Kansas State Veterinary Diagnostic Laboratory expands Tritrichomonas foetus diagnostic capability to include both culture and real-time PCR in a combined assay.
KSVDL has added the real-time PCR assay of McMillen and Lew (Veterinary Parasitology 141 (2006) 204-215) to compliment the InPouch™TF culture system in the diagnosis of Tritrichomonas foetus. Samples for both tests should be submitted in the same InPouch (one pouch/bull) using the sampling directions included with the pouches. Directions are also available online at http://www.biomeddiagnostics.com/docs/INSERT-TF.pdf.
Preputial samples should be immediately inoculated in the InPouch™TF culture system and must be received by the lab within 48 hours after inoculation. Overnight or one-day delivery is the best, or they can be delivered personally to KSVDL receiving. Special arrangements for after hours or Saturday delivery can be made but only if you call ahead (785-532-4619).
The combined assay consisting of the InPouch™TF culture and real-time PCR is being offered at a cost of $35.00 per sample ($7.75 for each culture test plus $27.25 for each real-time PCR test). The culture InPouch™TF will still be available as a separate assay for $ 7.75 per sample. Pouches may be ordered in bulk from BioMed Diagnostics (1-800-964-6466) or by the pouch from KSVDL (1-866-512-5650).