Faculty
Deryl Troyer
DVM, Kansas State
University, 1972
PhD, Kansas State University, 1985
Phone (785) 532-4509
e-mail troyer@vet.k-state.edu
Research Interests:
Dr. Troyer’s laboratory is investigating the
properties of early postnatal multipotent stem cells that can be
isolated non-invasively in large numbers from a non-controversial
source. These cells are termed umbilical cord matrix stem (UCMS) cells
to emphasize that they are isolated from the umbilical cord Wharton’s
jelly rather than from cord blood. They are being developed as a source
of replacement cells for somatic cells damaged or lost due to
developmental or degenerative diseases, trauma or aging and their
potential use in targeted delivery of therapeutic peptides or proteins.
The Troyer lab is also investigating the use of these stem cells for
cancer therapy. They are being tested as a platform for targeted
delivery of anti-tumor molecules. Strategies employed to achieve this
goal include cell-mediated gene therapy, delivery of therapeutic
nanoparticles, and delivery of immuno-modulatory drugs. Finally, Dr.
Troyer is investigating the potential of these cells for delivery of
anti-infectious or anti-inflammatory agents.
Dr. Troyer is part of a growing KSU Stem Cell Biology Research Team
comprised of Drs. Mark Weiss (Department of Anatomy and Physiology), and
Duane Davis, Brad Johnson and and David Grieger (Dept. of Animal
Sciences and Industry, and is a Founding Fellow of the
Midwest Institute of Comparative Stem Cell Biology.
Courses Taught:
AP 710 Microscopic Anatomy (Course coordinator)
AP 711 Stem Cells and Comparative Biomedicine
AP 901 Molecular Neurobiology
AP 825 Special Anatomy
Selected publications:
Weiss M,
Troyer D: Stem cells in
the umbilical cord. Stem Cell Reviews 2(2):155-162, 2007.
Rachakatla R, Marini F, Weiss M, Tamura M,
Troyer D: Development
of human umbilical cord matrix stem cell-based gene therapy for
experimental lung tumors. Cancer Gene Therapy 14:828-835,2007.
Rachakatla R, Pyle M, Ayuzawa R, Edwards S, Marini F, Weiss M, Tamura M,
Troyer D:
Combination treatment of human umbilical cord matrix stem cell-based
interferon-beta gene therapy and 5-fluorouracil reduces growth of
metastatic human breast cancer in SCID mouse lungs. Cancer
Investigation, In Press.
Troyer D, Weiss
M: Wharton’s Jelly-derived cells are a primitive stromal cell
population. Invited review, Stem Cells 26(3):591-599, 2008.
Ganta C, Aibin S, Battina S, Pyle M, Rana S, Hua D, Tamura M,
Troyer D: Combination
of nanogel polyethylene glycol-polyethyleneimine and 6 (hydroxymethyl)-1,4-anthracenedione
as an anticancer nanomedicine. Nanoscience and Nanotechnology, In Press.
Kiran S, Troyer D,
Weiss M: Method to isolate mesenchymal-like cells from Wharton’s jelly
of umbilical cord. In: Stem Cell Methods, Methods in Cell Biology
series, JP Mather, ed, Academy Press, In Press.
Carlin C, Davis D, Weiss M, Schultz B,
Troyer D: Expression of Early
transcription factors, Oct-4, Sox-2 and Nanog by porcine umbilical cord
(PUC) matrix cells. Reproductive Biology and Endocrinology 4(1):8,
February 6, 2006.
Weiss ML, Medicetty S, Bledsoe AR, Rachakatla RS, Coi M, Merchav S, Luo
Y, Rao MS, Velagaleti G, Troyer D: Human
umbilical cord matrix stem cells: preliminary characterization and
effect of transplantation in a rodent model of Parkinson’s disease. Stem
Cells 24:781-792, 2006.
Medicetty S, Fahrenholtz C, Bledsoe A, Troyer D,
Mitchell K, Weiss M: Transplantation of pig stem cells into rat brain:
Proliferation during the first 8 weeks. Experimental Neurology
190(1):32-44, 2004.
Weiss ML, Hix JE, Medicetty S, Mitchell KE, Grieger D,
Troyer DL: Xenotransplantation of porcine
umbilical cord matrix cells into the rat. Experimental Neurology
182(2):288-299, 2003.
Mitchell K, Weiss M, Medicetty S, Mitchell B, Martin P, Davis D, Morales
L, Helwig B, Beerenstrauch M, Abou-Easa K, Hildreth T,
Troyer D: Matrix cells from Wharton’s
jelly form neurons and glia, Stem Cells 21(1):61-70, 2003.
Troyer DL, Weiss ML, Mitchell KE, Martin
PL, Davis DL: Incorporation of bovine bone marrow stromal cells into
porcine fetal tissues after xenotransplantation. Anatomia Histologia
Embryologia 32(2):98-101,2003.
Martin PL, Davis D, Weiss M, Grieger D, Abou-Easa K,
Troyer D: Xenotransplantation of bovine
bone marrow stromal cells into fetal pigs: incorporation into skeletal
muscle. Animal Biotechnology 12(2):183-191, 2001.
Zhang G, Hiraiwa H, Yasue H, Wu H, Ross CR,
Troyer D, Blecha F: Cloning and characterization of the gene for
a new epithelial beta-defensin, genomic structure, chromosomal
localization, and evidence for its constituitive expression. Journal of
Biological Chemistry 274:24031-24037, 1999.
Hu J, Troyer DL: Physical assignment of
two porcine microsatellites to the short arm of chromosome 1 by modified
DISC-PCR. Animal Biotechnology 9(1):47-54, 1998.
Zhang S, Troyer D, Kapil S, Zheng L,
Kennedy G, Weiss M, Xue W, Wood C, Minocha H: Detection of Proviral DNA
of Bovine Immunodeficiency Virus in bovine tissues by PCR and PCR-in
situ hybridization. Virology 236:249-257, 1997.
Choi I, Troyer DL, Cornwell DL, Kirby-Dobbels
KR, Collante WR, Simmen FA: Closely related genes encode developmental
and tissue isoforms of porcine cytochrome P450 aromatase. DNA and Cell
Biology 16(6):769-777, 1997.
Alexander LJ, Troyer DL, Rohrer GA, Smith
TPL, Beattie CW: Physical assignments of 68 porcine cosmid and lambda
clones containing polymorphic microsatellites. Mammalian Genome
7:368-372, 1996.