Training / Capacity Building Capabilities

Research Areas:

1. Antimicrobial Resistance

1. 1. Bacterial isolation and identification – Aerobic, Anaerobic, Microaerophillic and Facultative bacteria.
   2. Phenotypic characterization – strain and or isolate identification by traditional biochemical tests, API 20, RapID ANA etc.
   3. Antimicrobial susceptibility testing – Macrodilution, Agar well diffusion, Disk diffusion, Microdilution, Sensititer assay using NARMS antibiotic plates.
   4. Genotypic characterization – PCR (RT-PCR, qPCR, q RT-PCR, Touchdown PCR, Endpoint PCR), virulence genes detection by PCR, development of PCR assay for disease diagnosis and surveillance.
   5. Whole Genome Sequencing (WGS) – Bacterial WGS to detect AMR and virulence genes.

2. Antibiotic Alternatives

1. 1. Phytophenols – testing plant-based products for their antimicrobial properties
   2. Bacterial secreted products / metabolites – lactic acid producing bacteria
   3. Essential oils
   4. Heavy metals – Copper and Zinc
   5. Cannabinoids
   6. Organic acids
   7. Medium chain fatty acids
   8. Formaldehyde
   9. Complete product testing and antibiotic discovery
   10. Quality control

3. Liver abscess

1. 1. Disease prevalence
   2. Development of novel alternatives to antibiotics for their prevention and control
   3. In-vitro rumen fermentation assays
   4. Development of challenge models to study disease pathogenesis
      1. Fusobacterium necrophorum
      2. Trueperella pyogenes
      3. Salmonella Lubbock

4. BRD research

5. Pre-harvest food safety

1. 1. Escherichia coli – Shiga-toxin producing coli
   2. Enteric colibacillosis – Enterotoxigenic coli
   3. Salmonella
   4. Campylobacter

6. Next-Generation Sequencing

1. 1. Microbiome
   2. Metagenomics (Targeted and Shot-gun)
   3. Metabolomics
   4. Whole genome sequencing
   5. Bioinformatics analyses

Capacity Building

1. 1. Grant writing – funding agencies
   2. Preparation of manuscripts
   3. Training the trainers – future scientists in the area of One Health, Microbiology, Epidemiology, Antimicrobial Resistance
   4. Invited Lectures
   5. Hands-on-training