KSUCVM • Continuing Education
 


The 21st Meeting of the American Society for Rickettsiology

Saturday - Tuesday, September 8 - 11, 2007


Meeting Schedule:

Saturday | Sunday | Monday | Tuesday


Saturday, September 8, 2007

3:00 - 6:00 pm Registration, Colorado Springs Marriott, Foyer
6:00 pm Welcome Reception (Provided with Conference)
7:30 pm Welcome: Michael Minnick, President, American Society for Rickettsiology
 
 Keynote Speaker Introduction: James Samuel, Vice President, American Society for Rickettsiology
 
 Keynote Address: Craig Roy, Associate Professor of Microbial Pathogenesis at Yale University School of Medicine
 
Identification and Characterization of Type IV Effector Proteins Encoded by Intracellular Pathogens
 

Sunday, September 9, 2007

7:30 - 8:15 am Poster Session I Set-up
Genetic Manipulation
Session Moderators: Abdu Azad and Urlike Munderloh
8:15 am Mariner-Based Transposon Mutagenesis of Rickettsia prowazekii
ZhiMei Liu, Aimee M. Tucker, Lonnie O. Driskell, and David O. Wood
8:30 am Selection of Anaplasma phagocytophilum Transformants with an Herbicide Resistance System 
Roderick F. Felsheim, Michael J. Herron*, Ulrike G. Munderloh
8:45 am Targeted Genetic Transformation of Wolbachia pipientis Using Homologous Recombination
Inaki Iturbe-Ormaetxe*, Jasmin Howie and Scott L. O’Neill
9:00 am Establishment of Molecular Tools Useful in Creating Mutations by Homologous Recombination in Ehrlichia chaffeensis
Kamesh R. Sirigireddy*, Chuanmin Cheng, Lalitha Peddireddi and Roman R. Ganta
9:15 am The Target Genes for Knockout to Attenuate Rickettsia prowazekii
Jian-zhi Zhang, David H. Walker, David O. Wood, and Xue-jie Yu*
9:30 am An Unexpected Anaplasma marginale Transformant
Roderick F. Felsheim, Guy Palmer, Anthony F. Barbet, Adela S. O. Chavez, Timothy J. Kurtti, Ulrike G. Munderloh*
9:45 - 10:30 am Refreshment Break and Poster Viewing in Poster Session I
Clinical Aspects, Diagnosis and Treatment
Session Moderators: Stephen Graves and Ranjan Premaratna
10:30 am Bartonella Species as a Potential Cause of Neurological Dysfunction in Immunocompetent People
EB Breitschwerdt*, RG Maggi, WL Nicholson, NA Cherry
10:45 am Reasons for Delay in the Diagnosis of Rickettsial Infections in Sri Lanka; Results of a Preliminary Study
R. Premaratna*, M.S. Bailey, A. D. Loftis, G.A. Dasch, H. J. de Silva
11:00 am Two Novel Rickettsiae Identified in Australia
Leonard H. Izzard*, Stephen R. Graves, and John Stenos
11:15 am Rocky Mountain Spotted Fever in Argentina
Christopher D. Paddock*, Susana Fernandez, Gustavo Echenique, John W. Sumner, Will K. Reeves, and Carlos E. Remondegui.
11:30 am Q Fever in Australia and the USA Connection
Stephen Graves*, Aminul Islam, John Ferguson, Michelle Lockhart, Chelsea Nguyen and John Stenos
11:45 am Diagnostic Aspects of Scrub Typhus in an Endemic Setting: Why Does It Remain Such a Problem?
Stuart D. Blacksell*, Daniel H. Paris, Kemajittra Jenjaroen, Nuntipa Aukkanit, Nicholas P.J. Day
12:00 n Lunch (Provided with Conference)
1:00-2:00 pm Poster Session I (with presenters)

Existence of Similar Plasmids in Diverse Spotted Fever Group Rickettsiae Suggests Potential for a Rickettsial Plasmid Transformation Vector
Gerald D. Baldridge*, Nicole Y. Burkhardt, Roderick F. Felsheim, Timothy J. Kurtti, and Ulrike G. Munderloh

Optimization of HIMAR Based Anaplasma phagocytophilum Transformation
Michael J. Herron*, Roderick F. Felsheim, Ulrike G. Munderloh

Green Fluorescent Protein Reporter Gene Expression under Regulation of the ompB Promoter in Transgenic Rickettsia montanensis
Nicole Y. Burkhardt*, Gerald D. Baldridge, Adela S. Oliva, Timothy J. Kurtti, and Ulrike G. Munderloh

Plasmid Construction and Evaluation of Transposon-based Mutagenesis in Ehrlichia chaffeensis
Kendra S. Siebert*, Kamesh R. Sirigireddy, Chuanmin Cheng and Roman R. Ganta

Preliminary Evaluation of a Peptide-based Assay for Detection of Ehrlichia ewingii Antibodies in Experimentally and Naturally-infected Dogs.
D. Daniluk, J. Slauenwhite, T. O’Connor, M.J. Yabsley, D.S. Adams, P.P.V.P. Diniz, E.B. Breitschwerdt, S. Gaunt, R. Chandrashekar*

Comparison of Sensitivity and Specificity of Normal Real-time PCR and One-step Nested Real-time Assays for Detection of Bartonella Species
James M. Colborn*, Kristen Van Wyk, and Michael Y. Kosoy

Q Fever from Unknown Febrile Patients in Korea
Young Sill Choi*, Kyu Jam Hwang, Sang Hee Park, Soo Kyoung Shim and Mi Yeoun Park

Evaluation of Electrochemiluminescence (ECL) as a Diagnostic Method for Acute Rickettsioses
Gary Wen and Juan P. Olano

Endemic and Travel Cases of Fatal Israeli Spotted Fever: The Importance of PCR Diagnosis
M. E. Eremeeva*, M. Weinberger, A. Karas, C. D. Paddock, A. Keysary, C. Strenger, M. Leitner, J. Ty Chai, C. Borland

Reliable Methods to Quantify Typhus Group Rickettsiae
Colette Keng, Leonora Orejuela, Erika Gomez, Paula Correa, and Gustavo Valbuena*

Blood Culture-negative Bartonella quintana endocarditis Requiring Heart Valve Replacement: First Reported Case of Bartonella endocarditis in Sweden.
Christian Ehrenborg*, Svante Hagberg, Jakob Alden, Signar Mäkitalo, Gunnar Myrdal, Erik Larsson, Eva Hjelm and Goran Friman

Diagnosis of Bovine Anaplasmosis in Beef Herds
James B. Reinbold*, Kamesh Sirigireddy, Johann F. Coetzee, and Roman Ganta

Antimicrobial Effect of a Cathelicidin Host defense peptide, Fowlicidin-1 (5-26), on Coxiella burnetii
Saugata Mahapatra*, Glenn Zhang, and Edward I. Shaw

Experimental Inoculation of Dogs with a Human or Canine Isolate of Anaplasma phagocytophilum and Molecular Evidence of Persistent Infection Following Doxycycline Therapy
H. Wamsley*, A. Barbet, J. Abbott, A. Lundgren, R. Marlow, R. Chandrashekar, M. Beall, K. Cyr, A. Alleman

Got Q? Not Just for Farms Anymore Amanda Candee

Isolation and Identification of a Bartonella melophagi-like Species from a Woman Ricardo G. Maggi*, Michael Kosoy, Edward B Breitschwerdt

A Sybr Green-Based Real-time PCR Protocol for Brucella sp., Coxiella bunettii and Francisella tularensis Detection in Canine Blood Samples P.P.V.P. Diniz*, H. Marr, A.J. Birkenheuer

Epidemic Typhus in Colombia Leonora Orejuela,* Marylin Hidalgo, Jorge Hernandez, and Gustavo Valbuena

Identification and Characterization of Tetratricopeptide Repeat Proteins found in the genome of Orientia tsutsugamushi Sun-Young Bang*. Bon-A Cho, Chan-Ki Min, Jung-Hee Lee, Se-Yoon Kim, Myung-Sik Choi, Ik-Sang Kim, Nam-Hyuk Cho

The Internalization of O. tsutsugamushi is Mediated by the Cooperative Interactions of its Major Outer Membrane Proteins with Multiple Host Ligands Jung-Hee Lee*, Se-Yoon Kim, Sun Young Bang, Bon-A Cho, Chan-Ki Min, Nam-Hyuk Cho, Myung-Sik Choi, Ik-Sang Kim

Rickettsia rickettsii Infection Protects Human Microvascular Endothelial Cells Against Staurosporine-induced Apoptosis by a cIAP2-independent Mechanism Jeremy R. Bechelli *, Elena Rydkina, and Sanjeev K. Sahni

Transcriptional Regulation of the Heme Binding Protein Gene Family of Bartonella quintana is Accomplished by a Novel Promoter Element and Iron response regulator (Irr) James M. Battisti*, Laura S. Smitherman, Kate N. Sappington, Nermi L. Parrow, Rahul Raghavan, and Michael F. Minnick

Expression of Anaplasma marginale MSP2 Antigenic Variants during Culture in Tick and Mammalian Cells A.S. Oliva-Chavez,* U.G. Munderloh, T.J. Kurtti, R. Felsheim, N. Burkhardt

Assessment of Flea Bite and Flea Feces Transmission of a Rattus-associated Bartonella Strain to Laboratory Mice Leah Colton*, Kenneth Gage, and Michael Kosoy

Identification of Histone 2B as a Putative Rickettsia felis Receptor on ISE6, Ixodes scapularis-derived Tick Cells Chutima Thepparit,* Apichai Bourchookarn, Walairat Pornwiroon, Kevin R. Macaluso

Anaplasma phagocytophilum-Borrelia burgdorferi Coinfection: Synergistic Protease, Chemokine and Cytokine Expression at the Human Blood-brain Barrier Dennis J. Grab,* Diana G. Scorpio, Nicole C. Barat, J. Stephen Dumler

Drosophila S2 Cells as a Model for Studying Ehrlichia chaffeensis Infections Alison L. Fedrow*, Tonia Von Ohlen, Daniel Boyle, Roman R. Ganta, Stephen K. Chapes

Early Host Cell Interactions and Phagosome Escape by Rickettsia typhi Rebecca Maag* and Abdu Azad

PCR-based Identification of Non-culturable Bartonella Species in Rodent Blood Kristen Van Wyk*, Michael Kosoy, Christina Morway, James Colborn, Pamela Reynolds

Tragedy at CDC: Reflections on Accidental Exposure to Rickettsia rickettsii Thirty Years Later William L. Nicholson*, Christopher D. Paddock, Richard Dixon, and David Sencer

Co-phylogenetic Analysis of Anaplasma phagocytophilum and Its Vectors, Members of the Ixodes ricinus Complex Nathan C. Nieto,* Janet Foley, Patrick Foley, and Mike B. Teglas

Dynamics of Growth and Infectivity of Rickettsia parkeri, R. montanensis, and Rickettsia amblyommii (Wb-8-2) in Mammalian and Tick Cell Lines Andre S. Zanetti,* Piyanate Sunyakumthorn, Kevin R. Macaluso

Expression of Surface Cell Antigens (Sca) During Rickettsia typhi in vitro Infection Khandra Sears*, Shane. M. Ceraul, M. Sayeedur Rahman and Abdu F. Azad

Clearance and Persistence of Ehrlichia canis in Dogs Treated with Rifampin or Doxycycline John J. Schaefer, G. R. Needham, J. Kahn, W. G. Bremer, Y. Rikihisa, S. A. Ewing, J.C. McClure, M.L. Crothers and R. W. Stich*

Effect of Bartonella bacilliformis Infection on Cytokine Production in THP-1 Macrophages Laura Hendrix, Hermien Berhout, Ian Martinez and Maaike Moen

In vitro Culture of the Ixodes ovatus Ehrlichia Ulrike G. Munderloh*, David J. Silverman, Gilbert G. Ahlstrand, Rachel Racine, Katherine C. McNamara, Madhumouli Chatterjee, and Gary M. Winslow

Comparative Analysis of Vertebrate and Invertebrate α-tubulin in Response to Infection with Rickettsia parkeri Natthida Petchampai,* Chutima Thepparit and Kevin R. Macaluso

Two High Molecular Weight Anaplasma phagocytophilum Proteins Localize to the Bacterial Inclusion Membrane and are Differentially Expressed throughout the Course of Infection Matthew J. Troese*, Dexter V. Reneer, Jason A. Carlyon

Anaplasma phagocytophilum MSP2 (P44)-18 Predominates and is Modified into Multiple Isoforms in Human Myeloid Cells Matthew J. Troese, Madhubanti Sarkar, Matthew J. Troese, Sarah A. Kearns, Dexter V. Reneer, and Jason A. Carlyon*

Differential Expression of Anaplasma phagocytophilum MSP2 (P44) Paralogs is Induced upon Cultivation in Sialyl Lewis x-deficient HL-60 Cells Matthew J. Troese, Madhubanti Sarkar, Sarah A. Kearns, Dexter V. Reneer, and Jason A. Carlyon*

Characterization of Rickettsia rickettsii Type IV Secretion ATPase, VirB11 Betsy Kleba* and Ted Hackstadt

Coxiella burnetii Interaction with the Polymorphonuclear Leukocyte Joshua Hill*, Kasi E. Russell-Lodrigue, James E. Samuel

Comparative Analysis of Endothelial Host Cell Responses to Spotted Fever Group Rickettsiae: Implications for Pathogenesis Michael E. Woods and Juan P. Olano*

Identification and Characterization of Ankyrin Repeat Proteins found in the genome of Orientia tsutsugamushi Bon-A Cho*, Sun-Young Bang, Chan-Ki Min, Jung-Hee Lee, Se-Yoon Kim, Myung-Sik Choi, Ik-Sang Kim, Nam-Hyuk Cho

Regulation of Inducible Heme Oxygenase and Cyclooxygenase Isozymes in a Mouse Model of Spotted Fever Group Rickettsiosis Elena Rydkina *, Loel C. Turpin, David J. Silverman, and Sanjeev K. Sahni

Characteristics of Unique Rickettsia rickettsii Iowa Clones Tina R. Clark*, Damon W. Ellison, Ted Hackstadt

Function and Regulation of the Hemin Uptake Locus of Bartonella Quintana Nermi L. Parrow*, James M. Battisti, Laura S. Smitherman, Kate N. Sappington and Michael F. Minnick

PCR Detection of Bartonella bovis in the Blood of North Carolina Beef Cattle NA Cherry*, RG Maggi, AL Kennedy, EB Breitschwerdt

Prospects of Louse-borne Typhus Eradication in Ukraine I.Kurhanova

A Study of Q Fever - In Ukraine Z.G.Kushnir*, N.G.Bek, T.S.Lutsyk Presented by Dr. I. Kurhanova
 

Host Vector Pathogen Interactions

Session Moderators: Amanda Read and Juan Martinez

2.00 pm Transcription of Anaplasma phagocytophilum P44 Paralogs in Sheep during Primary and Secondary Bacteraemia RJ Thomas*, CA Forrester, RJ Birtles, AD Radford, Z Woldehiwet
2:15 pm Characterization of the OmpB and Ku70 Interaction during Rickettsia conorii Infection of Host Cells Yvonne Chan, Douglas Gilbert and Juan J. Martinez*
2:30 pm Anaplasma phagocytophilum Strains in the Western US May Comprise a Genospecies Group Janet Foley*, Nate Nieto, and John Madigan
2:45 pm Infection Load Dynamics of Rickettsia felis in Actively Feeding Ctenocephalides felis Kathryn E. Reif *, Rhett W. Stout, Gretchen C. Henry and Kevin R. Macaluso
3:00 pm A Tick Midgut Specific Kunitz-Type Serine Protease Inhibitor and Its Role in Rickettsia montanensis Infection and Growth Shane M. Ceraul*, Sheila M. Dreher-Lesnick, M. Sayeedeur Rahman and Abdu F. Azad
3:15pm Detection of Anaplasma Phagocytophilum in Ixodid Ticksfrom Equine Habitats in the Southeastern United States of America Dawn M. Roellig* and Quentin Fang
3:30 pm The Role of Complement Receptor 3 (CR3) in the Pathogenesis of Coxiella burnetii Nine Mile Phase II Lung Infection A. J. Read*, J. J. Sentissi, S. M. Erickson and A. G. Harmsen
3:45 pm Free Time
6:00 - 7:00 pm ASR Business Meeting, Elections
7:00 pm Dinner and Evening Reception (Provided with Conference)
9:00 pm Remove Posters

Monday, September 10, 2006

7:45 - 8:15 pm Poster Session II Set-up

Genetics, Physiology and Metabolism

Session Moderators: Kevin Macaluso and Rahul Raghavan

8:15 am Pseudogene Repertoire and Superinfection Kelly A. Brayton*, James E. Futse, and Guy H. Palmer
8:30 am Two rDNA Introns Contribute to the Slow Growth of Coxiella burnetii Rahul Raghavan*, Sherry A. Coleman, Linda D. Hicks, James M. Battisti and Michael F. Minnick
8:45 am A Biochemical Study of the Energetic Requirements of the Rickettsia prowazekii GTP/GDP Transporter Belonging to the Translocase Family of Nucleotide Carriers Rosemary A. W. Roberts, Herbert H. Winkler and Jonathon P. Audia*
9:00 am Amplification of New Australian Isolates of Coxiella burnetii in Four Different Cell Lines Michelle Lockhart,* Aminul Islam, Stephen Graves and John Stenos
9:15 am Prediction of Sec-dependent Secreted Proteins in Rickettsia conorii (Malish 7) Using Bioinformatics Tools Joshua Shallom*, Nataraj Dongre, Oswald Crasta, Joseph J. Gillespie, Abdu F. Azad,Joao Setubal and Bruno Sobral
9:30 am Transmission of Rickettsia amblyommii by the Lone Star tick, Amblyomma americanum, during Bloodmeal Acquisition Laura Sarradet, Andre S. Zanetti, Timothy Morgan, Phillip C. Williamson, Kevin R. Macaluso*
9:45-10:30 am Refreshment Break and Poster Viewing in Poster Session II

Genomics and Proteomics

Session Moderators: Gregory Dasch and Gwi-Moon Seo

10:30 am Identification of Methylated Proteins in Typhus Group Rickettsiae
Chien-Chung Chao and Wei-Mei Ching*
10:45 am Programmed OMP Sequence Variation in Lambs Experimentally Infected with Anaplasma phagocytophilum Erik G. Granquist*, Snorre Stuen, Anna M. Lundgren, Margrethe Bråhen, Anthony F. Barbet
11:00 am Genetic Comparison of Riesia pediculicola from Head and Body Lice
G.A. Dasch*, J. M. DeJarnette, S. E. Karpathy, D. L. Reed, F. White, M. Zambrano, and E. Kirkness
11:15 am Limited Genetic Variation Found among Isolates of Rickettsia amblyommii in Intergenic Region Sequences Sandor E. Karpathy*, Tonya Mixson-Hayden, Scott R. Campbell, Jerome Goddard, Michael L. Levin, Christina Mikosz, Terry L. Schulze, Mary Spratt, and Gregory A. Dasch
11:30 am Remodeling of the Surface Proteome of Anaplasma marginale within Tick Cells
Susan M. Noh*, Kelly A. Brayton, Donald P. Knowles, Joseph T. Agnes, Guy H. Palmer
11:45 am Sequencing and Characterization of pRAK1, a 24.4 Kb Plasmid from Rickettsia akari
Marina E. Eremeeva*, Anup Madan, Tyeshia Halsell, and Gregory A. Dasch
12:00 n Lunch (Provided with Conference)
1:00-2:00 pm Poster Session II (with presenters)
  Identification and Characterization of Rickettsia typhi Secretory Proteins
Nicole C. Ammerman*, M. Sayeedur Rahman, Abdu F. Azad

The Coxiella burnetii rpoH Gene is Induced to High Levels during Early Infection in vitro
Sherry A. Coleman*, Linda D. Hicks, and Michael F. Minnick

Discovery and Characterization of a Novel Group I Intron in the 23S rDNA of Coxiella burnetii
Rahul Raghavan*, Scott R. Miller, Linda D. Hicks and Michael F. Minnick

Characterization and Growth of Pleomorphic Rickettsia felis in a Tick Cell Line
Piyanate Sunyakumthorn*, Apichai Bourchookarn, Walairat Pornwiroon, Kevin R. Macaluso

Rickettsial Infections and their Clinical Presentations in the Western Province of Sri Lanka: A Hospital Based Study
R. Premaratna*, A.D. Loftis, T.G.A.N. Chandrasena, G.A. Dasch, H.J. de Silva

Coxiella burnetii Nine Mile Phase II Infection of Wild Type and SCID Mice as Models of Pulmonary Q Fever A. J. Read*, S. M. Erickson, L. A. McNamee, J. J. Sentissi and A. G. Harmsen

HGA Associated with Nosocomial Transmission in Anhui Province, PR China 2006
Zhang Lijuan*, Liu Yan, Chen Qun, Luan Mingchun, Fu Xiuping ,Zhang Jingshan, Wan Kanglin, Jiang Xiugao, Jing HuaiQi, Hai Rong, Yu Dongzheng, Ni Daxin, Feng Zijuan,Yang Weizhong, Wang Yu, and Xu Jianguo and J. Stephen Dumler

Development of a Quantitative Real-time PCR Assay to Detect Candidatus Rickettsia andeanae
Ju Jiang and Allen L. Richards*

Decontamination Issues with Coxiella burnetii: An Evaluation of Currently Available Disinfectants
Rachael A. Priestley*, Robert F. Massung

The Complementation of the plsC Gene from Coxiella burnetii
Nathan B. Unsworth* and James E. Samuel

Evaluating the Transcriptome of Rickettsia typhi
Jennilee B. Robinson*, Vladimir L. Motin, Donald. H. Bouyer, Marina E. Eremeeva, and Gregory A. Dasch

Real Time RT-PCR Analysis of Coxiella burnetii Type IV Secretion System Genes icmW, icmV, icmT during Infection of Host Cells
John Kent Morgan* and Edward I. Shaw

Gene Expression Profiles of Orientia tsutsugamushi and the Comparative Analysis of Metabolic Properties within the Order of Rickettsiales
Chan-Ki Min*, Jae-Seong Yang, Bon-A Cho, Jung-Hee Lee, Se-Yoon Kim, Sun young Bang, Sanguk Kim, Siv G. E. Andersson, Myung-Sik Choi, Ik-Sang Kim, Nam-Hyuk Cho

Genome-scale Metabolic Analysis of Rickettsia prowazekii
Ranjan Srivastava and Wei-Mei Ching*

Temporal and spatial patterns of Bartonella infection in black-tailed prairie dogs (Cynomys ludovicianus)
Ying Bai*, M.Y. Kosoy, C. Ray, R. J. Brinkerhoff, and S. K. Collinge

First Molecular Documentation of Bartonella sp. Infection in Dogs from Greece
P.P.V.P. Diniz*, T. Petanides, M.E. Mylonakis, A.F. Koutinas, E.B. Breitschwerdt

Characterization of the Anaplasma phagocytophilum Heat Shock Operon Groesl from Infected Dogs, Cats, and Horses in the Eastern USA (2005 to 2007) PPVP Diniz*, R Chandrashekar, EB Breitschwerdt

Prevalence to Tick-borne Organisms in Dogs from Northeastern Arizona (2005 and 2007) PPVP Diniz*, M Beall, K Omark, R Chandrashekar, D Daniluk, K Cyr, JF Koterski, RG Robbins, P Lalo (Komalestewa), B Hegarty, EB Breitschwerdt

Novel Method for the Quantitative Detection of Ehrlichia sp. in Ohio Amblyomma americanum ticks Robert Fitak, Daryl J. Kelly*, Mary K. Daniels, Amanda D. Loftis, Paul A. Fuerst

Rickettsial Prevalence and Antigenic Variation in Ohio-collected Amblyomma americanum Ticks
Daryl J. Kelly* Robert Fitak, Mary K. Daniels, Monica J. Crary, Paul A. Fuerst

Sequencing and Analysis of Orientia tsutsugamushi DNA in a Leptotrombidium ¬pallidum Mite Colony Originating in a Scrub Typhus Endemic Region in Saitama Prefecture, Japan Daryl J. Kelly,* Robert Fitak, Mamoru Takahashi, Hitoko Misumi, Paul A. Fuerst

Serological Reactivity of 47 kDa Outer Membrane Protein of Orientia tsutsugamushi with Scrub Typhus Patient Sera Hua-Wei Chen*, Elissa Mutumanje, Hong Ge and W.-M. Ching

Evidence for NK Cells, but Not T cells, as Determinants in the Outcome of Ehrlichiosis Emily C. Crossley*, Heather L. Stevenson, Nahed Ismail, and David H. Walker

Th1-dominant Response with Transient Immunosuppression in Acute Coxiella burnetii Infection in the Guinea Pig Aerosol-challenge Model
Kasi E. Russell-Lodrigue*, Hyosun Cho, Christine T. McFarland, Guoquan Zhang, Masako Andoh, Heather L. Briggs, David N. McMurray, and James E. Samuel

Anaplasma marginale Proteins: Recognition by Infection Antisera, Crossreactions with Anti-Anaplasma phagocytophilum Infection Antisera, and Host Cell-Associated Differential Immunoreactivity Heather L. Wamsley*, Annie L. Moreland, A. Rick Alleman, Katherine M. Kocan, Ulrike G. Munderloh, Anthony F. Barbet

Ehrlichia chaffeensis Proteins Associated with Ehrlichia-containing Vacuolar Membrane
Yong Zhu* and Xuejie Yu

Immunoreactive Protein Diversity in Globally Distributed Ehrlichia canis Strains Xiaofeng Zhang*, Avi Keysary, Tian Luo, Gad Baneth, Carmela Strenger, Trevor Waner, Marcelo Labruna, and Jere W. McBride

Protective Heterologous Immunity against Fatal Ehrlichiosis and Enhanced Immunopathogenesis Following Homologous Challenge Nagaraja Thirumalapura*, David H. Walker, and Nahed Ismail

Mechanisms of Antibody-mediated Immunity to Coxiella burnetii Jeffrey G. Shannon*, Diane Cockrell, Dale Howe and Robert A. Heinzen

The Presence of Either CD4+ or CD8+ T Cells is Sufficient to Control Pulmonary Infections with Coxiella burnetii Nine Mile Phase II S. M. Erickson*, A. J. Read, J. J. Sentissi, A. G. Harmsen

Immunization with E. muris P28 Outer Membrane Proteins Recombinant DNA Provides Protection Against Homologous Challenge and is Dependent on IFN-γ Producing CD4+ Effector Memory T Cells P.A. Crocquet-Valdes*, N. Thirumalapura, H.L. Stevenson, X-J Yu, C. Keng, N. Ismail and D.H. Walker

Genome Sequences of Two Human Q Fever Endocarditis Isolates (K and G) and a Naturally Attenuated Dugway Isolate of Coxiella burnetii Paul. A. Beare*, Stephen F. Porcella, John Kupko, Nathan Unsworth, James E. Samuel, and Robert A. Heinzen

Genomic Comparison of Virulent Rickettsia rickettsii Sheila Smith and Avirulent Rickettsia rickettsii Iowa
Damon W. Ellison*, Tina R. Clark, Daniel E. Sturdevant, Kimmo Virtaneva, Stephen F. Porcella and Ted Hackstadt

Global Gene Transcription Analysis of Anaplasma phagocytophilum in Tick and Human Cells Using Tiling Microarrays Curtis M. Nelson*, Michael J. Herron, Roderick F. Felsheim, Ulrike G. Munderloh, Timothy J. Kurtti

Total, Membrane, and Immunogenic Proteins of Macrophage- and Tick Cell-derived Ehrlichia chaffeensis Evaluated by LC-MS/MS and MALDI-TOF Methods
Gwi-Moon Seo*, Chuanmin Cheng, John Tomich and Roman R. Ganta

Identification of Ehrlichia chaffeensis gp200 Target Genes by Chromotin Immunopreciptation and Microarray Hybridization (ChIP on chip) Bing Zhu*, Xiaofeng Zhang, Jere W. McBride

Mass Spectrometry Analysis of Native Ehrlichia chaffeensis gp47 Purified from Cell Culture Supernatants by 2D-Electrophoresis Abdul Wakeel*, Xiaofeng Zhang, and Jere W. McBride

Proteome Analysis of Rickettsia parkeri Strain Portsmouth by Two-dimensional Gel Electrophoresis and Mass Spectrometry Walairat Pornwiroon*, Apichai Bourchookarn, Kevin R. Macaluso

Comparative Phylogenomics of Rickettsia
Joseph J. Gillespie*, Kelly Williams, Eric E. Snyder, Eric Nordberg, Shane M. Ceraul, Chitti Dharmanolla, Daphne Rainey, Jeetendra Soneja, Joshua M. Shallom, Maulik Shukla, Nataraj Dongre Vishnubhat, Rebecca Wattam, Anjan Purkayastha, Michael Czar, Oswald Crasta, Joao Setubal, Abdu F. Azad, Bruno S. Sobral

Detection of Potentially Novel Bartonella species in Amblyomma americanum (Acari: Ixodidae) Sarah A. Billeter*, Melissa Miller, Edward B. Breitschwerdt, and Michael G. Levy

Predicting the Emergence of Tick-borne Infections Based on Climate Changes in Korea
Joon-seok Chae*, Jennifer Zipser Adjemian, Heung-Chul Kim, Sung-jin Ko, Terry A. Klein, Janet Foley

Isolation of Bartonella vinsonii sups. berkhoffii Type III from a Military Working Dog Maria B Cadenas*, Ricardo G. Maggi, Barbara C. Hegarty and Edward B Breitschwerdt

Investigation of the Flea-borne Rickettsioses in California Erica K. Hayes*, Ashley M. Williams, Sandor E. Karpathy, Renjie Hu, Laura Krueger, Stephen Bennett, Nelson Kerr, Wesley Moore, Gregory A. Dasch, Marina E. Eremeeva

Prevalence and Dynamics of Four Tick-borne Agents in Amblyomma americanum from Atlanta Suburbs (Georgia) Michael L. Levin*, Danielle R. Troughton

Recent Increases in Reported Cases of Rocky Mountain Spotted Fever in the United States John W Krebs* John J Openshaw, Robert C Holman, Eric Mandel, and David L Swerdlow

Host Species Tropism of Anaplasma phagocytophilum Correlates with Different Sequence Types of the ank Gene Wiebke Scharf, Sonja Schauer, Yvonne Kern, Christian Bogdan, Friederike von Loewenich*

Got Q? Detection of Coxiella burnetii in Commercially Sold Raw Milk, USA Amanda D. Loftis*, Rachael A. Priestley, Amanda J. Candee, Amy M. Denison, Nicole E. Patterson, and Robert F. Massung

National Investigation of Orientia tsutsugamushi from Vector in Korea
SK Shim, EN Choi, KO Yu, HJ Park, CM Kim, KH Lee, JK Park, PH Park, MH Yoon, NS Song, CK In, SC Im, GM Choi, SH Park, YS Choi, KJ Hwang, HK Im and MY Park*

Surveillance of Rickettsiae in Humans and Wild Rodents in Singapore Yap G.*, Johansson P., Siew C.C., Low H.T. and Ng L.C
 

Ecology, Epidemiology and Infection in Animals
Session Moderators: Daryl Kelly and Amanda Loftis
2:00 pm Detection and Identification of Spotted Fever Group Rickettsiae in Dermacentor Ticks from Southern California Mary E. Wikswo*, Renjie Hu, Gregory A. Dasch, Laura Krueger, Aaron Arugay, Keith Jones, Barry Hess, Stephen Bennett, Marina E. Eremeeva
2:15 pm Isolation of Rickettsia amblyommii and Seroprevalence of Rickettsia in the State of Veracruz, Mexico Aaron Medina-Sanchez*, Edilma Guevara, Virginia Alcantara, Carlos Garza, Farron Hunt, Jeanine Davis, Rosario Gonzalez, Donald Bouyer, David Walker
2:30 pm Comparative Strain Analysis of Anaplasma phagocytophilum Infection and Clinical Outcomes in a Canine Model of Human Granulocytic Anaplasmosis Diana G. Scorpio*, J. Stephen Dumler, Nicole C. Barat, Judith A. Anastasio
2:45 pm Dynamic and Stochastic Transmission of Numerous Anaplasma phagocytophilum Genotypes among Lambs in an Endemic Infected Sheep Flock Georgia A F Ladbury, Snorre Stuen*, Rachael Thomas, Kevin J Bown, Zerai Woldehiwet, Erik G Granquist, and Richard J Birtles
3:00 pm Natural and Experimental Infection of White-tailed Deer (Odocoileus virginianus) with an Ehrlichia sp. from the United States Closely Related to Ehrlichia ruminantium Michael J. Yabsley*, Amanda D. Loftis, and Susan E. Little
3:15 pm Retrospective Survey for Q Fever in French Guiana and Propositions for an Epidemiologic Surveillance System Claire Grangier, Marion Debin, Françoise Ravachol, Vanessa Ardillon, Christine Simonnet, Frédéric Queuche, Jacques Rosine, Sonia Laouira, Pierre-Edouard Fournier, Isabelle Quatresous, Marine Herbert, Eric Legrand, Geneviève Guillot, Anne-Marie Bourbigot, Félix Djossou, Philippe Quénel, Didier Raoult, Jacques Morvan, Jean-Baptiste Meynard*
3:30 pm Ten Years of Ticks Submitted to the Human Tick Test Kit Program E.Y. Stromdahl*, S.R. Evans and M.A. Vince
3:45 pm Geographic Distribution and Genetic Diversity of the Newly Discovered Panola Mountain Ehrlichia sp. Amanda D. Loftis*, Tonya R. Mixson, Ellen Y. Stromdahl, Michael J. Yabsley, Laurel E. Garrison, Phillip C. Williamson, Robert Fitak, Paul Fuerst, and Keith Blount
4:00 pm Seasonal Dynamics of Bartonella Species in Woodrats and Their Fleas Christina Morway*, Michael Kosoy, Kelly Sheff, John Montenieri, Pam Reynolds, and Nelson Powers

Evening Free

9:00 pm Remove Posters

Tuesday, September 11, 2007

Immunity and Vaccine Development

Session Moderators: Wendy Brown and Joao Pedra

8:15 am

Species-Specific Antibody Epitopes in Terminal Acidic Domains of Ehrlichia canis gp200
Kimberly A. Nethery, C. Kuyler Doyle, Xiaofeng Zhang, and Jere W. McBride*

8:30 am

ASC/PYCARD and Caspase-1 Regulate the IL-18/IFN- Axis During Anaplasma phagocytophilum Infection
Joao H. F. Pedra*, Fayyaz S. Sutterwala, Bindu Sukumaran, Yasunori Ogura, Feng Qian, Ruth R. Montgomery, Richard A. Flavell, and Erol Fikrig

8:45 am

Differential Interaction of Dendritic Cells with Rickettsia: Impact on Host Susceptibility to Murine Spotted Fever Rickettsiosis
Rong Fang*, Nahed Ismail, Lynn Soong, Vsevolod L. Popov, Ted Whitworth, Donald H. Bouyer, David H. Walker

9:00 am

Establishing the Infection Model of Scrub Typhus in Rhesus Monkey (Macaca mulatta)
Kriangkrai Lerdthusnee*, Montip Gettayacamin, Rawiwan Imerbsin, Kwanta Chayapumh, Taweesak Monkanna, Surachai Leepitakrat, Warisa Leepitakrat, Sucheera Insuan, Pranee Hansukjariya, Weerayut Charoensongsermkit, Nittaya Khlaimanee and Jittawadee R. Murphy

9:15 am 

The Control of Anaplasma phagocytophilum in vivo is Dependent on CD4+ T Cells, but is Independent of Typical Th1 Cytokines and a Wide Spectrum of Effector Mechanisms
Katharina Becher, Yvonne Kern, Birte Steiner, Christian Bogdan and Friederike von Loewenich*

9:30 am

Rapid Identification of Novel Anaplasma marginale T-cell Immunogens Expressed using Cell-free in vitro Transcription and Translation
Wendy C. Brown*, Job E. Lopez, Paul A. Beare, Junzo Norimine, and Robert A. Heinzen

9:45-10:30 am Refreshment Break
Pathogenesis
Session Moderators: Jacob Ijdo and Jose Garcia-Garcia
10:30 am Regulation of Expression of the Bartonella quintana Vomp Trimeric Autotransporter Adhesin Family
Peng Zhang, Greg M. Dolganov, Helen Gerns, Erin Heinemeyer, Jeanna S. Goo, and Jane E. Koehler*
10:45 am In situ Detection of Anaplasma by DNA Target-Primed Rolling Circle Amplification of Padlock Probes
H. Wamsley*, A. Alleman, J. Abbott, A. Barbet
11:00 am Epigenetic Control of Host Cell Gene Expression by the Rickettsial Pathogen Anaplasma phagocytophilum Facilitates its Intracellular Survival and Propagation
Jose C. Garcia-Garcia*, Shaaretha Pelly, Sarah Tremley, Nicole Barat, J. Stephen Dumler
11:15 am SH2 Domain Interactions with AnkA during Anaplasma phagocytophilum Infection
Jacob W. IJdo* and Trent Waugh
11:30 am Syk-independent Infection by Anaplasma phagocytophilum
Dexter V. Reneer, Sarah A. Kearns, Madhubanti Sarkar, and Jason A. Carlyon*
11:45 am Route of Infection and Phylogenetic Group-specific Virulence of Isolates Determines Disease Outcome in a Q Fever Mouse Model 
Masako Andoh*, Victoria Baxter, Chen Chen, Kasi E. Russell-Lodrigue, Guoquan Zhang, James E. Samuel

12:00 n

Meeting Adjourned

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