College of Veterinary Medicine

Faculty

Deryl Troyer

DVM, Kansas State University, 1972
PhD, Kansas State University, 1985

Phone (785) 532-4509
e-mail troyer@vet.k-state.edu

Research interests

The emphasis of my laboratory is in stem cell and cancer biology, particularly in the utilization of stem cells as delivery vehicles for targeted cancer therapy. We have discovered a novel and potentially inexhaustible source of stem cells isolated from the matrix of umbilical cords (UCMS cells). We have successfully used engineered UCMS cells to deliver cytokines to breast cancer, melanoma and pancreatic cancer in rodent models. We are currently testing them for delivery of therapeutic molecules in nanoparticles, and superparamagnetic core/shell/shell iron/iron oxide/gold nanoparticles for magnetic hyperthermia and imaging. We have successfully used cell-mediated delivery of magnetic nanoparticles for successful attenuation of subcutaneous melanomas application of an external A/C magnetic field. We are also utilizing defensive cells as delivery vehicles for cancer therapy. In addition, we are developing strategies for early cancer detection.

Selected Publications:

Basel M, Balivada S, Wang H, Shrestha T, Seo G, Pyle M, Abayaweera G, Daini R, Koper O, Tamura M, Chikan V, Bossmann S, Troyer D: Cell-delivered magnetic nanoparticles causes hyperthermia-mediated increased survival in a murine pancreatic cancer model. International. Journal of Nanomedicine, 7:297-306, 2012; PMID: 22287840; PMCID: PMC3265998.

Basel M, Balivada S, Shrestha T, Seo G, Pyle M, Bossmann S, Troyer D: A cell-delivered and activated SN38-dextran prodrug increases survival in a murine disseminated pancreatic cancer model. Small, 8(6):913-920, 2012; PMID: 22238072; PMCID pending.

Wang L, Liu Z, Balivada S, Shreshtha T, Bossmann S, Pyle M, Pappan L, Shi J, Troyer D: IL-1beta and TGF beta cooperate to induce neurosphere formation and increase tumorigenicity of adherent LN-229 glioma cells. Stem Cell Research and Therapy, 35, 2012; PMID: 22330721.

Shrestha T, Seo G, Basel M, Kalita M, Wang H, Villanueva D, Pyle M, Troyer D, Bossmann S: Stem cell-based photodynamic therapy: 1. In-vitro studies. Photochem. Photobiol. Chem. 11(7):1251-1258, 2012 PMID: 22565929

Troyer D, Bossmann S: Utilization of magnetic nanoparticles for cancer therapy. In: Nanomedicine and Cancer, pp 84-105, Editors, Preedy VR and Srirajaskanthan R, CRC Press Science Publishers, Enfield, NH, 2012.

Basel M, Balivada S, Beck A, Kerrigan M, Pyle M, Dekkers J, Wyatt C, Rowland R, Anderson D, Bossmann S, Troyer D: Human xenografts are not rejected in a naturally occurring immunodeficient porcine line: a human tumor model in pigs. BioResearch Open Access, Epub, 2:DOI. 10.1089/biores.2012.9902, 2012; PMID: 23016149; PMCID: PMC3388369.

Wang H, Shrestha T, Basel M, Dani R, Seo G-M, Balivada S, Pyle M, Prock H, Koper O, Thapa P, Moore D, Li P, Chikan V, Troyer D, Bossmann S: Magnetic Fe/Fe304 nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages. Beilstein Journal of Nanotechnology 3:444-455, 2012, PMID: 23016149.

Miller D, Rathbun T, Boyle D, Troyer D, Davis D.L.: Confocal Imaging of Trans-epithelial Trafficking by Immune and Umbilical Cord Stem Cells in the Neonatal Porcine Intestine. Anatomia Histologica Embryologica, 41(6):461-468, 2012 PMID: 22616894.

Shrestha T, Kalita M, Pokhrel M, Liu Y, Troyer D, Turro C, Bossmann S, Durr H: Maleimide-functionalized photochromic spirodihydroindolizines. The Journal of Organic Chemistry. Epub ahead of print, 2012 PMID: 23095100.

Perera A, Wang M, Megh R, P m, Gamage P, Kalita M, Wendel S, Sears B, Welidenlya D, Liu Y, Turro C, Troyer D, Bossmann S: Channel blocking of MspA revisited. Epub ahead of print, Dec. 13, 2012, Langmuir, PMID: 23214433.

Basel MT, Shrestha T, Troyer D, Bossmann SH: Protease-sensitive, polymer-caged liposomes: a method for making highly targeted liposomes using triggered release. ACS Nano, 5(3):2162-2175, 2011, PMID: 21314184; PCMID pending.

Tamura M, Troyer D, Maurya D, Rachakatla R: Cytokines and Lung Cancer, In: Cytokines, pp 173-187, Editors Preedy VR and Hunter RJ, CRC Press, 2011.

Seo G-M, Rachakatla R, Balivada S, Pyle M, Shrestha T, Wang H, Tamura M, Bossmann S, Troyer DL: A self-contained enzyme activating prodrug cytotherapy for preclinical melanoma. Mol Biol Reports 39(1):157-165, 2011; NIHMS304695; PMID: 21567204; PCMID: PMC 3222711.

Uppalapati D, Ohta N, Zhang Y, Kawabata A, Pyle MP, Becker KG, Troyer D, Tamura M: Identification and characterization of unique tumoricidal genes in rat umbilical cord matrix stem cells. Mol Pharm. 8:1549-1558, 2011; PMID: 21851062 ; NIHMS326348; PCMID: PMC3202613.

Tamura M, Kawabata A, Ohta N, Uppalapati K, Becker K, Troyer D: Wharton’s jelly stem cells as agents for cancer therapy. The Open Tissue Engineering and Regenerative Medicine Journal. 4:39-47, 2011

Basel M, Balivada S, Wang H, Shrestha T, Seo G, Pyle M, Abayaweera G, Daini R, Koper O, Tamura M, Chikan V, Bossmann S, Troyer D: Cell-delivered magnetic nanoparticles casuses hyperthermia-mediated increased survival in a murine pancreatic cancer model. International. Journal of Nanomedicine, 7:297-306, 2012; PMID: 22287840; PMCID: PMC3265998.

Maurya D, Doi C, Pyle M, Rachakatla R, Davis D, Tamura M, Troyer D: Non-random tissue distribution of human umbilical cord matrix stem cells after transplant into SCID mice. World Journal of Stem Cells, 3(4): 34-42, 2011, PMCID: PMC3097938.

Maurya D, Ayuzawa R, Doi C, Troyer D, Tamura M: Topoisomerase I inhibitor SN-38 effectively attenuates the growth of non-small cell lung cancer cell lines in vitro and in vivo. Journal of Environmental Pathology Toxicology and Oncology 30(1):1-10, 2011.

Doi C, Maurya D, Pyle M, Troyer D, Tamura M: Cytotherapy with naïve rat umbilical cord matrix stem cells significantly attenuates growth of murine pancreatic cancer cells and increases mouse survival in syngeneic mice. Cytotherapy, 12:408-417. 2010. NIHMSID # 210621, PMCID: PMC2896419.

Matsuzuka T, Rachakatla R, Chiyo D, Maurya D, Ohta N, Kawabata A, Pyle M, Pickel L, Reischman J, Marini F, Troyer D, Tamura M: Human umbilical cord matrx-derived stem cells expressing interferon beta gene significantly attenuate bronchioloalveolar carcinoma xenografts in SCID mice. Lung Cancer 70:28-36, 2010. PMID: 20138387; NIHMS171227.

Doi C, Egashira N, Ayuzawa R, Kawabata A, Maurya D, Ohta N, Upparapati L, ayuzawa R, Pickel L, Isayama Y, Troyer D, Takekoshi S, Tamura M: Angiotensin II type 2receptor signaling significantly attenuates growth of murine pancreatic carcinoma grafs in syngeneic mice. BMC Cancer, Feb 24;10(1):67, 2010, PMCID: PMC2846883.

Maurya D, Doi C, Pyle M, Kawabata A, Troyer D, Tamura M: Therapy with un-engineered naïve rat umbilical cord matrix stem cells markedly inhibits growth of murine lung adenocarcioma. BMC Cancer 10:590, 2010.

Rachakatla R, Troyer D: Wharton’s jelly stromal cells as potential delivery vehicles for cancer therapeutics. Future Oncology 5(8):1237-1244, 2009.

Balivada S, Rahcakatla R, Wang H, Samarakoon T, Dani R, Pyle M, Kroh F, Walker B, Leaym, Koper O, Tamura M, Chikan V, Bossmann S, Troyer D: A/C magnetic hyperthermia of melanoma mediated by iron(0)/iron oxide core/shell magnetic nanoparticles: a mouse study. BMC Cancer, 10:119, 2010, PMCID: PMC2859385.

Rachakatla R, Balivada S, Seo G, Myers C, Wang H, Samarakoon T, Dani R, Pyle M, Kroh F, Walker B, Leaym X, Koper O, Chikan V, Bossmann S, Tamura M, Troyer D: Attenuation of mouse melanoma by A/C magnetic field after delivery of bi-magnetic nanoparticles by neural progenitor cells. ACS Nano 4(12):7093-7104, 2010, PubMed #21058696, NIHMSID#251431, Publ.ID:nn100870z; PMCID# PMC3011034.

Tamura M, Troyer D, Maurya D, Rachakatla R: Cytokines and Lung Cancer, In: Cytokines, pp 173-187, Editors Preedy VR and Hunter RJ, CRC Press, 2011.

Troyer D, Bossmann S: Utilization of magnetic nanoparticles for cancer therapy. Nanomedicine and Cancer, Victor Preedy, ed., Science Publishers, Enfield, NH, 2011.

Seo G-M, Rachatakla R, Balivada S, Pyle M, Shrestha T, Wang H, Tamura M, Bossmann S, Troyer DL: A self-contained enzyme activating prodrug cytotherapy for preclinical melanoma. Mol Biol Rep, Epub ahead of print, 2011; NIHMS304695;PCMID pending.

Reeds K, Higginbotham M, McCaw D, Rachakatla R, Seo G-M, Pyle M, Troyer D: In vitro effects of canine Wharton’s jelly mesenchymal stromal cells and micellar nanoparticles on canine osteosarcoma D17 cell viability. Life Sciences, In Press, 2012.

Kansas State University

K-State

College of Veterinary Medicine