Research Assistant Professor, Molecular Diagnostics
Ph.D. (2001), Kansas State University, Manhattan, KS, U.S.A.
M. S. (1989), China Agricultural University, Beijing, China
B. S. (1984), Hunan Agricultural University, Hunan, China
Veterinary Diagnostic Laboratory
Department of Diagnostic Medicine/Pathobiology
Kansas State University
2005 Research Park Cir.
Manhattan, KS 66502
Phone: (785) 532-3407 (O), 532-5268 (Lab)
Service and Service-Oriented Research
- My interests and responsibilities on the current position are mainly involved in molecular clinical diagnostic services and service-oriented researches, which include but not limited to routine molecular diagnostic services as well as new molecular diagnostic assay development, validation, and improvement by deploying advanced new technologies for higher throughput molecular diagnostic applications.
- Diagnostic assays based on DNA sequencing, regular PCR, multiplex PCR (mPCR), real-time PCR (qPCR), multiplex qPCR (mqPCR), RT-qPCR, RT-mqPCR, Digital PCR, MassTag/LC-MS, and Luminex have been developed or under development in collaboration with colleagues for highly multiplexed, quicker, and more accurate identifications and quantifications of animal, zoonotic and foodborne viral and bacterial pathogens.
- My knowledge, skills and experience in microbiology, pathology, molecular biology, genetics, genomics and bioinformatics that I acquired from my education and previous work have been directly applied to molecular diagnostic service and new molecular diagnostic assay development.
Liu, X. M., S. Z. Zhang, R. J. Whitworth, J. J. Stuart, M. S. Chen. 2015. Unbalanced activation of glutathione metabolic pathways suggests potential involvement in plant defense against the gall midge Mayetiola destructor. Nature Scientific Reports. 5: 8092 (doi:10.1038/srep08092).
Zhao, Y., (34 other coauthors before me, in alphabetical order by last name), X.M. Liu, (32 coauthor after me). 2015. A massive expansion of effector genes underlies gall-formation in the wheat pest Mayetiola destructor. Current Biology. 25:1-8 (doi:10.1016/j.cub.2014.12.057).
Liu, X. M., C. Khajuria, J. R. Li, H. N. Trick, L. Huang, B. S. Gill, G. R. Reeck, G. Antony, F. F. White, and M.S. Chen. 2013. Wheat Mds-1 encodes a heat-shock protein and governs susceptibility towards the Hessian fly gall midge. Nature Communications.4: 2070 (doi:10.1038/ncomms3070).
Khajuria, C., H. Wang, X. M. Liu, S. Wheeler, J. C. Reese, M. E. Bohssini, R. J. Whitworth, M. S. Chen. 2013. Mobilization of lipids and fortification of cell wall and cuticle are important in host defense against Hessian fly. BMC Genomics. 14:423 (doi:10.1186/1471-2164-14-423).
Chen, M. S., X. M. Liu, Z. H. Yang, H. X. Zhao, R. H Shukle, J. J Stuart, and S. Hulbert. 2010. Unusual conservation among genes encoding small secreted salivary gland proteins from a gall midge. BMC Evolutionary Biology 2010, 10: 296 (doi:10.1186/1471-2148-10-296).
Liu, X. M., C. E. Williams, J. A. Nemacheck, H. Wang,S. Subramanyam, C. Zheng,and M. S. Chen. 2010. Reactive oxygen species are involved in plant defense against a gall midge. Plant Physiology.152:985-999.
Smith,C. M., X. M. Liu, L. J. Wang, X. Liu, M. S. Chen, S. Starkey, and J. F. Bai. 2010. Aphid feeding activates expression of a transcriptome of oxylipin-based defense signals in wheat involved in resistance to herbivory. Journal of Chemical Ecology. 36: 260-276.
Liu, X. M., J. F. Bai, L. Huang, L. C. Zhu, X. Liu, N. Y. Weng, J. C. Reese, M. Harris, J. J. Stuart, and M. S. Chen. 2007. Gene expression of different wheat genotypes during attack by virulent and avirulent Hessian fly (Mayetiola destructor) larvae. Journal of Chemical Ecology. 33: 2171-2194.
Maddur, A.A., X. M.Liu, Y. C. Zhu, J.P. Fellers, B. Oppert, Y. S. Park, J. Bai, G. E. Wilde and M.-S. Chen. 2006. Cloning and characterization of protease inhibitor-like cDNAs from the Hessian fly, Mayetiola destructor (Say). Insect Molecular Biology. 15(4): 485-496.
Liu, X. M., G. L. Brown-Guedira, J. H. Hatchett, J. O. Owuoche, and M. S. Chen. 2005. Genetic characterization and molecular mapping of a Hessian fly resistance gene Hdic transferred from T. turgidum ssp. dicoccum to common wheat. Theoretical and Applied Genetics. 111:1308-1315.
Liu, X. M., B. S. Gill, and M. S. Chen. 2005. Hessian fly resistance gene H13 mapped to a distal cluster of R genes in chromosome 6DS of wheat. Theoretical and Applied Genetics. 111:243-249.
Liu, X. M., A. K. Fritz, J. C. Reese, G. E. Wilde, B. S. Gill, and M. S. Chen. 2005. H9, H10, and H11 compose a cluster of Hessian fly-resistance genes in the distal gene-rich region of wheat chromosome 1AS. Theoretical and Applied Genetics. 110:1473-1480.
Liu, X. M., C. M. Smith, B. R. Friebe, and B. S. Gill. 2005. Molecular mapping and allelic relationships of Russian wheat aphid resistance genes. Crop Science. 45: 2273-2280.
Liu, X. M., J. P. Fellers, G. E. Wilde, J. J. Stuart, and M.S. Chen. 2004. Characterization of two genes expressed in the salivary glands of the Hessian fly [Mayetiola destructor (Say)]. Insect Biochemistry and Molecular Biology. 34(3):229-237.
Chen, M. S., J. P. Fellers, J. J. Stuart, J. C. Reese, and X. M. Liu. 2004. A group of related cDNAs encoding secreted proteins from Hessian fly [Mayetiola destructor (Say)] salivary glands. Insect Molecular Biology. 13(1):101-108.
Liu, X. M., C. M. Smith, and B. S. Gill. 2002. Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6. Theoretical and Applied Genetics. 104: 1042-1048.
Liu, X. M., C. M. Smith, B. S. Gill, and V. Tolmay. 2001. Microsatellite markers linked to six Russian wheat aphid resistance genes in wheat. Theoretical and Applied Genetics. 102: 504-510.
Liu,X. M. and D. S. Jin. 1998. A new biotype (CHN-1) of greenbug, Schizaphis graminum, identified in Beijing. Acta Entomologia Sinica. 41(2): 141-144.