College of Veterinary Medicine

Deryl Troyer

Deryl TroyerProfessor

DVM, Kansas State University, 1972
PhD, Kansas State University, 1985

Research interests

Traditional chemo or radiation cancer therapy results in numerous off-target effects that themselves can sometimes be life threatening. Therefore, more targeted yet effective therapies are needed, along with improved detection of early stages to improve treatment outcomes. The focus of the Troyer laboratory is to develop stem or defensive cells as delivery vehicles for targeted antimicrobial or cancer therapy. Dr. Troyer is a co-discoverer of stem cells isolated from the matrix of umbilical cords (UCMS cells), and these cells traffic to tumors and have a potent intrinsic ability to attenuate tumors. Dr. Troyer's laboratory has successfully used engineered UCMS cells to deliver cytokines to breast cancer, melanoma and pancreatic cancer in rodent models. His laboratory is currently testing these and other tumor-homing cells for delivery of therapeutic nanoparticles, including superparamagnetic core/shell iron/iron oxide nanoparticles for magnetic hyperthermia and imaging in collaboration with Dr. Stefan Bossmann at KSU. In addition, the Bossmann/Troyer labs are developing a protease sensor nanoplatform for early cancer detection.

http://themercury.com/articles/researchers-at-k-state-develop-blood-test-for-cancer-detection
http://www.youtube.com/watch?v=O8elee5Hv4U
http://www.nsf.gov/discoveries/disc_videos.jsp?cntn_id=130241&media_id=75649&org=NSF http://www.nsf.gov/discoveries/disc_summ.jsp?cntn_id=130241
http://themercury.com/articles/k-state-receives-1.3-million-for-cancer-fighting-tool

Selected Publications

Wendel S, Menon S, Alshetaiwi H, Shrestha T, Chlebanowski L, Hsu W, Bossmann S, Narayanan S, Troyer D: Cell based drug delivery: Micrococcus luteus loaded neutrophils as chlorhexidine delivery vehicles in a mouse model of lever abscesses in cattle. PLoS ONE, 10(5): e0128144, 2015.

Wang H, Shrestha T, Basel M, Pyle M, Toledo Y, Konecny A, Thapa P, Ikenberry M, Hohn K, Chikan V, Troyer D, Bossmann S: Hexagnonal Magnetite Nanoprisms: Preparation, characterization, and cellular uptake. Journal of Material Chemistry B, 3: 4647, 2015

Ohta N, Ishiguro S, Kawabata A, Uppalapati D, Pyle M, Troyer D, De S, Zhang Y, Becker K, Tamura M: Human umbilical cord matrix mesenchymal stem cells suppress the growth of breast cancer by expression of tumor suppressor genes. PLoS ONE 10(5):e0123756, 2015

Wang H, Udukala D, Samarakoon T, Basel M, Abayaweera G, Manawadu H, Malalasekera A, Robinson C, Villanueva D, Maynez P, Bossmann L, Riedy E, Barriga J, Wang N, Higgins D, Zhu G, Troyer D, Bossmann S: Synthesis and calibration of magnetic-nanoparticle (Fe/Fe3O4)-based nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases. Photochemical and Photobiological Sciences, 13(2):231-240, 2014

Kawabata A, Ohta N, Seiler G, Seo G-M, Pyle MP, Zhang Y, Becker KG, Troyer D, Tamura M: Naïve rat umbilical cord matrix stem cells significantly attenuate mammary tumor growth through modulation of endogenous immune responses. Cytotherapy, 15:586-597, 2013

Shrestha T, Kalita M, Pokhrel M, Liu Y, Troyer D, Turro C, Bossmann S, Durr H: Maleimide-functionalized photochromic spirodihydroindolizines. The Journal of Organic Chemistry. 78(5):1903-1909, 2013

Bossmann SH, Troyer DL: Point-of-care routine rapid screening: the future of cancer diagnosis? Expert Rev. Mol. Diagn. 13(2):107-109, 2013

Alshetaiwi H, Balivada S, Shrestha T, Pyle M, Basel M, Bossmann S, Troyer D: Luminol-based bioluminescence imaging of mouse mammary tumors. Journal of Photochemistry and Photobiology B: Biology 127:223-228, 2013.

Kalita M, Balivada S, Swarup V, Mencio C, Raman K, Desai R, Troyer D, Balagurunathan K: A nanosensor for ultrasensitive detection of oversulfated chondroitin sulfate contaminant of heparin. Journal of the American Chemical Society 136(2):554-557, 2013

Kwabata A, Ohta N, Seiler G, Pyle M, Ishiguro S, Zhang YQ, Becker KG, Troyer D, Tamura M: Naïve rat umbilical cord matrix stem cells significantly attenuate mammary tumor growth through modulation of endogenous immune responses. Cytotherapy 15(5):586-597, 2013.

Miller D, Rathbun T, Boyle D, Troyer D, Davis D.L.: Confocal Imaging of Trans-epithelial Trafficking by Immune and Umbilical Cord Stem Cells in the Neonatal Porcine Intestine. Anatomia Histologica Embryologica 41(6):461-468, 2012

Basel M, Balivada S, Wang H, Shrestha T, Seo G, Pyle M, Abayaweera G, Daini R, Koper O, Tamura M, Chikan V, Bossmann S, Troyer D: Cell-delivered magnetic nanoparticles causes hyperthermia-mediated increased survival in a murine pancreatic cancer model. International. Journal of Nanomedicine, 7:297-306, 2012

Wang L, Liu Z, Balivada S, Shreshtha T, Bossmann S, Pyle M, Pappan L, Shi J, Troyer D: IL-1beta and TGF beta cooperate to induce neurosphere formation and increase tumorigenicity of adherent LN-229 glioma cells. Stem Cell Research and Therapy, 35, 2012

Seo G-M, Rachakatla R, Balivada S, Pyle M, Shrestha T, Wang H, Tamura M, Bossmann S, Troyer DL: A self-contained enzyme activating prodrug cytotherapy for preclinical melanoma. Mol Biol Reports 39(1):157-165, 2011

UppalapatiD, OhtaN, ZhangY, Kawabata A, PyleMP, BeckerKG, Troyer D,Tamura M: Identification and characterization of unique tumoricidal genes in rat umbilical cord matrix stem cells. Mol Pharm. 8:1549-1558, 2011

Ganta C, Chiyo D, Ayuzawa R, Rachakatla R, Pyle M, Andrews G, Weiss M, Tamura M, Troyer D: Rat umbilical cord stem cells completely abolish rat mammary carcinomas with no evidence of metastasis or recurrence 100 days post-tumor cell inoculation. Cancer Res 69(5): 1815-1820, 2009

Ayuzawa R, Doi C, Rachakatla R, Pyle M, Maurya D, Troyer D, Tamura M: Naïve human umbilical cord matrix derived stem cells significantly attenuate growth of human cancer cells in vitro and in vivo. Cancer Lett, 280:31-37, 2009

Weiss M, Anderson C, Medicetty S, Seshareddy K, Weiss R, VanderWerff I, Troyer D, McIntosh K: Immune properties of human umbilical cord Wharton’s Jelly-Derived Cells. Stem Cells Express 26(11):2865-2874, 2008

Troyer D, Weiss M: Wharton’s Jelly-derived cells are a primitive stromal cell population. Stem Cells 26(3):591-599, 2008