Lihua Wang

Lihua WangResearch Assistant Professor of

Virology and Vaccine Immunology

Ph.D, 2006, Chinese Center for Disease Control and Prevention, China
Postdoc, Medical College of Wisconsin and University of Alabama at Birmingham, 2009

230 Coles Hall

Research Interests

Development of novel vaccines, vaccine adjuvants and diagnostic assays for high impact swine infectious diseases including classical swine fever (CSF), African swine fever (ASF), porcine reproductive and respiratory syndrome (PRRS), porcine pseudorabies (PPV), porcine circovirus (PCV), and other emerging viral diseases of swine.

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Selected Publications

Wang L.*, Mi S., Madera R., Li Y., Gong W., Tu C., Shi J. 2022. A Novel Competitive ELISA for Specifically Measuring and Differentiating Immune Responses to Classical Swine Fever C-Strain Vaccine in Pigs. Viruses. 14:1544.

Mi S., Wang L., Li H, Bao F., Madera R., Shi X., Zhang L., Mao Y., Yan R., Xia X., Gong W., Shi J. and Tu C. 2022 Characterization of monoclonal antibodies that specifically differentiate field isolates from vaccine strains of classical swine fever virus. Front. Immunol. 13:930631.

Shi J., Wang L., McVey D.S. 2021. Of pigs and men: the best-laid plans for prevention and control of swine fevers. Animal Frontiers. 11(1):6–13.

Wang L.*, Madera R., Li Y., McVey D.S., Drolet B.S., Shi J. 2020. Recent Advances in the Diagnosis of Classical Swine Fever and Future Perspectives. Pathogens. 9(8):658.

Wang L., Mi S., Madera R., Ganges L., Borca M.V., Ren J., Cunningham C., Cino-Ozuna A.G., Li H., Tu C., Gong W., Shi J. 2020. A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post–vaccination monitoring. BMC Vet Res. 16:14.

Li Y., Fu S., Guo X., Li X., Li M., Wang L., Gao X., Lei W., Cao L., Lu Z., He Y., Wang H., Zhou H., Liang G. 2019. Serological Survey of Getah Virus in Domestic Animals in Yunnan Province, China. Vector Borne Zoonotic Dis. 19 (1):59-61.

Li X., Pei Z., Bai Y., Wang L., Shi J., Tian K. 2018. Phenotypic characterization of porcine IFNγ-producing lymphocytes in porcine reproductive and respiratory syndrome virus vaccinated and challenged pigs. Virol Sin. 33(6):524-530.

Burakova Y., Madera R., Wang L., Buist S., Lleellish K., Schlup J., Shi J. 2018. Food-Grade Saponin Extract as an Emulsifier and Immunostimulant in Emulsion-Based Subunit Vaccine for Pigs. J Immunol Res. 2018:8979838.

Wang L., Madera R., Burakova Y., Buist S., Sang Y., Nietfeld J., Henningson J., Galliher-Beckley A., Gong W., Tu C., Shi J. 2018. Construction of Recombinant Porcine Reproductive and Respiratory Syndrome Virus Expressing CSFV E2 Glycoprotein. Int J Vaccine Res. 3(1):1-4.

Wang L., Shi J. 2018. PRRSV Vaccine: Challenges and Prospective. Int J Vac & Im Sys. 3 (1): 1-5.

Laughlin R.C., Madera R., Peres Y., Berquist B.R., Wang L., Buist S., Burakova Y., Palle S., Chung C.J., Rasmussen M.V., Martel E., Brake D.A., Neilan J.G., Lawhon S.D., Adams L.G., Shi J., Marcel S. 2018. Plant-made E2 glycoprotein single-dose vaccine protects pigs against classical swine fever. Plant Biotechnol J. 17(2):410-420.

Madera R.F., Wang L., Gong W., Burakova Y., Buist S., Nietfeld J., Henningson J., Ozuna A.G.C., Tu C., Shi J. 2018. Towards the development of a one-dose classical swine fever subunit vaccine: antigen titration, onset and duration of immunity. J Vet Sci. 19(3):393-405.

Kim P.K., Keum S.J., Osinubi M.O.V., Franka R., Shin J.Y, Park S.T., Kim M.S., Park M.J., Lee S.Y., Carson W., Greenberg L., Yu P., Tao X., Wang L., Tang Q., Liang G., Shampur M., Rupprecht C.E., Chang S.J. 2017. Development and characterization of novel chimeric monoclonal antibodies for broad spectrum neutralization of rabies virus; PLoS One. 12(10):e0186380.

Liang G., Li X., Gao X., Fu S., Wang H., Li M., Lu Z., Zhu W., Lu X., Wang L., Cao Y., He Y., Lei W. 2017. Arboviruses and their related infections in China: A comprehensive field and laboratory investigation over the last 3 decades. Rev Med Virol. 28(1): 10.1002/rmv.1959.

Li X., Galliher-Beckley A., Wang L., Nietfeld J., Feng W., Shi J. 2017. Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20. Virol J. 11:73-82.

Wang L., Fu S., Cao Y., Zhang H., Feng Y., Yang W., Nie K., Ma X., Liang G.. 2017. Discovery and genetic analysis of novel coronaviruses in least horseshoe bats in southwestern China. Emerg Microbes Infect. 6 (3):e14.

Madera R., Gong W., Wang L., Burakova Y., Lleellish K., Galliher-Beckley A., Nietfeld J., Henningson J., Jia K., Li P., Bai J., Schlup J., McVey S., Tu C., Shi J. 2016. Pigs immunized with a novel E2 subunit vaccine are protected from heterologous classical swine fever virus challenge. BMC Vet Res. 12(1):197.

Yang M., Ke Y., Wang X., Ren H., Liu W., Lu H., Zhang W., Liu S., Chang G., Tian S., Wang L., Huang L., Liu C., Yang R., Chen Z. 2016. Development and Evaluation of a Rapid and Sensitive EBOV-RPA Test for Rapid Diagnosis of Ebola Virus Disease. Sci Rep. 6:26943.

Wang L., Shi J. 2016. Sequencing for Surveillance of Emerging Infectious Diseases: from Laboratory to Field. Infect Dis Transl Med. 2(1):1-2.

Shi L., Fu S., Wang L., Li X., Gu D., Liu C., Zhao C., He J., Liang G.. 2016. Surveillance of mosquitoe-borne infectious diseasesin febrile travelers entering China via Shenzhen ports, China, 2013. Travel Med Infect Dis. 14(2):123-30.

Zhang J., Wang J., Wang L., Fu S., Li M., Zhao G., Zhu W., Wang D., Liang G. 2015. Molecular Characterization and Seroprevalence in Pigs of SC0806, a Cat Que Virus Isolated from Mosquitoes in Sichuan Province, China. Vector Borne Zoonotic Dis. 15(7):423-31.

Wang L., Fu S., Cao L, Lei W., Cao Y., Song J., Tang Q., Zhang H., Feng Y., Yang W., Liang G. 2015. Isolation and Identification of a Natural Reassortant Mammalian Orthoreovirus from Least Horseshoe Bat in China. PLoS One. 10(3): e0118598.

Sang S., Chen B., Wu H., Yang Z., Di B., Wang L., Tao X., Liu X., Liu Q.. 2015. Dengue is still an imported disease in China: A case study in Guangzhou. Infect Genet Evol. 32:178-190.

Wang L., Tang Q., Liang G. 2014. Rabies and rabies virus in wildlife in mainland China, 1990-2013. Int J Infect Dis. 25:122-129.

Zhang H., Zhang Y., Yang W., Tao X., Li H., Ding J., Feng Y., Yang D., Zhang J., He J., Shen X., Wang L., Zhang Y., Song M., Tang Q. 2014. Molecular epidemiology of re-emergent rabies in Yunnan province, southwestern China. Emerg Infect Dis. 20(9):1433-42.

Wang L., Cao Y., Tang Q., Liang G. 2013. Role of the blood-brain barrier in rabies virus infection and protection. Protein Cell. 4(12):901-3.

Wang L., Wu H., Tao X., Li H., Rayner S., Liang G., Tang Q. 2013. Genetic and Evolutionary Characterization of RABVs from China Using the Phosphoprotein Gene. Virol J. 10:14.

Wu H. , Wang L. , Tao X., Li H., Rayner S., Liang G., Tang Q. 2013. Genetic diversity and molecular evolution of the rabies virus matrix protein gene in China. Infect Genet Evol. 16:248-53.

Wang L., Lv X., Zhai Y., Fu S., Wang D., Rayner S., Tang Q., Liang G.. 2012. Genomic Characterization of a Novel Virus of the Family Tymoviridae Isolated from Mosquitoes. PLoS One. 7(7): e39845.

Du J., Tang Q., Huang Y., Rodney W., Wang L., Liang G. 2011. Development of recombinant rabies viruses vectors with Gaussia luciferase reporter based on Chinese vaccine strain CTN181. Virus Res. 160:82-8.

Pan X., Liu H., Wang H., Fu S., Liu H., Zhang H., Li M., Gao X., Wang J., Sun X., Lu X., Zhai Y., Meng W., He Y., Wang H., Han N., Wei B., Wu Y., Feng Y., Yang D., Wang L., Tang Q., Xia G., Kurane I., Rayner S., Liang G. 2011. The Emergence of Genotype I of Japanese Encephalitis Virus as the Dominant Genotype in Asia. J Virol. 85(19):9847-53.

Wang L., Fu S., Zhang H., Ye X., Yu D., Deng Z., Yuan J., Zhai Y., Li M., Lv Z., Chen W., Jiang H., Gao X., Cao Y., Wang H., Tang Q., Liang G. 2010. Identification and isolation of Genotype-I J apanese Encephalitis virus from encephalitis patients. Virol J. 7:345.

Zhu W., Wang L., Yang Y., Jia J., Fu S., Feng Y., He Y., Li J., Liang G. 2010. Interaction of E2 Glycoprotein with Heparan Sulfate Is Crucial for Cellular Infection of Sindbis Virus. PLoS One 5(3): e9656.

He J., Kraft A., Fan J., Van Dyke M., Wang L., Bose M., Khanna M., Metallo J., Henrickson K. 2010. Simultaneous Detection of CDC Category "A" DNA and RNA Bioterrorism Agents by Use of Multiplex PCR & RT-PCR Enzyme Hybridization Assays. Viruses. 1(3): 441-459.

Che P., Wang L., Li Q. 2009. The development, optimization and validation of an assay for high throughput antiviral drug screening against Dengue virus. Int J Clin Exp Med. 2:363-373.

Kumar S., Wang L., Fan J., Kraft A., Bose M., Tiwari S., Van Dyke M., Haigis R., Luo T., Ghosh M., Tang H., Haghnia M., Mather E., Weisburg W., Henrickson K. 2008. Detection of 11 Common Viral and Bacterial Pathogens Causing Community-Acquired Pneumonia or Sepsis in Asymptomatic Patients Using a Multiplex RT-PCR Assay with Manual (Enzyme Hybridization) or Automated Detection (Electronic Microarray). J Clin Microbiol. 46(9):3063-72.

Wang L., Fu S., Wang H., Liang X., Cheng J., Jing H., Cai G., Li X., Ze W., Lv X., Wang H., Zhang D., Feng Y., Yin Z., Sun X., Shui T., Li M., Li Y., Liang G. 2007. Japanese encephalitis outbreak, Yuncheng, China, 2006. Emerg Infect Dis. 13:1123-1125.

Li Y., Wang L., Li S., Chen X., Shen Y., Zhang Z., He H., Xu W., Shu Y., Liang G., Fang R., Hao X. 2007. Seco-pregnane steroids target the subgenomic RNA of alphavirus-like RNA viruses. Proc Natl Acad Sci U S A. 104(19): 8083-8088.