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College of Veterinary Medicine

Conference Program

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Sunday, June 4, 2017

Workshop: Translating NidoResearch into Field Applications

Session 1:  New Targets and Approaches for Antivirals

Moderators: Frank van Kuppeveld & Bob Rowland

08:15 – 08:25

Workshop opening remarks

08:25 – 08:50

 

 

W1. O-01 State of the art presentation

From Bench to Clinical Trial: microRNA 122 as an Antiviral Target for Hepatitis C Virus

Peter Sarnow*

Stanford University, CA, USA

08:50 – 09:10

 

 

W1. O-02 Discovery & Development of Coronavirus Protease Inhibitors as Potential Therapeutics

Andrew Mesecar*

Purdue University, IN, USA

09:10 – 09:30

 

W1. O-03  A Protease Inhibitor as a Potential Therapeutic for Feline Coronavirus

Yunjeong Kim*

 Kansas State University, KS, USA

09:30 – 09:45

 

 

 

W1. O-04 Broad-spectrum antiviral GS-5734 inhibits both epidemic and zoonotic coronaviruses

Timothy P. Sheahan1, Amy C. Sims1, Rachel L. Graham1, Vineet D. Menachery1, Lisa E. Gralinski1, James B. Case4, Sarah R. Leist1, Krzysztof Pyrc5, Joy Y. Feng2, Iva Trantcheva2, Roy Bannister2, Yeojin Park2, Darius Babusis2, Michael O. Clarke2, Richard L. Mackman2, Jamie E. Spahn2, Christopher A. Palmiotti2, Dustin Siegel2,Adrian S. Ray2, Tomas Cihlar2, Robert Jordan2, Mark R. Denison3, Ralph S. Baric1

1University of North Carolina at Chapel Hill, Chapel Hill, NC

2Gilead Sciences, Inc., Foster City, CA, 3-4Vanderbilt University Medical Center, Nashville, TN, 5Jagiellonian University, Krakow, Poland

09:45 – 10:00

 

 

W1. O-05 Utilizing yeast as a platform to identify therapeutics and genetic interactors for SARS and MERS-CoV

Stuart Weston, Krystal Matthews, Matthew Frieman

University of Maryland, MD, USA

 

10:00 – 10:30

Coffee Break

 

Session 2:  Opportunities for Nidovirus Vaccines

Moderators: Scott McVey & Pravina Kitikoon

10:30 – 10:50

 

W2. O-01 Overcoming Technical and IP Hurdles, from Concept to Product

Jay Calvert*

Zoetis Inc., USA

10:50 – 11:10

 

W2. O-02 Challenges in PRRS vaccine development and application in the developing world

Shishan Yuan*

Boehringer Ingelheim

11:10 – 11:30

 

W2. O-03 PRRSV vaccine development and application in China

Kegong Tian*

Pulike Inc. P. R. China

11:30 – 11:45

 

 

W2. O-04 Development of rationally attenuated live vaccines for effective control of infectious bronchitis

Erica Bickerton, Sarah Keep, Paul Britton

The Pirbright Institute, UK

11:45 – 12:00

 

 

 

W2. O-05 Protection against MERS-CoV infection by immunization with genetically engineered live-attenuated viruses

Javier Gutiérrez-Álvarez1, Carlos Castaño-Rodríguez1, Jose M. Honrubia1, Raul Fernandez-Delgado1, Sonia Zuñiga1, Paul McCray2, Stanley Perlman2, Isabel Sola1, Luis Enjuanes1

1National Center for Biotechnology (CNB-CSIC) Campus Universidad Autónoma de Madrid, Spain, 2University of Iowa, USA

 

12:00 – 13:30

Lunch roundtable session with industry and academic professionals

Chairs: Kyoung-Jin Yoon and Megan Niederwerder

 

Session 3: Host Genomics and Viral Infection

Moderators: Udeni Balasuria & Qiu Hong Wang

13:30 – 13:55

 

W3. O-01 State of the art presentation:  Genetic Engineering to answer basic questions about viral infection

Randall Prather*

University of Missouri, MO, USA

13:55 – 14:15

 

W3. O-02 Using animal breeding and genetic selection to combat infectious disease

Jack Dekkers*

Iowa State University, IA, USA

14:15 – 14:35

 

 

 

W3. O-03 Development of an Arterivirus RNA-based Platform Expression System

Kurt Kamrud*, Martina Felderman, Nancy Choi, Nathaniel Wang, Jessica Sparks, Lyn’Al Nosaka, Carolina Carpenter, Rebecca Mazahreh, Jason DeHart, Win Maung, Magda Barbu, Heather Gouvis, Bolyn Hubby

Synthetic Genomics Vaccines, Inc. CA, USA

14:35 – 14:50

 

 

W3. O-04 Parainfluenza Virus 5 (PIV5) As A Vaccine Vector: MERS-CoV Vaccine Development

Zhuo Li1, Kun Li2, Paul McCray2, Biao He1

1University of Georgia, GA; 2University of Iowa, IA, USA

 

14:50 – 15:20

Coffee Break

 

Session 4:  Evolution & Diagnostics of Nidoviruses

Moderators:  Hanchun Yang & DJ Rezac

15:20 – 15:40

 

 

W4. O-01 Translational outcomes of research into the biology of equine arteritis virus in advancing the diagnosis and control of viral arteritis

Udeni Balasuriya*

University of Kentucky, USA

15:40 – 16:00

 

W4. O-02 PRRSV evolution and challenges for diagnostics and epidemiology

Tomaz Stadejek*

Warsaw University of Life Sciences, Poland

16:00 – 16:15

 

 

 

W4. O-03 Rapid disease diagnostics and surveillance using a broad-spectrum microbial detection array

C.J. Jaing1, J.B. Thissen1, K.S. McLoughlin2, M.C. Niederwerder3, R. R.R. Rowland3 

1,2Lawrence Livermore National Laboratory, Livermore, CA, 3Kansas State University, Manhattan, KS, USA

16:15 – 16:30

W4. O-04 VSV pseudotype and monoclonal antibody-based assays for determining MERS coronavirus neutralizing antibody responses

Shuetsu Fukushi1, Aiko Fukuma1, Hideki Tani1, Takeshi Kurosu1, Satoshi Taniguchi1, Kazutaka Egawa1, Shumpei Watanabe1, Masayuki Shimojima1, Kazuya Shirato2, Shutoku Matsuyama2, Hanako Sekimukai3, Naoko Iwata-Yoshikawa3, Noriyo Nagata3, Kazuo Ohnishi4, Manabu Ato4, Hiroshi Sentsui5, and Masayuki Saijo1

1-4National Institute of Infectious Diseases and 5Department of Veterinary Medicine, Nihon University, Japan.

*Invited Speakers

 

18:00 – 18:15 Symposium opening remarks

 

18:15 – 19:00 Keynote presentation

New Insights into Zika Virus Pathogenesis, Immune Response and Vaccine Protection

Michael Diamond*

Washington University School of Medicine

St. Louis, Missouri, USA

 

19:00 – 22:00 Welcome Gala in the Marriott Hotel Barney Allis Historic Lobby

 

Monday, June 5, 2017

SESSION 1: Gymnastics of Viral Entry

Moderators: Tom Gallagher & Barney Graham

 

08:30 – 08:50

 

S1. O-01 Structural biology of betacoronavirus spike proteins

Andrew Ward*

The Scripps Research Institute, USA

 

08:50 – 09:10

S1. O-02 Structures and functions of coronavirus spike proteins: what lessons can we learn?

Berend-Jan Bosch*

Utrecht University, The Netherlands

 

 

 

 

09:10 – 09:25

S1. O-03 Structural studies of coronavirus spike proteins

Alexandra C. Walls1, M.Alejandra Tortorici2,3, Brandon Frenz1, Joost Snijder1, Wentao Li4, Peter Rottier4, Frank DiMaio1, Berend-Jan Bosch4, Félix A. Rey2,3, David Veesler1

1University of Washington, Seattle, Washington, USA; 2Institut Pasteur, Unité de Virologie Structurale, Paris, France; 3CNRS UMR 3569 Virologie, Paris, France; 4Utrecht University, The Netherlands

 

 

 

09:25 – 09:40

S1. O-04 Entry of human coronaviruses

Aleksandra Milewska1,2, Paulina Nowak1,2, Katarzyna Kosowicz1,2, Artur Szczepanski1,2, Krzysztof Pyrc1,2

1-2Jagiellonian University, Krakow, Poland

 

 

 

09:40 – 09:55

S1. O-05 Receptor engagement of the porcine epidemic diarrhea virus

Wentao Li, Frank J.M. van Kuppeveld,Peter J.M. Rottier, Berend-Jan Bosch

Utrecht University, The Netherlands

 

 

 

09:55 – 10:10    

S1. O-06 Structural insights into the SARS Spike-ACE2 receptor complex

Robert N. Kirchdoerfer1, Nianshuang Wang2, Jesper Pallesen1, Daniel Wrapp2, Hannah L. Turner1, Christopher A. Cottrell1, Kizzmekia S. Corbett3, Barney S. Graham3, Jason S. McLellan2, Andrew B. Ward1

1The Scripps Research Institute; 2Geisel School of Medicine at Dartmouth; 3National Institute of Allergy and Infectious Disease, USA

 

10:10 – 10:40  

Coffee Break

 

Moderators:  David Veesler & Berend-Jan Bosch

 

 

 

10:40 – 10:55

S1. O-07 The fusion peptide of the coronavirus spike protein has a novel bipartite organization and acts in a calcium-dependent manner

Alex L. Lai1, Jean K. Millet2, Jack H. Freed1, Susan Daniel3, Gary R. Whittaker2

1-3Cornell University, Ithaca NY 14853, USA

 

 

10:55 – 11:10

S1. O-08 Molecular cloning of porcine Siglec-3, Siglec-5 and Siglec-10 and identification of Siglec-10 as an alternative receptor for porcine reproductive and respiratory syndrome virus (PRRSV)

Jiexiong Xie, Isaura Christiaens, Bo Yang, Wander Van Breedam, Tingting Cui, Hans J. Nauwynck

Ghent University, Belgium

 

 

 

11:10 – 11:25

S1. O-09 A tetraspanin protein promotes coronavirus infection by linking cell receptors and proteases

James T Earnest1, Michael P Hantak1, Kun Li2, Paul B McCray Jr2, Stanley Perlman2,3, Tom Gallagher1

1Loyola University Medical Center, Maywood, IL; 2-3University of Iowa, Iowa City, IA, USA

 

 

 

11:25 – 11:40

S1. O-10 Wild type human coronaviruses prefer cell surface TMPRSS2 to endosomal cathepsins for cell entry

1Kazuya Shirato, 2Kazuhiko Kanou, 1Miyuki Kawase, 1Shutoku Matsuyama

1National Institute of Infectious Diseases, Tokyo, Japan; 2Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan

 

 

11:40 – 11:55

S1. O-11 Utilizing a glycan shield for epitope masking in HCoV-NL63 spike proteins

Alexandra C. Walls1, M.Alejandra Tortorici2,3, Brandon Frenz1, Joost Snijder1, Wentao Li4, Félix A. Rey2,3, Frank DiMaio1, Berend-Jan Bosch4, David Veesler1

1University of Washington, Seattle, Washington, USA; 2Institut Pasteur, Paris, France; 3CNRS UMR 3569 Virologie, Paris, France; 4Utrecht University, Utrecht, The Netherlands

 

12:00 – 13:30

Lunch Roundtable session: Young Scientists Career

Chair: Joan Lunney

 

SESSION 2: From Omics to Function

Moderators:  Brenda Hogue & Raoul de Groot

13:30 – 13:40

Women in Science Awards

 

13:40 – 14:00

S2. O-01 Applying Systems Biology to Viral Pathogenesis

Ralph Baric*

UNC Chapel Hill, North Carolina, USA

 

 

14:00 – 14:20

S2. O-02 Comparative Genomics and RNA Virus Gene Expression

Andrew E. Firth*1, Sawsan Napthine1, Roger Ling1, Nerea Irigoyen1, Betty YW. Chung2, John F. Atkins3, Ian Brierley1 

1-3University of Cambridge, UK

 

 

 

14:20 – 14:35 

S2. O-03 The viral predictome: partnering RNA structure and high-throughput analyses to predict novel coronavirus functional domains

Rachel L. Graham1, Wes Sanders2, Heather A. Vincent2, Jordan Texier2, Nat Moorman2, Dirk P. Dittmer2, Ralph S. Baric1,2

1-2The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

 

 

 

 

14:35 – 14:50 

S2. O-04 Identification of functional transcription regulatory sequences (TRS) in the SHFV genome by next generation sequencing

Han Di1, Joseph C. Madden1, Esther K. Morantz1, Rachel Graham2, Ralph S. Baric2,3, M. A. Brinton1

1Georgia State University, Atlanta, GA;2-3University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

 

14:50 – 15:20 

Coffee Break

 

Moderators:  Eric Snijder & Mark Denison

 

 

15:20 – 15:35 

S2. O-05 Coronavirus nsp14 modulates the innate immune response

Martina Becares, Alejandro Pascual-Iglesias, Isabel Sola, Luis Enjuanes, Sonia Zuñiga

National Center of Biotechnology (CNB-CSIC), Madrid, Spain

 

 

15:35 – 15:50 

S2. O-06 The 5’-terminal stem-loop 2 is a structurally and functionally conserved cis-acting RNA element in coronavirus genomes

Ramakanth Madhugiri, Nadja Karl, Daniel Petersen, John Ziebuhr

Justus Liebig University Giessen, Germany

 

15:50 – 16:05

S2. O-07 Activation of NF-kB by PRRSV is mediated by nucleocapsid protein binding to PIAS1

Hanzhong Ke, Dongwan Yoo

University of Illinois at Urbana-Champaign, Urbana, IL, USA

 

 

 

 

16:05 – 16:20

S2. O-08 Structure and kinetics of a core MERS papain-like protease with utility for structure-based drug design

Jozlyn Clasman1, Yahira M. Báez-Santos1,2, Robert C. Mettelman3, Amornrat O’Brien3, Susan C. Baker3, Andrew D. Mesecar1,4,5,6

1-2,4-5Purdue University, West Lafayette, IN; 3Loyola University Chicago, Stritch School of Medicine, Maywood, IL, USA

 

 

16:20 – 16:35

S2. O-09 Potent and selective inhibition of Middle East respiratory syndrome coronavirus papain-like protease by ubiquitin variants

Robert C. M. Knaap1, Wei Zhang2, Ben A. Bailey-Elkin3, Baldeep Khare3, Tim J. Dalebout1, Garrett G. Johnson3, Puck B. van Kasteren1, Nigel J. McLeish3, Jun Gu2, Wenguang He3, Brian L. Mark3, Sachdev S. Sidhu2, Marjolein Kikkert1

1Leiden University Medical Center, The Netherlands; 2University of Toronto, Toronto, Canada; 3University of Manitoba, Winnipeg, Canada

17:00 – 19:00

Poster Session #1.   Please put up posters on Mon AM, presenters should be at their posters from 17:00 – 19:00, posters stay up until Tuesday evening.

 

Tuesday, June 6, 2017

SESSION 3: How Viruses Take Over

Moderators: Volker Thiel & Marjolein Kikkert

 

 

08:30 – 08:50 

S3. O-01 Common themes in the biogenesis and structure of nidovirus replication organelles

Eric J. Snijder*

Leiden University Medical Center, The Netherlands

08:50 – 09:10 

S3. O-02 Tunneling nanotubes provide a unique conduit for intercellular spread of PRRSV infection: A novel pathways for nidovirus transmission

Ying Fang*

Kansas State University, USA

 

 

09:10 – 09:25 

S3. O-03 Proofreading-deficient coronaviruses evolve increased nucleotide selectivity over long-term passage

Kevin W. Graepel1, Xiaotao Lu2, James Brett Case1, Everett Clinton Smith3,Mark R. Denison1,2

1-2Vanderbilt University Medical Center, Nashville, TN; 3The University of the South, Sewanee, TN, USA

 

09:25 – 09:40 

S3. O-04 Identification of sites of Infectious Bronchitis Virus RNA synthesis

Nicole Doyle, Selma Rayon, Helena J Maier

The Pirbright Institute, UK

 

 

09:40 – 09:55 

S3. O-05 Role of MERS-CoV nonstructural protein 1 in virus replication

Keisuke Nakagawa1, Krishna Narayanan1, Kumari G. Lokugamage1, Masami Wada1, Ralph S. Baric2, Shinji Makino1

1University of Texas Medical Branch, Galveston, TX; 2 University of North Carolina at Chapel Hill, NC, USA

 

09:55 – 10:25 

Coffee Break

 

 

 

10:25 – 10:45 

S3. O-06 Inhibition of Replication and Pathogenesis of Coronaviruses by Targeting Viral Methyltransferases

Shilei Wang1, Cong Zeng1, Yi Wang1, Ruangang Pan1, Yu Chen1, Deyin Guo*2

1Wuhan University, Wuhan, China; 2Sun Yat-sen University, Guangdong, China.

 

 

 

10:45 – 11:00 

S3. O-07 The Epigenetic Landscape during Coronavirus Infection

Alexandra Schäfer1, Vineet D. Menachery1, Katrina M. Waters2, Amy C. Sims1, Timothy P. Sheahan1, Ralph S. Baric 1 

1University of North Carolina at Chapel Hill, Chapel Hill, NC; 2Pacific Northwest National Laboratory, Richland, WA, USA

 

 

 

11:00 – 11:15 

S3. O-08 Composition of the subcellular molecular environment of coronavirus replicase complexes identified by proximity labelling.  

Philip V'kovski1,2, Sophie Braga Lagache3, Cedric Simillion4,5, Alexey Stukalov6, Andreas Pichlmair6, Manfred Heller3, Volker Thiel1,7

1-5,7University of Bern, Switzerland; 6Max-Planck Institute of Biochemistry, Martinsried, Germany

 

11:15 – 11:30 

S3. O-09 The MHV packaging signal promotes virulence and prevents a robust interferon response during infection

Jeremiah Athmer, Anthony Fehr, Mathew Grunewald, Stanley Perlman

University of Iowa, Iowa City, Iowa, USA

 

11:30 – 11:45 

S3. O-10 Special presentation: National Bio and Agro-defense Facility (NBAF)

Ron Trewyn, Kansas State University, Manhattan, KS, USA

 

11:45 – 13:00

Lunch

 

SESSION 4: Emerging & Evolving Viruses

Moderators:  John Ziebuhr & Alexander Gorbalenya

13:00 – 13:20 

S4. O-01 Origins and emergence of coronaviruses

Christian Drosten*

University of Bonn Medical Centre, Bonn, Germany

13:20 – 13:40 

S4. O-02 The nsp14-exoribonuclease in coronavirus  replication, pathogenesis and evolution

Mark Denison*

Vanderbilt University School of Medicine, Nashville, TN, USA

 

 

 

 

13:40 – 13:55 

S4. O-03 Cooperation and competition among genome regions in macroevolution of nidoviruses

Ivan A. Kuznetsov1, Anastasia A. Gulyaeva2, Svetlana Iarovenko1, Chris Lauber2,3, Andrey M. Leontovich4, Igor A. Sidorov2, Alexander E. Gorbalenya1,2,4

1,4Lomonosov Moscow State University, Moscow, Russia; 2Leiden University Med. Center, Leiden, The Netherlands; 3Technische Universität Dresden, Dresden, Germany

 

 

13:55 – 14:10 

S4. O-04 Betacoronavirus adaptation to humans involved progressive loss of hemagglutinin-esterase lectin activity

Yifei Lang1, Mark J.G. Bakkers1, Louris J. Feitsma2, Ruben J.G. Hulswit1, Stefanie A.H. de Poot1, Arno L.W. van Vliet1, Irina Margine1, Jolanda D.F. de Groot-Mijnes3, Frank J.M. van Kuppeveld1, Martijn A. Langereis1, Eric G. Huizinga2, Raoul J. de Groot1

1-3Utrecht University, The Netherlands

 

14:10 – 14:25 

S4. O-05 Novel nido-like virus genomes associated with eukaryotic intracellular RNA pools

Benjamin W. Neuman1, Khulud Bukhari2, Saad T. Mutlk2, Hasan S. H. Alrashedi2, Ban O. Abdulsattar2, Guocheng Shu3, Lanying Zhao3, Jianping Jiang3, Leonid L. Moroz4, Federica di Palma5, Nadia Ayoub6, Jessica Garb7, Weilin Sun8, Barry Pittendrigh8

1Texas A&M University–Texarkana, TX, USA; 2University of Reading, UK; 3Chinese Academy of Sciences, Chengdu, China; 4University of Florida, USA; 5The Earlham Institute, Norwich, UK; 6Washington & Lee University, Lexington, VA, USA; 7University of Massachusetts-Lowell, MA, USA; 8Michigan State University, East Lansing, MI, USA

 

 

14:25 – 14:40 

S4. O-06 Examining the emergence potential of MERS-like CoV PDF-2180

Menachery VD1, Yount, B.L2, Scobey, T2, Dinnon, K.H2,3,  Anthony, S.J4,5,6, Gilardi, K7, Goldstein, T7, Ssebide, B8, Mbabazi, R8, Navarrete-Macias, I4, Liang, E4,6, Wells, H4, Hicks, A4, Petrosov, A4, Byarugaba, D.K9,10, Debbink, K2, Randell, S.H11, Cranfield7, M, Johnson, C.K7, Lipkin, W.I4,5,  Mazet, J.A.K7, Baric, R.S2,3

1University of Texas Medical Branch, Galveston, Texas; 2-3,11University of North Carolina; 4Mailman School of Public Health, Columbia University, New York, NY; 5Mailman School of Public Health,Columbia University,New York, NY; 6EcoHealth Alliance, NY, New York; 7One Health Institute & Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California Davis, CA; 8Gorilla Doctors, c/o MGVP, Inc., Davis, CA; 9Makerere University Walter Reed Project, Kampala, Uganda; 10Makerere University, College of Veterinary Medicine, Kampala, Uganda

15:00 –  

Scenic views of Kansas City

Attendees sign up at the registration desk for a group tour of Powell Gardens or enjoy exploring KC on your own during the afternoon and evening.

 

Wednesday, June 7, 2017

SESSION 5: How the Host Responds to Viruses

Moderators: Ralph Baric & Luis Enjuanes

08:30 – 08:50 

S5. O-01 Viral antagonists of innate immune responses

Susan Baker*

Loyola University Chicago, Stritch School of Medicine, USA

 

 

08:50 – 09:10 

S5. O-02 OAS-RNase L: an antiviral and proapoptotic pathway

Susan R. Weiss*

University of Pennsylvania, USA

 

09:10 – 09:25 

S5. O-03 TiPARP upregulation during CoV infection reveals potential activation of the aryl hydrocarbon receptor

Matthew Grunewald, Anthony Fehr, Stanley Perlman

University of Iowa Carver College of Medicine, USA

 

 

 

09:25 – 09:40 

S5. O-04 The interleukin-1 pathway contributes to SARS-CoV pathogenesis and disease

Jacob Kocher1, Anne Beall1, Kara Jensen1,Jessica Plante1, Lisa Gralinski1, Vineet Menachery1, Alexandra Schäfer1, Timothy Sheahan1, Trevor Scobey1, Ralph Baric1,2

1-2University of North Carolina-Chapel Hill, Chapel Hill, NC, USA

 

 

 

09:40 – 09:55 

S5. O-05 A naturally occurring recombinant enterovirus expresses torovirus protease

Pengcheng Shang1, Saurav Misra2, Ben Hause1, Ying Fang1

1-2Kansas State University, Manhattan, KS, USA 

 

 

 

 

09:55 – 10:10 

S5. O-06 Middle East respiratory coronavirus accessory protein 4a inhibits PKR-mediated antiviral stress responses

Huib H. Rabouw1, Martijn A. Langereis1, Robert C.M. Knaap2, Tim J. Dalebout2, Peter J. Bredenbeek2, Marjolein Kikkert2, Raoul J. de Groot1, and Frank J.M. van Kuppeveld1

1-2Utrecht University, The Netherlands

 

10:10 – 10:40 

Coffee Break

 

Moderators:  Susan Weiss & Dongwan Yoo

 

10:40 – 10:55 

S5. O-07 PARP-dependent ADP-ribosylation independently enhances the IFN response and represses coronavirus replication

Anthony R. Fehr1, Gytis Jankevicius2, Craig Fett1, Ivan Ahel2, Stanley Perlman1

1University of Iowa, Iowa City, IA; 2University of Oxford, UK

 

10:55 – 11:10 

S5. O-08 Infectious bronchitis virus (IBV) accessory protein 4b and stress granules

Ross Hall1, Julian A. Hiscox2, Paul Britton1, Helena J. Maier1

1The Pirbright Institute, UK; 2Institute of Infection and Global Health, University of Liverpool, Liverpool, UK

 

 

11:10 – 11:25

S5. O-09 Type I interferons and alveolar macrophages protect hDPP4 KI mice from mouse-adapted strain of MERS-CoV infection

Rudragouda Channappanavar1, Anthony R Fehr1, Kun Li2, Paul B McCray2,1 and Stanley Perlman1,2

1-2University of Iowa, Iowa City, USA

 

 

 

11:25 – 11:40

S5. O-10 DPP4-mediated immunity during MERS-CoV infection: implication for development of medicalcountermeasure

Algaissi AA1,6, Agrawal AS1, Han S2, Chan TS1, Peng B-H3, Couch RB4, Tseng CTK1,5

1-5University of Texas Medical Branch at Galveston, TX; 6Jazan University, Saudi Arabia

 

 

11:40 – 11:55

S5. O-11 The unfolded protein response induced by porcine reproductive and respiratory syndrome virus infection of alveolar macrophages is involved in immune dysregulation

W.Y. Chen, W.M. Schniztlein, G. Calzada-Nova, F.A. Zuckermann

University of Illinois at Urbana-Champaign. Urbana, IL, USA

 

12:00 – 13:30

Lunch

 

SESSION 6: Insights into Viral Pathogenesis

Moderators:  Wen-hai Feng & Isobel Sola

13:30 – 13:50 

S6. O-01 Historical context and biological enigma of rhinovirus C

Ann Palmenberg*

University of Wisconsin-Madison, USA

13:50 – 14:10 

S6. O-02 Illuminating the coronavirus replicase: from pathogenicity to molecular function

Volker Thiel*,

University of Bern, Switzerland

14:10 – 14:25 

S6. O-03 Characterization of virus - host interaction dynamics within the respiratory epithelium

Ronald Dijkman1,2, Hulda R. Jonsdottir1,2, Volker Thiel1,2

1-2University of Bern, Switzerland

 

14:25 – 14:40 

S6. O-04 Coronavirus deubiquitinase modulates type I IFN response in infected macrophages and promotes infection in vivo

Xufang Deng1, Anna M Mielech1, Yafang Chen2, Robert C Mettelman1, Matthew Hackbart1, Amornrat O’Brien1, Andrew D Mesecar2, Susan C Baker1

1Loyola University Chicago Stritch School of Medicine, Maywood, IL; 2Purdue University, West Lafayette, IN, USA

 

14:40 – 14:55 

S6. O-05 CXCL16 gene variants regulate equine arteritis virus infection in stallions

Sanjay Sarkar1, Ernest Bailey1, Yun Young Go1,2, R. Frank Cook1, Ted Kalbfleisch3, John Eberth1, R. Lakshman Chelvarajan1, Kathleen M. Shuck1, Sergey Artiushin1, Peter J. Timoney1, Udeni B. R. Balasuriya1

1University of Kentucky, Lexington, KY 40546, USA; 2Korea Research Institute of Chemical Technology, Daejeon, South Korea; and 3University of Louisville, Louisville, KY, USA

 

14:55 – 15:25 

Coffee Break

 

 

15:25 – 15:45 

S6. O-06 Pathogenesis of Emerging Coronavirus

Vincent Munster*

NIH/NIAID/Rocky Mountain Laboratories, USA

 

15:45 – 16:00 

S6. O-07 Mutations in a nidoviral trans-membrane replicase subunit confer resistance towards the cyclophilin inhibitor cyclosporin A

Adriaan de Wilde1, Jessika Zevenhoven-Dobbe1, Corrine Beugeling1, Linda Boomaars-van der Zanden1, Montserrat Bárcena2, Eric Snijder1, Clara Posthuma1

Leiden University Medical Center, The Netherlands

 

16:00 – 16:15 

S6. O-08 Critical amino acids in nsp9 and nsp10 determining the fatal virulence of Chinese highly PRRSV

Lei Xu, Lei Zhou, Weifeng Sun, Pingping Zhang, Xinna Ge, Xin Guo, Jun Han, Hanchun Yang

College of Veterinary Medicine, China Agricultural University

 

16:15 – 16:30 

S6. O-09 Coronavirus Gene Expression and Cellular Differential Expression Analyses by RNA Sequencing and Ribosome Profiling

Nerea Irigoyen1, Andrew E. Firth1, Adam M. Dinan1, Krzysztof Franaszek1, Joshua D. Jones1, Betty YW. Chung2, Stuart G. Siddell3, Ian Brierley1

1-2University of Cambridge, UK; 3University of Bristol, UK

 

16:30 – 16:45 

S6. O-10 Structural and biochemical insights into the interaction of coronavirus papain-like proteases and interferon-stimulated-gene-product 15 from different species

Courtney M. Daczkowski1, John Dzimianski1, Nick J. Mank1, Phani Das2, Kay Faaberg2, Scott D. Pegan1

1University of Georgia, Athens,
Georgia, 2National Animal Disease Center, ARS, USDA, Ames, IA, USA

17:00 – 19:00 

Poster Session #2.   Please put up posters on Wed AM, presenters should be at their posters from 17:00 – 19:00, posters stay up until Thursday afternoon.

 

20:00 – 22:00      ICTV Arterivirus and Coronavirus Study Group Meetings

 

Thursday, June 8, 2017

SESSION 6: Insights into Viral Pathogenesis

Moderators:  Kay Faaberg & Bart Haagmans

 

08:30 – 08:50 

S6. O-11 Human Coronavirus Virulence Motifs and Virulence

Luis Enjuanes*

Centro Nacional de Biotecnologia, CNB-CSIC, Madrid, Spain

 

 

08:50 – 09:05 

S6. O-12 Interplay between coronavirus and nonsense-mediated mRNA decay pathway

Masami Wada, Kumari G. Lokugamage, Krishna Narayanan, Shinji Makino

The University of Texas Medical Branch, Galveston, Texas, USA

 

 

 

 

09:05 – 09:20 

S6. O-13 Inhibition of cytosolic phospholipase A2alpha impairs coronavirus replication by interfering with virus-induced replicative organelle formation

Christin Müller1, Martin Hardt2, Dominik Schwudke3, Benjamin W. Neuman4, Stephan Pleschka1, John Ziebuhr1*

1-2Justus Liebig University Giessen, Germany; 3Research Center Borstel, Leibniz Center for Medicine and Bioscience, Borstel, Germany; 4Texas A&M University, Texarkana, TX, USA

 

 

 

09:20 – 09:35

S6. O-14 Double-stranded viral RNA as a potential mediator for the persistence of porcine reproductive and respiratory syndrome virus

Rui Guo, Xinyu Yan, Pengcheng Shang, Tao Wang, Ying Fang

Kansas State University, Manhattan, KS, USA

 

 

 

 

 

09:35 – 09:50

S6. O-15 Confined DPP4 expression in the respiratory tract of macaques is associated with restricted MERS-CoV replication

W. Widagdo1, Lidewij C.M. Wiersma1, Debby Schipper1, V. Stalin Raj1 , Albert D.M.E. Osterhaus2, Judith M.A. van den Brand1, Bart L. Haagmans1

1Erasmus Medical Center, Rotterdam, The Netherlands; 2University of Veterinary Medicine, Hannover, Germany.

*The authors contributed equally to this work.

 

 

 

 

09:50 – 10:05

S6. O-16 SARS-CoV-encoded small RNAs contribute to infection-associated lung pathology

Lucía Morales1, Juan Carlos Oliveros2, Raúl Fernandez-Delgado1, Benjamin Robert tenOever3, Luis Enjuanes1, Isabel Sola1

1-2National Center of Biotechnology (CNB-CSIC), Madrid, Spain; 3Icahn School of Medicine at Mount Sinai, New York, USA

 

10:05 – 10:35

Coffee Break

 

SESSION 7: Host Genomes and Animal Model Systems

Moderators:  Linda Saif & Gary Whittaker

 

10:35 – 10:55

S7. O-01 Animal Coronaviruses: Interspecies transmission, virulence and cell tropism determinants

Linda Saif*

The Ohio State University, USA

 

10:55 – 11:15

S7. O-02 Pigs, PRRSV, host genomics and CRISPR

Raymond Rowland*

Kansas State University, USA

 

 

 

 

 

 

 

11:15 – 11:30

S7. O-03 A mouse adapted MERS-Coronavirus causes lethal lung disease in human DPP4 knock-in mice

Kun Li1, Christine L. Wohlford-Lenane1, Rudragouda Channappanavar2, Jung-Eun Park3, James T. Earnest3, Thomas B. Bair4, Amber M. Bates5, Kim A. Brogden5, Heather A. Flaherty7, Tom Gallagher3, David K. Meyerholz6, Stanley Perlman1,2, Paul B. McCray, Jr.1,2

1-2,4-7University of Iowa, Iowa City, IA; 3Loyola University Chicago, Maywood, IL, USA

 

 

 

11:30 – 11:45

S7. O-04 Acute respiratory infection in human dipeptidyl peptidase 4 transgenic mice infected with Middle East respiratory syndrome coronavirus

Naoko Iwata-Yoshikawa1, Tadashi Okamura1,2, Yukiko Shimizu2, Hanako Sekimukai1,3, Shuetsu Fukushi1, Tadaki Suzuki1, Yuko Sato1, Makoto Takeda1, Masato Tashiro1, Hideki Hasegawa1, Noriyo Nagata1

1National Institute of Infectious Diseases, Tokyo, Japan; 2National Center for Global Health and Medicine Tokyo, Japan; 3Tokyo University of Agriculture and Technology, Tokyo, Japan

 

 

 

 

11:45 – 12:00

S7. O-05 Development of a New Human DPP4 Transgenic Mice Model for Studying Lung Pathogenesis of MERS-CoV Infection

Anurodh S. Agrawal1, Abdullah Algaissi1, Maki Wakamiya2, Bi-Hung Peng3, Teh-Sheng Chan1, Robert B. Couch4, Chien-Te K. Tseng1,5

1-5University of Texas Medical Branch, Galveston, Texas, USA

12:00 – 13:30

Lunch session-TWiV:  This Week in Virology with Vincent Racaniello

 

SESSION 8: Counter measures to Disease

Moderators:  Margo Brinton & Shinji Makino

 

13:30 – 13:50

S8. O-01 Role of T cell responses in Coronavirus Clearance and Protection

Stanley Perlman*

University of Iowa, USA

 

13:50 – 14:10

S8. O-02 Challenges of controlling porcine reproductive and respiratory syndrome by vaccination

Hans Nauwynck*

Gent University, Belgium

 

 

 

 

 

 

 

14:10 – 14:25

S8. O-03 Evaluation of stabilized prefusion MERS-CoV spike trimers as vaccine candidates

Kizzmekia S. Corbett1, Joan Ngwuta1, Benjamin I. Cabrera1, Nianshuang Wang2, Jesper Pallesen3,Daniel Wrapp2, Lingshu Wang1, Wei Shi1, Yi Zhang1, Syed Moin1, Robert N. Kirchdoerfer3, Hannah L. Turner3, Christopher A. Cottrell3, Timothy Sheahan4, Adam Cockrell4, Andrew B. Ward3, Jason S. McLellan2,Wing-Pui Kong1, Ralph Baric4, Barney S. Graham1

1National Institutes of Health; 2Geisel School of Medicine at Dartmouth; 3The Scripps Research Institute; 4University of North Carolina-Chapel Hill, USA

 

 

 

 

14:25 – 14:40

S8. O-04 Protective efficacy of a simian adenovirus vaccine against MERS-CoV challenge in a transgenic human-DPP4 mouse model

Michael Letko1, Vincent J. Munster1, Daniel Wells2, Teresa Lambe2, Robert J. Fischer1, Trenton Bushmaker1, Greg Saturday3, Neeltje van Doremalen1, Sarah C. Gilbert2, Emmie de Wit1, George M. Warimwe2,4,5

1,3NIH Rocky Mountain Laboratories, Hamilton, Montana, USA; 2The Jenner Institute, University of Oxford, UK; 4The Pirbright Institute, Woking, UK; 5KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya

 

 

 

 

 

 

14:40 – 14:55

S8. O-05 Identification of MERS-CoV neutralizing nanobodies by

direct cloning from a bone marrow cDNA library

V. Stalin Raj1, Nisreen Okba1, Javier Gutierrez-Alvarez2, Dubravka Drabek3, Widagdo Widagdo1, Mart M. Lamers1, Isabel Sola2, Albert Moise Bensaid4, Joaquim Segalés i Coma4, Berend Jan Bosh5, Marion Koopmans1, Gerd Sutter6, Albert D.M.E. Osterhaus7, Luis Enjuanes2, Bart L. Haagmans1

1,3Erasmus Medical Center, Rotterdam, the Netherlands; 2Department of Molecular and Cell Biology, CNB-CSIC, Madrid, Spain; 4Centre de Recerca en Sanitat Animal (CReSA), Barcelona, Spain; 5Utrecht University, The Netherlands; 6Institute for Infectious Diseases and Zoonoses, University of Munich, Germany; 7Center for Infection Medicine and Zoonoses Research, University of Veterinary Medicine, Hannover, Germany

 

14:55 – 15:25

Coffee Break

 

Moderators:  Stanley Perlman & Hans Nauwynck

 

 

 

 

 

 

15:25 – 15:40

S8. O-06 Neutralizing mAbs targeting distinct domains of MERS-CoV Spike and their roles in viral neutralization

Lingshu Wang1, Wei Shi1, M. Gordon Joyce1, Yi Zhang1, Masaru Kanekiyo1, James D. Chappell2, Michelle M. Becker2, Misook Choe1, Rosemarie D. Mason1, Tongqing Zhou1, Kevin O. Saunders1, Kathleen M. Tatti3, Lia M. Haynes3, Mark R. Denison2, Kayvon Modjarad4, Wing-Pui Kong1, John R. Mascola1, Barney S. Graham1

1National Institutes of Health, Bethesda, MD; 2Vanderbilt University Medical Center, Nashville, TN; 3Centers for Disease Control and Prevention, Atlanta, GA; 4Walter Reed Army Institute of Research, Silver Spring, MD

 

 

 

 

15:40 – 15:55

S8. O-07 Protection from MERS-CoV-induced pulmonary disease with neutralizing antibody depends on virus dose and intervention timing

Adam S. Cockrell1, Madeline Douglas1, Trevor Scobey1, Davide Corti3, Ralph S. Baric1,2

1,2University of North Carolina-Chapel Hill, North Carolina, USA; 3Humabs BioMed SA, Bellinzona, Switzerland

 

 

15:55 – 16:10

S8. O-08 Nonstructural Protein 2 and Papain-like Protease 2 Chimeras between Highly-Pathogenic JXwn06 and Ingelvac PRRS® MLV Strains of Porcine Reproductive and Respiratory Syndrome Virus

Fengxue Wang1, Nicholas Otis1, Jude Chenge2, Stephanie Bester2, Scott D. Pegan2, Kay S. Faaberg1

1National Animal Disease Center, ARS, USDA, Ames, IA, USA and 2University of Georgia, Athens, Georgia, USA

 

 

 

 

16:10 – 16:25

S8. O-09 Targeting proteases and interferon antagonists for coronavirus therapeutics

Robert C. Mettelman1, Xufang Deng1, Sarah E. St. John2, Gary R. Whittaker3, Andrew D. Mesecar2, Susan C. Baker1

1Loyola University Chicago, Stritch School of Medicine, Maywood, IL; 2Purdue University, West Lafayette, IN; 3Cornell University, Ithaca, NY, USA

 

16:25 – 16:40

S8. O-10 Synthetic vector-mediated IgG generated in vivo confers protective immunity against viral infection

Kar Muthumani

The Wistar Institute, Philadelphia, PA, USA

16:40 – 16:55

ICTV meeting report

Alexander Gorbalenya

Leiden University Med. Center, Leiden, The Netherlands

18:00 – 23:00

Gala Dinner and Concert  in The Grand Hall at Power & Light

 

Friday, June 9, 2017

Departure

 

Printable Program (PDF format)