’s Jelly-Derived Mesenchymal Stromal Cells for the Treatment of De Novo High-Risk or Steroid-Refractory Acute Graft Versus Host Disease. RP Soder, B Dawn, ML Weiss, N Dunavin, S Weir, J Mitchell, M Li, L Shune, AK Singh, S Ganguly, M Morrison, H Abdelhakim, AK Godwin, S Abhyankar, J McGuirk. Stem Cell Reviews and Reports. 2020. https://doi.org/10.1007/s12015-020-10015-8
A Protocol for the Isolation, Culture, and Cryopreservation of Umbilical Cord-Derived Canine Mesenchymal Stromal Cells: Role of cell attachment in long-term maintenance. Adrienne Wright, Larry Snyder, Kaori Knights, Hong He, Nora L. Springer, James Lillich, & Mark L. Weiss. Stem Cells and Development. Stem Cells Dev. 2020 Jun 1;29(11):695-713. doi: 10.1089/scd.2019.0145. Epub 2020 Apr 22. PMID: 32148170NOTE: cover.
Human Umbilical Cord Mesenchymal Stromal Cell- Derived Exosomes Exhibit Homing Properties in K7M2-Tumor Bearing Mice. Javier Abello, Tuyen Duong Thanh Nguyen, Ramesh Marasini, Santosh Aryal and Mark Louis Weiss Theranostics 2019; 9(8): 2325-2345. doi:10.7150/thno.30030.
Defining hydrogel properties to instruct lineage- and cell-specific mesenchymal differentiation Ben P. Hung, Jenna N. Harvestine, Augustine M. Saiz, Tomas Gonzalez-Fernandez, David E. Sahar, Mark L. Weiss, and J. Kent Leach. Biomaterials. 2019 Jan;189:1-10. doi: 10.1016/j.biomaterials.2018.10.024. PMID: 30384124,
Biomimetic 3D Printing of Hierarchical and Interconnected Porous Hydroxyapatite Structures with High Mechanical Strength for Bone Cell Culture. Song X, Tetik H, Jirakittsonthon T, Parandous P, Lei S, Lin D. Advanced Eng Material, 21: 1800678, 2019, https://doi.org/10.1002/adem.201800678
Human mesenchymal stromal cell-derived extracellular vesicles attenuate aortic aneurysm formation and macrophage activation via microRNA-147. Spinosa M, Lu G, Su G, Bontha SV, Gehrau R, Salmon MD, Smith JR, Weiss ML, Mas VR, Upchurch GR Jr, Sharma AK. FASEB J. 2018 May 29:fj201701138RR. doi: 10.1096/fj.201701138RR. PMID: 29812968
Mesenchymal stromal cell-derived extracellular vesicles attenuate lung ischemia-reperfusion injury and enhance reconditioning of donor lungs after circulatory death. Stone ML, Zhao Y, Robert Smith J, Weiss ML, Kron IL, Laubach VE, Sharma AK. Respir Res. 2017 Dec 21;18(1):212. doi: 10.1186/s12931-017-0704-9.PMID: 29268735
Human Umbilical Cord Mesenchymal Stromal Cell Isolation, Expansion and Characterization. Smith JR, Cromer A, Weiss ML. Curr Protoc Stem Cell Biol. 2017 May 16;41:1F.18.1-1F.18.23. doi: 10.1002/cpsc.24. PMID: 28510332
Mesenchymal Stem Cells Attenuate NADPH Oxidase-Dependent High Mobility Group Box 1 Production and Inhibit Abdominal Aortic Aneurysms. Sharma AK, Salmon MD, Lu G, Su G, Pope NH, Smith JR, Weiss ML, Upchurch GR Jr. Arterioscler Thromb Vasc Biol. 2016 May;36(5):908-18. doi: 10.1161/ATVBAHA.116.307373. Epub 2016 Mar 17. PMID: 26988591
Administration of Adipose-derived Stromal Vascular Fraction and Platelet Rich Plasma in Dogs with Coxofemoral Osteoarthritis. David A. Upchurch, Walter C Renberg, James K. Roush, George A. Milliken, Mark L. Weiss, Am J Vet Res. 2016 Sep;77(9):940-51. doi: 10.2460/ajvr.77.9.940. PMID: 27580105
One Health overview, facilitating advances in comparative medicine and translational research. Stroud C, Dmitriev I, Kashentseva E, Bryan JN, Curiel DT, Rindt H, Reinero C, Henry CJ, Bergman PJ, Mason NJ, Gnanandarajah JS, Engiles JB, Gray F, Laughlin D, Gaurnier-Hausser A, Wallecha A, Huebner M, Paterson Y, O'Connor D, Treml LS, Stannard JP, Cook JL, Jacobs M, Wyckoff GJ, Likins L, Sabbagh U, Skaff A, Guloy AS, Hays HD, LeBlanc AK, Coates JR, Katz ML, Lyons LA, Johnson GC, Johnson GS, O'Brien DP, Duan D, Calvet JP, Gandolfi B, Baron DA, Weiss ML, Webster DA, Karanu FN, Robb EJ, Harman RJ. Clin Transl Med. 2016 Aug;5(Suppl 1):26. doi: 10.1186/s40169-016-0107-4.cts:
Manufacturing Cells for Clinical Use. Weiss ML, Rao MS, Deans R, Czermak P. Stem Cells Int. 2016;2016:1750697. doi: 10.1155/2016/1750697. Epub 2016 Jun 12. PMID: 27382370. NB: Was Lead editor for special edition.