Philine Wangemann Lab
Confocal Immunocytochemistry
Immunohistochemistry is
performed on cryosections or on whole-mounts of isolated
tissues. Tissues used in the lab are commonly stria
vascularis and organ of Corti of the inner ear. Preparations
are visualized by confocal microscopy (510 META, Carl Zeiss).
Proteins are labeled with protein-specific primary and Alexa-conjugated
secondary antibodies. Stains for nucleic acids (DAPI) and
for actin filaments (phalloidin) are used to provide
orientation. Laser-scanning bright-field imaging provides
orientation and clues toward the quality of tissue
preservation.
Papers from the lab:
Wangemann et al (2004)
Jabba et al (2006)
Wangemann et al (2007)