Post-doc, Pharmacology, University of North Carolina
PhD, Biochemistry/Biophysics, Johns Hopkins
Undergraduate researcher sought
The DeLong biomedical nanotechnology lab in the School of Veterinary Medicine at K-State is currently inviting applications for an undergraduate researcher. We seek a diligent, organized and self-motivated student who can dedicate a minimum of 4-6 hours/week for the next couple of semesters. Ideally, the student would be an advanced sophomore or junior who has had several semesters of laboratory class experience and, once trained, has an interest in progressing their own undergraduate project into their junior/senior years. The lab has three primary areas of research interest: 1) binding, stabilization and delivery of experimental nucleic acid therapeutics, anticancer peptides and CRISPR/CAS9 by nanoparticles, 2) biochemical and immunological activity of nanoparticles and nanobio conjugates and 3) physiometacomposite materials as fluorescent or bioluminescence-based imaging agents. Interested students please send their resume/CV and a written paragraph of interest c/o Dr. Rob DeLong (firstname.lastname@example.org).
Dr. Robert K. DeLong serves as associate professor in the Nanotechnology Innovation Center Kansas State within the Department of Anatomy and Physiology. The group studies the interaction, stabilization and delivery of nucleic acid therapeutics and proteins by nanoparticles, with a particular interest in their anti-cancer, biochemical and immunological activity. Dr. DeLong’s bionanotechnology and nanomedicine work began in industry, returning to academia he helped start-up the Journal of Biomedical Nanotechnology in 2005-2006 and continues to serve on its executive editorial board. Research has received support from NIH-NCI or NIBIB, NSF-DMR, HHMI, the American Physiology Society and the Johnson Cancer Research Center. He has 14 patents or patent applications in the US, Europe and other countries, 1 textbook published by Academic Press/Elsevier on biomolecular interactions for the molecular biology and biochemistry laboratory, and has published numerous book chapters and review articles on RNA and enzyme nanoparticles, their characterization and delivery. Some publications highlighting the group’s work are listed below:
Selected Publications (students underlined)
Comparative functional dynamics studies on the enzyme nano-bio interface. Thomas SE, Comer J, Kim MJ, Marroquin S, Murthy V, Ramani M, Hopke TG, McCall J, Choi SO, DeLong RK. Int J Nanomedicine. 2018 Aug 8;13:4523-4536. doi: 10.2147/IJN.S152222. eCollection 2018. PMID: 30127604
Ramani M, Mudge MC, Morris RT, Zhang Y, Warcholek SA, Hurst MN, Riviere JE, DeLong RK. Zinc Oxide Nanoparticle-Poly I:C RNA Complexes: Implication as Therapeutics against Experimental Melanoma. Mol Pharm. 2017 Mar 6;14(3):614-625. doi: 10.1021/acs.molpharmaceut.6b00795. Epub 2017 Feb 14. PMID: 28135100
Murthy, V, DeLong RK. Engineering the RNA-Nanobio Interface. Bioengineering 2017, 4(1), 13.
DeLong RK, Curtis CB. Toward RNA nanoparticle vaccines: synergizing RNA and inorganic nanoparticles to achieve immunopotentiation. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2017 Mar;9(2). doi: 10.1002/wnan.1415. Epub 2016 Jun 16. Review. PMID: 27312869
Hurst MN, DeLong RK. Two-Dimensional Fluorescence Difference Spectroscopy to Characterize Nanoparticles and their Interactions. Sci Rep. 2016 Sep 14;6:33287. doi: 10.1038/srep33287. PMID: 27624316
Dean JM, DeLong RK. A high-throughput screening assay for the functional delivery of splice-switching oligonucleotides in human melanoma cells. Methods Mol Biol. 2015;1297:187-96. doi: 10.1007/978-1-4939-2562-9_13. PMID:25896004
Bhaumik A, Shearin AM, Delong R, Wanekaya A, Ghosh K. Probing the Interaction at the Nano-Bio Interface Using Raman Spectroscopy: ZnO Nanoparticles and Adenosine Triphosphate Biomolecules. J Phys Chem C: Nanomater Interfaces. 2014 Aug 14;118(32):18631-18639. Epub 2014 Jul 29. PMID:25152799
Reyes-Reveles J, Sedaghat-Herati R, Gilley DR, Schaeffer AM, Ghosh KC, Greene TD, Gann HE, Dowler WA, Kramer S, Dean JM, Delong RK. mPEG-PAMAM-G4 nucleic acid nanocomplexes: enhanced stability, RNase protection, and activity of splice switching oligomer and poly I:C RNA. Biomacromolecules. 2013 Nov 11;14(11):4108-15. doi: 10.1021/bm4012425. Epub 2013 Oct 28. PMID:24164501
Delong RK, Risor A, Kanomata M, Laymon A, Jones B, Zimmerman SD, Williams J, Witkowski C, Warner M, Ruff M, Garrad R, Fallon JK, Hickey AJ, Sedaghat-Herati R. Characterization of biomolecular nanoconjugates by high-throughput delivery and spectroscopic difference. Nanomedicine (Lond). 2012 Dec;7(12):1851-62. doi: 10.2217/nnm.12.70. Epub 2012 Sep 3. PMID:22943129
Parker-Esquivel B, Flores KJ, Louiselle D, Craig M, Dong L, Garrad R, Ghosh K, Wanekaya A, Glaspell G, DeLong RK. Association of poly I:C RNA and plasmid DNA onto MnO nanorods mediated by PAMAM. Langmuir. 2012 Feb 28;28(8):3860-70. doi: 10.1021/la203998r. Epub 2012 Feb 14. PMID:22220841
Functionalized gold nanoparticles for the binding, stabilization, and delivery of therapeutic DNA, RNA, and other biological macromolecules. Delong RK, Reynolds CM, Malcolm Y, Schaeffer A, Severs T, Wanekaya A. Nanotechnol Sci Appl. 2010 Sep 20;3:53-63. doi: 10.2147/NSA.S8984. Review.
Characterization and performance of nucleic acid nanoparticles combined with protamine and gold. DeLong RK, Akhtar U, Sallee M, Parker B, Barber S, Zhang J, Craig M, Garrad R, Hickey AJ, Engstrom E. Biomaterials. 2009 Nov;30(32):6451-9.
Structural characterization and biological evaluation of small interfering RNAs containing cyclohexenyl nucleosides. Nauwelaerts K, Fisher M, Froeyen M, Lescrinier E, Aerschot AV, Xu D, DeLong R, Kang H, Juliano RL, Herdewijn P. J Am Chem Soc. 2007 Aug 1;129(30):9340-8. Epub 2007 Jul 6. PMID:17616127
Tat-conjugated PAMAM dendrimers as delivery agents for antisense and siRNA oligonucleotides. Kang H, DeLong R, Fisher MH, Juliano RL. Pharm Res. 2005 Dec;22(12):2099-106. Epub 2005 Oct 1. PMID:16184444