Faculty

 

 

 

 

Deryl Troyer

DVM, Kansas State University, 1972
PhD, Kansas State University, 1985

Phone (785) 532-4509
e-mail troyer@vet.k-state.edu

 

 

 

Research Interests:

Dr. Troyer’s laboratory is investigating the properties of early postnatal multipotent stem cells that can be isolated non-invasively in large numbers from a non-controversial source. These cells are termed umbilical cord matrix stem (UCMS) cells to emphasize that they are isolated from the umbilical cord Wharton’s jelly rather than from cord blood. They are being developed as a source of replacement cells for somatic cells damaged or lost due to developmental or degenerative diseases, trauma or aging and their potential use in targeted delivery of therapeutic peptides or proteins. The Troyer lab is also investigating the use of these stem cells for cancer therapy. They are being tested as a platform for targeted delivery of anti-tumor molecules. Strategies employed to achieve this goal include cell-mediated gene therapy, delivery of therapeutic nanoparticles, and delivery of immuno-modulatory drugs. Finally, Dr. Troyer is investigating the potential of these cells for delivery of anti-infectious or anti-inflammatory agents.

Dr. Troyer is part of a growing KSU Stem Cell Biology Research Team comprised of Drs. Mark Weiss (Department of Anatomy and Physiology), and Duane Davis, Brad Johnson and and David Grieger (Dept. of Animal Sciences and Industry, and is a Founding Fellow of the Midwest Institute of Comparative Stem Cell Biology.
 

Courses Taught:

AP 710 Microscopic Anatomy (Course coordinator)
AP 711 Stem Cells and Comparative Biomedicine
AP 901 Molecular Neurobiology
AP 825 Special Anatomy

Selected publications:

Weiss M, Troyer D: Stem cells in the umbilical cord. Stem Cell Reviews 2(2):155-162, 2007.

Rachakatla R, Marini F, Weiss M, Tamura M, Troyer D: Development of human umbilical cord matrix stem cell-based gene therapy for experimental lung tumors. Cancer Gene Therapy 14:828-835,2007.

Rachakatla R, Pyle M, Ayuzawa R, Edwards S, Marini F, Weiss M, Tamura M, Troyer D: Combination treatment of human umbilical cord matrix stem cell-based interferon-beta gene therapy and 5-fluorouracil reduces growth of metastatic human breast cancer in SCID mouse lungs. Cancer Investigation, In Press.

Troyer D, Weiss M: Wharton’s Jelly-derived cells are a primitive stromal cell population. Invited review, Stem Cells 26(3):591-599, 2008.

Ganta C, Aibin S, Battina S, Pyle M, Rana S, Hua D, Tamura M, Troyer D: Combination of nanogel polyethylene glycol-polyethyleneimine and 6 (hydroxymethyl)-1,4-anthracenedione as an anticancer nanomedicine. Nanoscience and Nanotechnology, In Press.

Kiran S, Troyer D, Weiss M: Method to isolate mesenchymal-like cells from Wharton’s jelly of umbilical cord. In: Stem Cell Methods, Methods in Cell Biology series, JP Mather, ed, Academy Press, In Press.

Carlin C, Davis D, Weiss M, Schultz B, Troyer D: Expression of Early transcription factors, Oct-4, Sox-2 and Nanog by porcine umbilical cord (PUC) matrix cells. Reproductive Biology and Endocrinology 4(1):8, February 6, 2006.

Weiss ML, Medicetty S, Bledsoe AR, Rachakatla RS, Coi M, Merchav S, Luo Y, Rao MS, Velagaleti G, Troyer D: Human umbilical cord matrix stem cells: preliminary characterization and effect of transplantation in a rodent model of Parkinson’s disease. Stem Cells 24:781-792, 2006.

Medicetty S, Fahrenholtz C, Bledsoe A, Troyer D, Mitchell K, Weiss M: Transplantation of pig stem cells into rat brain: Proliferation during the first 8 weeks. Experimental Neurology 190(1):32-44, 2004.

Weiss ML, Hix JE, Medicetty S, Mitchell KE, Grieger D, Troyer DL: Xenotransplantation of porcine umbilical cord matrix cells into the rat. Experimental Neurology 182(2):288-299, 2003.

Mitchell K, Weiss M, Medicetty S, Mitchell B, Martin P, Davis D, Morales L, Helwig B, Beerenstrauch M, Abou-Easa K, Hildreth T, Troyer D: Matrix cells from Wharton’s jelly form neurons and glia, Stem Cells 21(1):61-70, 2003.

Troyer DL, Weiss ML, Mitchell KE, Martin PL, Davis DL: Incorporation of bovine bone marrow stromal cells into porcine fetal tissues after xenotransplantation. Anatomia Histologia Embryologia 32(2):98-101,2003.

Martin PL, Davis D, Weiss M, Grieger D, Abou-Easa K, Troyer D: Xenotransplantation of bovine bone marrow stromal cells into fetal pigs: incorporation into skeletal muscle. Animal Biotechnology 12(2):183-191, 2001.

Zhang G, Hiraiwa H, Yasue H, Wu H, Ross CR, Troyer D, Blecha F: Cloning and characterization of the gene for a new epithelial beta-defensin, genomic structure, chromosomal localization, and evidence for its constituitive expression. Journal of Biological Chemistry 274:24031-24037, 1999.

Hu J, Troyer DL: Physical assignment of two porcine microsatellites to the short arm of chromosome 1 by modified DISC-PCR. Animal Biotechnology 9(1):47-54, 1998.

Zhang S, Troyer D, Kapil S, Zheng L, Kennedy G, Weiss M, Xue W, Wood C, Minocha H: Detection of Proviral DNA of Bovine Immunodeficiency Virus in bovine tissues by PCR and PCR-in situ hybridization. Virology 236:249-257, 1997.

Choi I, Troyer DL, Cornwell DL, Kirby-Dobbels KR, Collante WR, Simmen FA: Closely related genes encode developmental and tissue isoforms of porcine cytochrome P450 aromatase. DNA and Cell Biology 16(6):769-777, 1997.

Alexander LJ, Troyer DL, Rohrer GA, Smith TPL, Beattie CW: Physical assignments of 68 porcine cosmid and lambda clones containing polymorphic microsatellites. Mammalian Genome 7:368-372, 1996.