DVM, Kansas State
PhD, Kansas State University, 1985
Phone (785) 532-4509
The emphasis of my laboratory is in stem cell and cancer biology, particularly in the utilization of stem cells as delivery vehicles for targeted cancer therapy. We have discovered a novel and potentially inexhaustible source of stem cells isolated from the matrix of umbilical cords (UCMS cells). We have successfully used engineered UCMS cells to deliver cytokines to breast cancer, melanoma and pancreatic cancer in rodent models. We are currently testing them for delivery of therapeutic molecules in nanoparticles, and superparamagnetic core/shell/shell iron/iron oxide/gold nanoparticles for magnetic hyperthermia and imaging. We have successfully used cell-mediated delivery of magnetic nanoparticles for successful attenuation of subcutaneous melanomas application of an external A/C magnetic field. We are also utilizing defensive cells as delivery vehicles for cancer therapy. In addition, we are developing strategies for early cancer detection.
Basel M, Balivada S, Wang H, Shrestha T, Seo G, Pyle M, Abayaweera G, Daini R, Koper O, Tamura M, Chikan V, Bossmann S, Troyer D: Cell-delivered magnetic nanoparticles causes hyperthermia-mediated increased survival in a murine pancreatic cancer model. International. Journal of Nanomedicine, 7:297-306, 2012; PMID: 22287840; PMCID: PMC3265998.
Wang L, Liu Z, Balivada S, Shreshtha T, Bossmann S, Pyle M, Pappan L, Shi J, Troyer D: IL-1beta and TGF beta cooperate to induce neurosphere formation and increase tumorigenicity of adherent LN-229 glioma cells. Stem Cell Research and Therapy, 35, 2012; PMID: 22330721.
Shrestha T, Seo G, Basel M, Kalita M, Wang H, Villanueva D, Pyle M, Troyer D, Bossmann S: Stem cell-based photodynamic therapy: 1. In-vitro studies. Photochem. Photobiol. Chem. 11(7):1251-1258, 2012 PMID: 22565929
Wang H, Shrestha T, Basel M, Dani R, Seo G-M, Balivada S, Pyle M, Prock H, Koper O, Thapa P, Moore D, Li P, Chikan V, Troyer D, Bossmann S: Magnetic Fe/Fe304 nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages. Beilstein Journal of Nanotechnology 3:444-455, 2012.
Miller D, Rathbun T, Boyle D, Troyer D, Davis D.L.: Confocal Imaging of Trans-epithelial Trafficking by Immune and Umbilical Cord Stem Cells in the Neonatal Porcine Intestine. Anatomia Histologica Embryologica 41(6):461-468, 2012 PMID: 22616894
Kawabata A, Ohta N, Seiler G, Seo G-M, Pyle MP, Zhang Y, Becker KG, Troyer D, Tamura M: Naïve rat umbilical cord matrix stem cells significantly attenuate mammary tumor growth through modulation of endogenous immune responses. Cytotherapy, 15:586-597, 2013
Shrestha T, Kalita M, Pokhrel M, Liu Y, Troyer D, Turro C, Bossmann S, Durr H: Maleimide-functionalized photochromic spirodihydroindolizines. The Journal of Organic Chemistry. 78(5):1903-1909, 2013 PMID: 23095100.
Perera A, Wang M, Megh R, P m, Gamage P, Kalita M, Wendel S, Sears B, Welidenlya D, Liu Y, Turro C, Troyer D, Bossmann S: Channel blocking of MspA revisited. Langmuir 29(1):308-315 PMID: 23214433.
Bossmann SH, Troyer DL: Point-of-care routine rapid screening: the future of cancer diagnosis? Expert Rev. Mol. Diagn. 13(2):107-109, 2013
Perera A, Wang H, Shrestha T, Troyer D, Bossmann S: Nanoscopic surfactant behavior of the porin MspA in aqueous media. Beistein Journal of Nanotechnology. 4:278-284, 2013.
Syed L, Rochford C, Wu J, Richter M, Balivada S, Troyer D, Li J: An ultrasensitive chemiluminescence method for trace blood detection using luminol-labeled gold nanoparticles. Analyst 138:5600-5609, 2013.
Alshetaiwi H, Balivada S, Shrestha T, Pyle M, Basel M, Bossmann S, Troyer D: Luminol-based bioluminescence imaging of mouse mammary tumors. Journal of Photochemistry and Photobiology B: Biology 127:223-228, 2013.
Kalita M, Balivada S, Swarup V, Mencio C, Raman K, Desai R, Troyer D, Balagurunathan K: A nanosensor for ultrasensitive detection of oversulfated chondroitin sulfate contaminant of heparin. Journal of the American Chemical Society 136(2):554-557, 2013
Wang H, Udukala DN, Samarakoon TN, Basel MT, Kalita M, Abayaweera G, Manawadu H, Malalasekera A, Robinson C, Villanueva D, Maynez P, Bossmann L, Riedy E, Barriga J, Wang N, Li P, Higgins DA, Zhu G, Troyer DL, Bossmann SH: Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases. Photochem Photobiol Sci Epub ahead of print, 2013
Kwabata A, Ohta N, Seiler G, Pyle M, Ishiguro S, Zhang YQ, Becker KG, Troyer D, Tamura M: Naïve rat umbilical cord matrix stem cells significantly attenuate mammary tumor growth through modulation of endogenous immune responses. Cytotherapy 15(5):586-597, 2013.