Total protein is isolated and quantified by fluorescence spectrophotometry (NanoOrange, Invitrogen). Alternatively, proteins are isolated by immunoprecipitation.
Protein expression is analyzed by Western blotting or confocal immunohistochemistry. Protein expression in Western blots is quantified against actin or tubulin.
Glycoproteins are identified by differences in mobility before and after enzymatic deglycosylation.