Total mRNA is isolated (Qiagen) and quantified by spectrophotometry (Nanodrop). The quality of RNA is evaluated by a microfluidic electrophoresis (BioAnalyzer).
Gene expression is analyzed on a global level using gene chips (Affymetrix) or on an individual level using quantitative RT-PCR (Qiagen). Global gene expression analysis requires amplification and labeling of samples (NuGen).
Quantitative RT-PCR reactions are assembled in the 96-well format by a liquid-handling robot (Beckman-Coulter). PCR reactions are monitored by SYBR green fluorescence (iCycler, BioRad).