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College of Veterinary Medicine

Xuming Liu

 Research Assistant Professor
Molecular Research and Development
Kansas State Veterinary Diagnostic Laboratory

Ph.D. (2001), Kansas State University, Manhattan, KS, U.S.A.    
M. S. (1989), China Agricultural University, Beijing, China
B. S. (1984), Hunan Agricultural University, Hunan, China  

Phone: (785) 532-3407 (O), 532-5268 (Lab)
Email: xmliu@vet.ksu.edu

Molecular Research and Diagnostic Service

My interests and responsibilities are to develop, validate and improve molecular diagnostic assays for the identification, differentiation, quantification and characterization of animal, zoonotic and foodborne viral and bacterial pathogens using PCR, sequencing and other technologies. We are especially interested in exploring new molecular technologies (e.g. digital PCR, MassTag/LC-MS, microarray, Luminex, and so on) that can be potentially used to increase multiplexing and throughput capabilities, and to lower the cost and to reduce turnaround time for diagnostic services.  In recent years, in addition to the routine diagnostic work, I have emphasized great efforts on research and development of new assays for molecular diagnosis of zoonotic and foodborne pathogens, such as: single cell-based digital PCR detection and association of Shiga toxin-producing Escherichia coli serogroups and major virulence genes,  multiplex molecular serotyping of Salmonella using Luminex xMAP assay and Check&Trace kit, multiplex real-time RT-PCR assay for simultaneous detection and differentiation of influenza A, B, C, and D viruses, as well as antibody detection assay for swine influenza B, C, and D viruses. 

Selected Publications (*Correspondence author)

PubMed Search for Dr. Liu

Xuming Liu, Lance Noll, Xiaorong Shi, Elizabeth Porter, Yin Wang, Colin Stoy, Nanyan Lu, T. G. Nagaraja, Gary Anderson, and Jianfa Bai. 2020. Single cell-based digital PCR detection and association of Shiga toxin-producing Escherichia coli serogroups and major virulence genes. Journal of Clinical Microbiology. 2020 Feb 24. DOI:  https://doi.org/10.1128/JCM.01684-19, https://jcm.asm.org/content/58/3/e01684-19.

Wang Y, Das A, Zheng W, Porter E, Xu L, Noll L, Liu X, Dodd K, Jia W, Bai J. 2019. Development and evaluation of multiplex real-time RT-PCR assays for the detection and differentiation of foot-and-mouth disease virus and Seneca Valley virus 1. Transbound Emerg Dis. 2019 Sep 24. doi: https://doi.org/10.1111/tbed.13373

Zhang, H., Wang, Y., Porter, E., Lu, N., Li, Y., Yuan, F., Lohman, M., Noll, L., Zheng, W., Stoy, C., Lang, Y., Huber, V. C., Ma, W., Peddireddi, L., Fang, Y., Shi, J., Anderson, G., Liu, X*., & Bai, J*. (2019). Development of a multiplex real-time RT-PCR assay for simultaneous detection and differentiation of influenza A, B, C, and D viruses. Diagn Microbiol Infect Dis, 95(1), 59-66.

Wang, Y., Feng, Y., Zheng, W., Noll, L., Porter, E., Potter, M., Cino, G., Peddireddi, L., Liu, X., Anderson, G., & Bai, J. (2019). A multiplex real-time PCR assay for the detection and differentiation of the newly emerged porcine circovirus type 3 and continuously evolving type 2 strains in the United States. J Virol Methods, 269, 7-12.

Zheng, W., Porter, E., Noll, L., Stoy, C., Lu, N., Wang, Y., Liu, X., Purvis, T., Peddireddi, L., Lubbers, B., Hanzlicek, G., Henningson, J., Liu, Z., & Bai, J. (2019). A multiplex real-time PCR assay for the detection and differentiation of five bovine pinkeye pathogens. J Microbiol Methods, 160, 87-92.

Zhang, H., Porter, E., Lohman, M., Lu, N., Peddireddi, L., Hanzlicek, G., Marthaler, D., Liu, X*., & Bai, J*. (2018). Influenza C Virus in Cattle with Respiratory Disease, United States, 2016-2018. Emerg Infect Dis, 24(10), 1926-1929.

Zhang, H., Porter, E. P., Lohman, M., Lu, N., Peddireddi, L., Hanzlicek, G., Marthaler, D., Liu, X*., & Bai, J*. (2018). Complete Genome Sequence of an Influenza C Virus Strain Identified from a Sick Calf in the United States. Microbiol Resour Announc, 7(1).

Noll, L. W., Chall, R., Shridhar, P. B., Liu, X., Bai, J., Delannoy, S., Fach, P., & Nagaraja, T. G. (2018). Validation and Application of a Real-Time PCR Assay Based on the CRISPR Array for Serotype-Specific Detection and Quantification of Enterohemorrhagic Escherichia coli O157:H7 in Cattle Feces(dagger). J Food Prot, 81(7), 1157-1164.

Yang, Y., Wang, Y., Poulsen, E., Ransburgh, R., Liu, X., An, B., Lu, N., Anderson, G., Wang, C., & Bai, J. (2017). Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay. Sci Rep, 7(1), 1067.

Fowler, V. L., Ransburgh, R. H., Poulsen, E. G., Wadsworth, J., King, D. P., Mioulet, V., Knowles, N. J., Williamson, S., Liu, X., Anderson, G. A., Fang, Y., & Bai, J. (2017). Development of a novel real-time RT-PCR assay to detect Seneca Valley virus-1 associated with emerging cases of vesicular disease in pigs. J Virol Methods, 239, 34-37.

Shi, X**., Liu, X**., Wang, Q., Das, A., Ma, G., Xu, L., Sun, Q., Peddireddi, L., Jia, W., Liu, Y., Anderson, G., Bai, J., & Shi, J. (2016). A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses. J Virol Methods, 236, 258-265. (**equal contribution)

Liu, X., Zhang, S., Whitworth, R. J., Stuart, J. J., & Chen, M. S. (2015). Unbalanced activation of glutathione metabolic pathways suggests potential involvement in plant defense against the gall midge Mayetiola destructor in wheat. Sci Rep, 5, 8092.

Liu, X., Khajuria, C., Li, J., Trick, H. N., Huang, L., Gill, B. S., Reeck, G. R., Antony, G., White, F. F., & Chen, M. S. (2013). Wheat Mds-1 encodes a heat-shock protein and governs susceptibility towards the Hessian fly gall midge. Nat Commun, 4, 2070.

Liu, X., Williams, C. E., Nemacheck, J. A., Wang, H., Subramanyam, S., Zheng, C., & Chen, M. S. (2010). Reactive oxygen species are involved in plant defense against a gall midge. Plant Physiol, 152(2), 985-999.

Manuscripts in preparation

  1. Xuming Liu, Hewei Zhang, Xiaorong Shi, Yin Wang, Russell Ransburgh, Elizabeth Porter, Lance Noll, Nanyan Lu, Vijai Pasupuleti, Aneta Karczmarek, T. G. Nagaraja, Gary Anderson, Jianfa Bai. 2019. Comparison of serological method with two molecular methods in serotyping Salmonella strains (in preparation).
  2. Junsheng Dong, Qinghong Ran, Jinping Fu, Nanyan Lu, Elizabeth Porter, Rachel Palinski, Lalitha Peddireddi, Xuming Liu*, Jianfa Bai*. 2019. Whole genome sequence analysis of seven new influenza D virus strains identified from sick cattle in the United States (in preparation). 

Recent Grants

  1. PI: Swine Health Information Center-#17-203 SHIC, $106,100. Development of antibody detection assays for swine influenza B, C, and D viruses. 2018-2019.
  2. Co-PI: Swine Health Information Center-#17-204 SHIC, $67,200. Development and evaluation of antibody detection assay for PCV3 viruses. 2018-2019.
  3. PI: Swine Health Information Center-#16-255 SHIC, $58,500. Multiplex real-time RT-PCR assay for simultaneous detection and differentiation of swine influenza C, D, and B viruses. 2016-2017.
  4. Co-PI: Swine Health Information Center-#16-272 SHIC, $65,700. Detection and differentiation of Seneca Valley virus (SVV) from foot-and-mouth disease virus (FMDV)". 2016-2017.
  5. Co-PI: Swine Health Information Center-#16-257 SHIC, $56,700. Detection and differentiation of PCV3 from PCV2a, PCV2b and the highly prevalent PCV2d mutant strains. 2016-2017.
  6. Co-PI: Swine Health Information Center-#16-256 SHIC, $55,267. Development of sensitive and reliable diagnostic assay to detect atypical porcine pestivirus (APPV) in swine. 2016-2017.
  7. Co-PI: Swine Health Information Center-#16-254 SHIC, $56,500. Development of a TaqMan quantitative RT-PCR test for porcine parainfluenza virus 1. 2016-2017.
  8. Co-PI: National Pork Board, $79,080.  Detection and differentiation of field strains and commonly used vaccine strains of Type 2 PRRS virus in the US.  2016-2017.
  9. Co-PI: Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), $75,000 ($60,000 from CEEZAD, $15,000 matching).  Co-detection of different genotypes of FMDv, CSFv and ASFv with a high-throughput MassTag system.  2015-2016.
  10. Co-PI (PI for Kansas State University subcontract): USDA AFRI Project, $453,658. (KSU portion: $139,364, Subcontract from Purdue University). Insect Effectors and Wheat Resistance Genes. 2011-2014.
  11. Co-PI: USDA-NRI Project, $129,728 (Subcontract from Purdue University). Molecular Genetic Interactions of Wheat Resistance and Hessian Fly Avirulence. 2009-2012.