Philine Wangemann Lab
Gene Expression
Total mRNA is isolated (Qiagen) and quantified by
spectrophotometry (Nanodrop). The quality of RNA is evaluated by a
microfluidic electrophoresis (BioAnalyzer).
Gene expression is analyzed on a global level using gene chips (Affymetrix)
or on an individual level using quantitative RT-PCR (Qiagen). Global
gene expression analysis requires amplification and labeling of samples
(NuGen).
Quantitative RT-PCR reactions are assembled in the 96-well format by a
liquid-handling robot (Beckman-Coulter). PCR reactions are monitored by
SYBR green fluorescence (iCycler, BioRad).
Papers from the lab:
Wangemann et al (2004)
Singh et al (2005)
Jabba et al (2006)